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İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi ve Rejeneratif Tıpta Uygulanabilirliği

Yıl 2021, Cilt: 47 Sayı: 1, 117 - 126, 01.04.2021
https://doi.org/10.32708/uutfd.801247

Öz

2006 yılında Takahashi ve Yamanaka dört transkripsiyon faktörünün (Oct4, Sox2, Klf4 ve c-Myc) fibroblast hücrelerine aktarılması ve bu transkripsiyon faktörlerinin ifadesinin pluripotent kök hücre elde etmek için yeterli olduğunu bildirmiş ve somatik hücrelerin geriye programlanarak elde edilen bu hücreler indüklenmiş pluripotent kök hücreler (İPKH) olarak adlandırılmıştır. Daha sonraki yıllarda transkripsiyon faktörleri ve yeniden programlama şartlarının optimizasyonu ile ilgili birçok çalışma yapılmıştır. Bugüne kadar farklı somatik hücrelere transkripsiyon faktörlerinin farklı metotları ile tanıtımı ya da transkripsiyon faktörlerinin farklı kombinasyonlarının kullanımının etkisi araştırma konusu olmuştur. Somatik hücrelerin yeniden programlanması amacı ile birçok farklı vektör sistemi bulunmaktadır. Bu vektör çeşitlerinin İPKH eldesi için verimlilikleri birbirlerinden farklılık göstermektedir. Bu derlemede, kök hücrelerin genel özellikleri ve uygulama alanlarının irdelenmesinin yanı sıra ağırlıklı olarak indüklenmiş pluripotent kök hücrelerinin elde edilmesi üzerinde durulmuştur. Ayrıca İPKH’lerin klinik amaçlı kullanım potansiyellerine de değinilmektedir.

Destekleyen Kurum

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Proje Numarası

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Teşekkür

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Kaynakça

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Generation of Induced Pluripotent Stem Cells and Applications in Regenerative Medicine

Yıl 2021, Cilt: 47 Sayı: 1, 117 - 126, 01.04.2021
https://doi.org/10.32708/uutfd.801247

Öz

In 2006, Takahashi and Yamanaka reported that the transfer and expression of four transcription factors (Oct4, Sox2, Klf4, and c-Myc) into fibroblast cells were sufficient to obtain cells similar to embryonic stem cells. The cells obtained by reprogramming of somatic cells are called induced pluripotent stem cells (IPSC). In the following years, various studies were carried out for the optimization of transcription factors and reprogramming conditions. Until now, the introduction of transcription factors to different somatic cells or stem cells by various methods or the effect of using different combinations of transcription factors has been the subject of research. There are many different vector systems to reprogramme somatic cells. The efficiencies of the vector types for obtaining IPSC differ from each other. In this review, the general properties of stem cells and their application areas are discussed, as well as the acquisition of induced pluripotent stem cells and their potential for clinical use.

Proje Numarası

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Kaynakça

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  • 3. Deshmukh RS, Kovács KA, Dinnyés A. Drug discovery models and toxicity testing using embryonic and induced pluripotent stem-cell-derived cardiac and neuronal cells. Stem Cells Int. 2012. doi:10.1155/2012/379569
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  • 22. Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature. 1981. doi:10.1038/292154a0
  • 23. Shen H, Zhang L, Liu M, Zhang Z. Biomedical applications of graphene. Theranostics. 2012;2(3):283-294. doi:10.7150/thno.3642
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  • 32. Pralong D, Mrozik K, Occhiodoro F, et al. A novel method for somatic cell nuclear transfer to mouse embryonic stem cells. Cloning Stem Cells. 2005;7(4):265-271.
  • 33. Fulka J, Loi P, Fulka H, Ptak G, Nagai T. Nucleus transfer in mammals: Noninvasive approaches for the preparation of cytoplasts. Trends Biotechnol. 2004. doi:10.1016/j.tibtech.2004.04.002
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  • 36. Seyalioğlu İ, Şenel Eraslan B, Hot İ, Demircan YT, Çetin G. Klonlamaya Genetik, Etik ve Hukuksal Açıdan Yaklaşım. Adli Tıp Derg. 2007.
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  • 39. Takahashi K, Tanabe K, Ohnuki M, et al. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. Cell. 2007. doi:10.1016/j.cell.2007.11.019
  • 40. Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science (80- ). 2007. doi:10.1126/science.1151526
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Toplam 82 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Biyokimya ve Hücre Biyolojisi (Diğer)
Bölüm Derleme Makaleler
Yazarlar

Nevra Cesur 0000-0003-3979-6053

Nelisa Laçin Türkoğlu 0000-0003-3176-0902

Proje Numarası -
Yayımlanma Tarihi 1 Nisan 2021
Kabul Tarihi 18 Ocak 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 47 Sayı: 1

Kaynak Göster

APA Cesur, N., & Laçin Türkoğlu, N. (2021). İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi ve Rejeneratif Tıpta Uygulanabilirliği. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 47(1), 117-126. https://doi.org/10.32708/uutfd.801247
AMA Cesur N, Laçin Türkoğlu N. İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi ve Rejeneratif Tıpta Uygulanabilirliği. Uludağ Tıp Derg. Nisan 2021;47(1):117-126. doi:10.32708/uutfd.801247
Chicago Cesur, Nevra, ve Nelisa Laçin Türkoğlu. “İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi Ve Rejeneratif Tıpta Uygulanabilirliği”. Uludağ Üniversitesi Tıp Fakültesi Dergisi 47, sy. 1 (Nisan 2021): 117-26. https://doi.org/10.32708/uutfd.801247.
EndNote Cesur N, Laçin Türkoğlu N (01 Nisan 2021) İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi ve Rejeneratif Tıpta Uygulanabilirliği. Uludağ Üniversitesi Tıp Fakültesi Dergisi 47 1 117–126.
IEEE N. Cesur ve N. Laçin Türkoğlu, “İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi ve Rejeneratif Tıpta Uygulanabilirliği”, Uludağ Tıp Derg, c. 47, sy. 1, ss. 117–126, 2021, doi: 10.32708/uutfd.801247.
ISNAD Cesur, Nevra - Laçin Türkoğlu, Nelisa. “İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi Ve Rejeneratif Tıpta Uygulanabilirliği”. Uludağ Üniversitesi Tıp Fakültesi Dergisi 47/1 (Nisan 2021), 117-126. https://doi.org/10.32708/uutfd.801247.
JAMA Cesur N, Laçin Türkoğlu N. İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi ve Rejeneratif Tıpta Uygulanabilirliği. Uludağ Tıp Derg. 2021;47:117–126.
MLA Cesur, Nevra ve Nelisa Laçin Türkoğlu. “İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi Ve Rejeneratif Tıpta Uygulanabilirliği”. Uludağ Üniversitesi Tıp Fakültesi Dergisi, c. 47, sy. 1, 2021, ss. 117-26, doi:10.32708/uutfd.801247.
Vancouver Cesur N, Laçin Türkoğlu N. İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi ve Rejeneratif Tıpta Uygulanabilirliği. Uludağ Tıp Derg. 2021;47(1):117-26.

ISSN: 1300-414X, e-ISSN: 2645-9027

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