Investigation of Dose-Dependent Effect of Curcumin on T-47D Breast Cancer Cells

Yıl 2021, Cilt: 47 Sayı: 2, 241 - 245, 01.08.2021

Nuriye Ezgi Bektur Aykanat Sedat Kaçar

https://doi.org/10.32708/uutfd.963295

Öz

Curcumin, a polyphenol derived from turmeric belonging to the Zingiberaceae family, has multiple effects, including anti-inflammatory, antitumor, anti-oxidative and antimicrobial effects. There are many studies on the effects of curcumin on different cancer cells. This study aims at investigating the anti-cancer effect of curcumin on T-47D breast cancer cell. T-47D breast cancer cells will be examined by MTT assay and inverted microscopy. IC50 doses are 65.8 μM for 24 h-long MTT, 46.4μM for 48 h-long MTT and 26.6 μM for 72 h-long MTT assay. In the inverted microscope, curcumin-treated cells appear to be rounded and detached from the bottom flask with a massive morphology. Our results showed that curcumin significantly reduced T-47D cell proliferation. Curcumin may be a candidate for breast cancer therapy, either alone or in combination with other molecules. In the future, more comprehensive and multicenter-supported advanced clinical studies are needed to elucidate the mechanism of action of curcumin on breast cancer cells.

Anahtar Kelimeler

Curcumin, Breast Cancer, T-47D Breast Cancer Cells

T-47D Meme Kanseri Hücreleri Üzerinde Kurkuminin Doz Bağımlı Etkisinin İncelenmesi

Öz

Zingiberaceae familyasına ait zerdeçaldan elde edilen bir polifenol olan kurkumin, anti-inflamatuar, anti-tümör, anti-oksidatif ve antimikrobiyal etkiler dahil olmak üzere birçok etkiye sahiptir. Kurkuminin farklı kanser hücreleri üzerindeki etkileri hakkında birçok çalışma bulunmaktadır. Bu çalışma, kurkuminin T-47D meme kanseri hücre canlılığı üzerindeki anti-kanser etkisini araştırmayı amaçlamaktadır. T-47D meme kanseri hücrelerine farklı dozlarda uygulanan kurkuminin etkisi MTT yöntemi ve inverted mikroskop ile araştırılmıştır. Kurkuminin T-47D hücrelerinde IC50 dozu 24 saat sonunda 65,8 μM, 48 saat sonunda 46,4 μM ve 72 saat sonunda ise 26,6 μM olarak belirlenmiştir. Morfolojik değerlendirmede ise kurkumin uygulanmış hücreler yuvarlak ve flask yüzeyinden ayrılmış kitleler halinde gözlenmektedir. Sonuçlarımız, kurkuminin T-47D hücre proliferasyonunu önemli ölçüde azalttığını göstermektedir. Kurkumin, tek başına veya diğer moleküllerle kombinasyon halinde meme kanseri tedavisi için bir aday olabilir. Gelecekte, kurkuminin meme kanseri hücreleri üzerindeki etki mekanizmasını aydınlatmak için daha kapsamlı ve çok merkezli destekli ileri klinik çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

Kurkumin, Meme Kanseri, T-47D Meme Kanseri Hücreleri

Kaynakça

• Liu D, Chen Z. The effect of curcumin on breast cancer cells. J Breast Cancer. 2013;16(2):133-7.
• Hu S, Xu Y, Meng L, Huang L, Sun H. Curcumin inhibits proliferation and promotes apoptosis of breast cancer cells. Exp Ther Med. 2018;16(2):1266-72.
• Shen H, Shen J, Pan H, Xu L, Sheng H, Liu B, et al. Curcumin analog B14 has high bioavailability and enhances the effect of anti‐breast cancer cells in vitro and in vivo. Cancer Sci. 2021;112(2):815-27.
• Choudhuri T, Pal S, Agwarwal ML, Das T, Sa G. Curcumin induces apoptosis in human breast cancer cells through p53-dependent Bax induction. FEBS Lett. 2002;512(1-3):334-40.
• Fabianowska-Majewska K, Kaufman-Szymczyk A, Szymanska-Kolba A, Jakubik J, Majewski G, Lubecka K. Curcumin from Turmeric Rhizome: A Potential Modulator of DNA Methylation Machinery in Breast Cancer Inhibition. Nutrients. 2021;13(2):332.
• Patra S, Pradhan B, Nayak R, Behera C, Panda KC, Das S, et al. Apoptosis and autophagy modulating dietary phytochemicals in cancer therapeutics: Current evidences and future perspectives. Phytother Res. 2021.
• Khojaste E, Ahmadizadeh C. Catechin Metabolites along with Curcumin Inhibit Proliferation and Induce Apoptosis in Cervical Cancer Cells by Regulating VEGF Expression In-Vitro. Nutr Cancer. 2021:1-10.
• Bhatia M, Bhalerao M, Cruz‐Martins N, Kumar D. Curcumin and cancer biology: Focusing regulatory effects in different signalling pathways. Phytother Res. 2021.
• Giordano A, Tommonaro G. Curcumin and cancer. Nutrients. 2019;11(10):2376.
• Al-Ejeh F, Kumar R, Wiegmans A, Lakhani S, Brown M, Khanna K. Harnessing the complexity of DNA-damage response pathways to improve cancer treatment outcomes. Oncogene. 2010;29(46):6085-98.
• Udagawa T, Wood M. Tumor–stromal cell interactions and opportunities for therapeutic intervention. Curr Opin Pharmacol. 2010;10(4):369-74.
• Clusan L, Le Goff P, Flouriot G, Pakdel F. A closer look at estrogen receptor mutations in breast cancer and their implications for estrogen and antiestrogen responses. Int J Mol Sci. 2021;22(2):756.
• Kunnumakkara AB, Anand P, Aggarwal BB. Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins. Cancer Lett. 2008;269(2):199-225.
• Shakeri A, Ward N, Panahi Y, Sahebkar A. Anti-angiogenic activity of curcumin in cancer therapy: a narrative review. Curr Vasc Pharmacol. 2019;17(3):262-9.
• Rowe DL, Ozbay T, O'Regan RM, Nahta R. Modulation of the BRCA1 protein and induction of apoptosis in triple negative breast cancer cell lines by the polyphenolic compound curcumin. Breast cancer: basic and clinical research. 2009;3:BCBCR. S3067.
• Banerjee M, Singh P, Panda D. Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF‐7 cells. The FEBS journal. 2010;277(16):3437-48.
• Mehta K, Pantazis P, McQueen T, Aggarwal BB. Antiproliferative effect of curcumin (diferuloylmethane) against human breast tumor cell lines. Anticancer Drugs. 1997;8(5):470-81.
• Chiu T-L, Su C-C. Curcumin inhibits proliferation and migration by increasing the Bax to Bcl-2 ratio and decreasing NF-κBp65 expression in breast cancer MDA-MB-231 cells. Int J Mol Med. 2009;23(4):469-75.
• Nagaraju GP, Aliya S, Zafar SF, Basha R, Diaz R, El-Rayes BF. The impact of curcumin on breast cancer. Integrative Biology. 2012;4(9):996-1007.
• Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. Turmeric and curcumin: Biological actions and medicinal applications. Curr Sci. 2004:44-53.
• Pandya N, Khan E, Jain N, Satham L, Singh R, Makde RD, et al. Curcumin analogs exhibit anti-cancer activity by selectively targeting G-quadruplex forming c-myc promoter sequence. Biochimie. 2021;180:205-21.