The Effect of Aglepriston on Some Hematological and Biochemical Parameters Used in Termination of Unwanted Pregnancies in Cats

Öz

In this study the effect of aglepristone in order to create abortion was investigated on some haematological and biochemical parameters on 22-30 days of pregnant cats. Twenty cats that their pregnancy is determined by ultrasonography were randomly divided into two groups. The experimental group (n:15) was formed by giving aglepristone at a dose of 15 mg/kg subcutaneously twice every 24 hours in the first group and the control group (n: 5) was formed by giving the same dose of 0.9% sodium chloride (NaCl) at the same time to the second group. All of the animals in both groups were examined as clinical every day. A complete blood count (CBC) on blood count device and biochemistry parameters Blood urea nitrogen (BUN), creatinine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), bilirubin, glucose on a Reflotron device were made every 2 days during 15 days. In our study it was observed that 14 of the 15 animals (94.4%) in the experimental group were aborted and 1 of them gave birth healthy at the end of the normal gestational period. In the biochemical analyzes although there were some statistical differences between the experimental and control groups it was observed that all values remained within the range of normal reference values. Consequently it was concluded that aglepristone can be used successfully for terminated of unwanted pregnancies on 22 - 30 days of pregnant cats and do not have negative effects on hematological and biochemical parameters.

Anahtar Kelimeler

• Aglepristone,Cat,Hematological,Biochemical,Parameter

Kedilerde İstenmeyen Gebeliklerin Sona Erdirilmesinde Kullanılan Aglepristonun Bazı Hematolojik ve Biyokimyasal Parametreler Üzerine Etkisi

Öz

Bu çalışmada 22-30 günlük gebe kedilerde abort oluşturmak amacıyla kullanılan aglepristonun bazı hematolojik ve biyokimyasal parametreler üzerine etkisi araştırıldı. Gebelikleri ultrasonografi ile belirlenen 20 adet kedi rastgele iki gruba ayrıldı 1. gruba (n:15) 24 saat ara ile iki kez 15mg/kg dozda aglepriston subcutan uygulanarak deney grubu, 2. gruba (n:5) aynı anda aynı dozda serum fizyolojik uygulanarak kontrol grubu oluşturuldu. Her iki gruptaki hayvanların tamamı her gün klinik muayeneden geçirildi. 15 gün boyunca 2 günde bir kan örnekleri alınarak kan sayım cihazında hemoglobin miktarı (Hb), hematokrit oranı (HCT), akyuvar sayısı (WBC) belirlendi. Kan üre azotu (BUN), kreatinin, Aspartat aminotransferaz (AST), Alanin aminotransferaz (ALT), bilirubin, glukoz ölçümleri Reflotron cihazında yapıldı. Çalışmamızda aglepriston uygulanan deney grubundaki 15 hayvandan 14 tanesinin abort yaptığı (% 94.4), 1 tanesinin normal gebelik süresi sonunda sağlıklı doğum yaptığı gözlendi. Yapılan biyokimyasal analizlerde deney ve kontrol grupları arasında istatistiksel açıdan bazı farklar bulunsada tüm değerlerin normal referans değerleri aralığında kaldığı gözlendi. Sonuç olarak aglepristonun 22-30 günlük gebe kedilerde istenmeyen gebeliklerin sona erdirilmesinde başarı ile kullanılabileceği, hematolojik ve biyokimyasal parametreler üzerinde olumsuz etki oluşturmadığı kanısına varıldı.

Anahtar Kelimeler

• Aglepriston,Kedi,Hematolojik,Biyokimyasal,Parametre

Kaynakça

1. Agaoglu AR, Schäfer-Somi S, Kaya D, Kucukaslan İ, Emre B, Gultiken N, Mulazımoglu BS, Colak A, Aslan S (2011). The intravaginal application of misoprostol improves induction of abortion with aglepristone Theriogenology, 76 (1), 74–82.Baan M, Taverne MA, Kooistra HS, de Gier J, Dieleman SJ, Okkens AC (2005). Induction of parturition in the bitch with the progesterone-receptor blocker aglépristone. Theriogenology, 63 (7), 1958–1972.Corrada Y, Garcia P, de la Sota PE, Huzman M, Landoni MF, Gobello C (2005). Decrease of body temperature after aglepristone treatment in bitches. Anim Reprod Sci, 87 (3-4) 295-299. Fieni F, Martal J, Marnet PG, Siliart B, Guittot F (2006). Clinical, biological and hormonal study of mid-pregnancy termination in cats with aglepristone Theriogenology, 66 (6-7), 1721–1728.Fieni F, Martal J, Marnet PG, Siliart B, Bernard F, Riou M, Bruyas PG, Tainturier D (2001). Hormonal variation in bitches after early or mid-pregnancy termination with aglepristone. J. Reprod Fertil, Suppl 57, 243-248.Galac S, Kooistra HS, Butinar J, Bevers MM, Dieleman SJ, Voorhout G, Okkens AC (2000). Termination of mid-gestation pregnancy in bitches with aglepristone, a progesterone receptor antagonist. Theriogenology, 53 (4), 941-950.Garcia Mitacek MC, Stornelli MC, Praderio R, Stornelli MA, de la Sota RL (2012). Efficacy of Cloprostenol or Aglepristone at 21–22 and 35–38 Days of Gestation for Pregnancy Termination in Queens. Reprod Dom Anim, 47 (6), 200–203. Georgiev P, Bostedt H, Goericke-Pesch S, Dimitrov M, Petkov P, Stojanthev K, Tsoneva V Wehrend A (2010). Induction of Abortion with Aglepristone in Cats on Day 45 and 46 After Mating. Reprod Dom Anim, 45, 161–167. Georgiev P, Wehrend A (2005). Mucometra in the cat-five cases. Tierartztl Prax, 33:112-114. Georgiev P, Wehrend A (2006). Mid-gestation pregnancy termination by the progesterone antagonist aglepristone in queens. Theriogenology, 65(7), 1401-1406.Goericke-Pesch S, Georgiev P, Wehrend A (2010). Prevention of pregnancy in cats using aglepristone on days 5 and 6 after mating. Theriogenology, 74 (2), 304–310.Goericke-Pesch S (2010). Reproduction Control in cats. J Feline Med Surg, 12, 539-546. Gogny A, Fieni F (2016). Aglepristone: A review on its clinical use in animals. Theriogenology, 85(4), 555–566.Gürbulak K, Pancarcı Ş M, Ekinci H, Konuk C, Kırşan I, Uçmak M Toydemir S (2005). Use of Aglepristone and Aglepristone + Intrauterine Antibiotic for the Treatment of Pyometra in Bitches. Acta Vet Hung, 53 (2), 249–255.İmren HY, Şahal M (1994). Veteriner İç Hastalıkları Medisan Yayınevi Ankara Jurka P, Max A (2009). Treatment of fibroadenomatosis in 14 cats with aglepristone-changes in blood parameters and follow-up. Vet Rec 165(22), 657-660 Musal B, Ağaoğlu AR (2013). Gebelik Fizyolojisi In: Köpek ve Kedilerde Doğum ve Jinekoloji, Kaymaz M, Fındık M, Rişvanlı A, Köker A (Ed), Medipres Matbaacılık Yayıncılık Ltd. Şti. Malatya Nak D, Nak Y, Tuna B (2009). Follow-up examinations after medical treatment of pyometra in cats with the progesterone-antagonist aglepristone. J Feline Med Surg, 11, 499-502.Oguejiofor CF, Ochiogu IS, Uchendu CN (2013). Changes in peripheral hormone levels after mid-gestation termination of pregnancy with aglepristone in rats Vet Arh 83 (1), 81-91.Özalp GR, Temizel EM, Özocak-Batmaz E (2013). Clinical, ultrasonography and haematology of aglepristone-induced mid-gestation pregnancy terminations in rabbits. J S Afr Vet Assoc, 84 (1) 998-1001.Özalp GR, Çalışkan Ç, Seyrek-Intaş K, Wehrend A (2010). Effects of the progesterone receptor antagonist aglepristone on implantation administered on days 6 and 7 after mating in rabbits. Reprod Domest Anim, 45 (3), 505–508.Özalp GR, Seyrek-Intaş K, Çalışkan Ç Wehrend A. (2008). Mid-gestation pregnancy termination in rabbits by the progesterone antagonist aglepristone. Theriogenology, 69 (9), 1056–1060.Schäfer-Somi S, Aksoy OA, Beceriklisoy HB, Einspanier A, Hoppen HO, Aslan S (2007). Repeated induction of abortion in bitches and the effect on plasma concentrations of relaxin, progesterone and estradiol-17beta. Theriogenology, 68 (6), 889–895.Turgut K (2000). Veteriner Klinik Laboratuvar Teşhis 2. Baskı Bahçıvanlar Basım San. A. Ş. Konya. Verstegen J (2000). Feline reproduction. In: Ettinger SJ, Feldman EC (eds) Texbook of Veterinary Internal Medicine: Diseases of the Dog and Cat (volume 1 and 2). Ed.5, WB Saunders, Philadelphia, 1585-1592.