Determination of the Effects of Helichrysum Plicatum DC. Subsp. Plicatum Ethanol Extract on Thallium Sulfate Induced Testicular Toxicity in Rat

Yıl 2024, Cilt: 17 Sayı: 1, 45 - 51, 30.04.2024

Ali Doğan Ömür , Betül Apaydın Yıldırım , Sefa Küçükler , Behzad Mokhtare , Özhan Karataş , Mustafa Özkaraca , Serkan Ali Akarsu

https://doi.org/10.52976/vansaglik.1385234

Öz

In this study, we aimed to determine the effects of the ethanol extract of Helichrysum plicatum DC plant on Thallium Sulfate (TS)-induced testicular toxicity in rats. For this purpose, a total of 24 Sprague Dawley rats, 6 each in four groups, were used in the study. Rats were administered a single dose of TS via IP and HPE via oral gavage for 8 days. After the administrations, the rats were sacrificed and blood and testicular tissues were collected. Testicular tissues were preserved for use in biochemical, histopathological, immunohistochemistry and in situ hybridization analyzes. The cauda epididymis was trimmed by separating from the testis and the resulting liquid was used for semen analysis. The sperm motility decreased and the rate of dead and abnormal spermatozoa increased in parallel to the increase (P<0.001) in oxidative damage in the TS group. Histopathological examination revealed degenerative and necrotic changes in the TS group. Cleaved caspase 3 gene expression decreased in the control group and TS group (P<0.05). The mean testicular biopsy score (MTBS) was the lowest in the TS group (P<0.05). There was a statistical difference between the groups in terms of immunohistochemical (IHC) and in situ hybridization (IST) parameters. In conclusion, it was observed that HPE could protect against TS-induced damage in testicular tissues of rats.

Anahtar Kelimeler

Helichrysum plicatum, oxidative stress, rat, sperm, thallium sulphate

Helichrysum Plicatum DC Subsp Plicatum Etanol Ekstraktının Sıçanlarda Talyum Sülfatın Neden Olduğu Testis Toksisitesi Üzerine Etkilerinin Belirlenmesi

Öz

Bu çalışmada, Helichrysum plicatum DC bitkisinin ratlarda Talyum Sülfat (TS) ile indüklenen testis toksisitesi üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Bu amaçla çalışmada her grupta 6 adet olmak üzere toplam 24 adet Sprague Dawley rat kullanıldı. Tedavilerin ardından ratlar sakrifiye edilerek kan ve testis dokuları alındı. Testis dokuları, biyokimyasal ve histopatolojik analizlerde kullanılmak üzere saklandı. Kauda epididim testisten ayrılarak traşlandı ve elde edilen sıvı semen analizi için kullanıldı. Çalışma bulgularımıza göre TS grubunda sperm motilitesinin azaldığı, ölü ve anormal spermatozoa oranının arttığı buna karşılık oksidatif hasarın arttığı (P<0.001) saptanmıştır. Histopatolojik incelemede TS grubunda nekrotik ve dejeneratif değişiklikler saptandı. Bölünmüş kaspaz 3 gen ekspresyonu kontrol grubu ve TS grubunda azaldı (P<0.05). Ortalama testis biyopsi skoru (MTBS) TS grubunda en düşüktü (P<0.05). Gruplar arasında immünohistokimyasal (IHC) ve in situ hibridizasyon (IST) parametreleri açısından istatistiksel fark vardı. Sonuç olarak, HPE'nin sıçanların testis dokularında TS kaynaklı hasara karşı koruma sağlayabildiği görülmüştür.

Anahtar Kelimeler

Helichrysum plicatum, oksidatif stres, sıçan, sperm, talyum sülfat.

Kaynakça

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