Alt solunum yolu enfeksiyonu olan köpeklerde canine coronavirusun tespiti ve moleküler karakterizasyonu

Yıl 2022, Cilt: 93 Sayı: 2, 124 - 132, 15.06.2022

Fırat Doğan Serkan İrfan Köse

https://doi.org/10.33188/vetheder.1094617

Cited By: 1

Öz

Coronaviridae familyasında yer alan coronaviruslar insan ve hayvanlarda sindirim ve solunum sistemi enfeksiyonlarına neden olmaktadır. Alfacoronavirus içerisinde yer alan canine coronaviruslar (CCoV)’ın CCoV I ve CCoV II olmak üzere iki alt tipi bulunmaktadır. CCoV-II ise CCoV- IIa ve IIb olmak üzere iki genotipe ayrılmaktadır. CCoV her yaştaki ve her türlü beslenme şekline sahip köpekleri etkilemesine rağmen özellikle yeni doğan yavrular daha duyarlı ve ciddi şekilde etkilenebilmektedir. Yapılan literatür araştırmalarına göre ülkemizde özellikle alt solunum yolu enfeksiyonlarındaki canine coronavirus varlığının tespitine yönelik moleküler çalışmalara rastlanılmamıştır. Bu çalışmada alt solunum yolu enfeksiyonu tanımlanan barınak köpeklerinde CCoV’un tespiti ve moleküler karakterizasyonunun yapılması amaçlandı. Bu amaçla alt solunum yolu enfeksiyonu tespit edilen 40 adet barınak köpeğinden alınan Bronkoalveolar Lavaj (BAL) sıvıları incelendi. Test edilen 40 köpeğe ait BAL sıvılarından 3 tanesinde CCoV tespit edildi. Yapılan dizin analizi sonrasında elde edilen dizinler ile filogenetik ağaç yapıldı. Filogenetik ağaçta pozitif bulunan 3 örnekten 2 sinin CCoV-I, bir örneğin ise CCoV-II olduğu tespit edildi. Sonuç olarak bu çalışma ile barınak köpeklerinin alt solunum yolu rahatsızlıklarında CCoV-I ve CCoV-II’ nin rol oynayabileceği ortaya konulmuştur. Ayrıca aynı barınakta farklı hayvanlarda iki farklı CCoV’nun tespiti önemli bir veri olarak değerlendirilmiş olup özellikle barınak koşulları gibi kalabalık ortamda barındırılan köpeklerdeki her iki tipin tespiti gelecekte oluşabilecek yeni varyantların ya da alt tiplerin oluşabilme ihtimalinin de göz ardı edilmemesi gerektiğini göstermektedir.

Anahtar Kelimeler

Coronavirus, Köpek, PCR, Alt solunum sistemi enfeksiyonu

Detection and molecular characterization of canine coronavirus in dogs with lower respiratory tract infection

Öz

Coronaviruses in the family Coronaviridae cause digestive and respiratory system infections in humans and animals. There are two subtypes of canine coronaviruses (CCoV), which are included in the alfacoronavirus, as CCoV I and CCoV II. CCoV-II is divided into two genotypes, CCoV-IIa and IIb. Although CCoV affects dogs of all ages and all diets, newborn puppies can be particularly susceptible and severely affected. According to the literature research, no molecular studies have been found in our country for the detection of canine coronavirus, especially in lower respiratory tract infections. In this study, it was aimed to detect and molecular characterization of CCoV un shelter dogs with lower respiratory tract infection. For this purpose, Bronchoalveolar Lavage (BAL) fluids taken from 40 shelter dogs with lower respiratory tract infections were examined. CCoV was detected in 3 of the BAL fluids of 40 dogs tested. A phylogenetic tree was constructed with the sequences obtained after the sequence analysis. It was determined that 2 of the 3 positive samples in the phylogenetic tree were CCoV-I and one sample was CCoV-II. In conclusion, this study revealed that CCoV-I and CCoV-II may play a role in lower respiratory system disorders of shelter dogs. In addition, the detection of two different CCoVs in different animals in the same shelter has been considered as an important data, and the detection of both types in dogs housed in crowded environments such as shelter conditions shows that the possibility of new variants or subtypes that may occur in the future should not be ignored.

Anahtar Kelimeler

Coronavirus, Canine, PCR, Lower respiratory tract infection

Kaynakça

• Burrell C, Howard C, Murphy F. Fenner and White’s medical virology. 5th ed. United States: Academic Press. 2016.
• Decaro N, Buonavoglia C. An update on canine coronaviruses: viral evolution and pathobiology.Vet Mıcrobiol 2008;132(3-4):221-234.
• Decaro N, Mari V, Elia G, Addie DD, Camero M, Lucente MS, et al. Recombinant canine coronaviruses in dogs, Europe. Emerg Infect Dis 2010; 16(1):41.
• Pratelli A. Genetic evolution of canine coronavirus and recent advances in prophylaxis. Vet Res 2006;37(2):191-200.
• Herrewegh AA, Smeenk I, Horzinek MC, Rottier PJ, De Groot RJ. Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus. J Virol 1998;72(5):4508-4514.
• Decaro N, Buonavoglia C. Canine coronavirus: not only an enteric pathogen. Vet Clin N Am-Small 2011;41(6):1121-1132.
• Erles K, Brownlie J. Sequence analysis of divergent canine coronavirus strains present in a UK dog population. Virus Res 2009;141(1):21-25.
• Ntafis V, Mari V, Decaro N, Papanastassopoulou M, Pardali D, Rallis TS, et al. Canine coronavirus, Greece. Molecular analysis and genetic diversity characterization. Infect Genet Evol 2013;16: 129-136.
• Van Der Hoek L, Pyrc K, Jebbink MF, Vermeulen-Oost W, Berkhout RJ, Wolthers KC, et al. Identification of a new human coronavirus. Nat Med 2004;10(4):368-373.
• Storz J, Lin X, Purdy CW, Chouljenko VN, Kousoulas KG, Enright FM, et al. Coronavirus and Pasteurella infections in bovine shipping fever pneumonia and Evans' criteria for causation. J Clin Microbiol 2000;38(9): 3291-3298.
• Erles K, Dubovi EJ, Brooks HW, Brownlie J. Longitudinal study of viruses associated with canine infectious respiratory disease. J Clin Microbiol 2004; 42(10):4524-4529.
• Makela MJ, Puhakka T, Ruuskanen O, Leinonen M, Saikku P, Kimpimäki M, et al. Viruses and bacteria in the etiology of the common cold. J Clin Microbiol 1998; 36(2): 539-542.
• Erles K, Brownlie J. Investigation into the causes of canine infectious respiratory disease: antibody responses to canine respiratory coronavirus and canine herpesvirus in two kennelled dog populations. Arch Viro 2005; 150(8):1493-1504.
• Akkutay-Yoldar ZA, Koç BT, Oguzoglu TC. Phylogenetic analysis of partial transmembrane protein gene of canine coronaviruses detected in Turkey. Ankara Unıv Vet Fak 2020;67(3):265-271.
• Timurkan MO, Aydin H, Dincer E, Coskun N. Molecular characterization of canine coronaviruses: an enteric and pantropic approach. Arch Viro 2021;166(1):35-42.
• Avci O, Bulut O, Yapici O, Hasircioglu S, Simsek A. Canine coronavirus infection in dogs in Turkey: Virological and serological evidence. Indian J Anım Res 2016;50(4):565-568.
• Gür S, Gençay A, Doğan N. A serologic investigation for canine corona virus infection in individually reared dogs in central Anatolia. Erciyes Üni Vet Fak Derg 2008;5(2):67-71.
• Yeşilbag K, Yilmaz Z, Torun S, Pratelli A. Canine coronavirus infection in Turkish dog population. J Vet Med, Series B 2004;51(7): 353-355.
• Ataseven VS, Ucar H, Akca Y. Canine coronavirus antibodies in stray dogs. Indian Vet J 2005; 82(7):782-783.
• Pratelli A, Tempesta M, Greco G, Martella V, Buonavoglia C. Development of a nested PCR assay for the detection of canine coronavirus. J Virol Methods 1999;80(1):11-15.
• Larsson A. AliView: A fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 2014;30: 3276–3278.
• Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25(17):3389-3402.
• Weese JS, Stull J. Respiratory disease outbreak in a veterinary hospital associated with canine parainfluenza virus infection. The Canadian Vet J 2013;54(1):79.
• Köse Sİ, Maden M, Sayin Z. Clinical and bacteriological analysis of respiratory tract infections in sheltered dogs and determination of antibacterial treatment options. J Hellenic Vet Med Society 2021;72(4):3491-3502.
• Radtanakatikanon A, Keawcharoen J, Taya Charoenvisal N, Poovorawan Y, Prompetchara E, et al. Genotypic lineages and restriction fragment length polymorphism of canine distemper virus isolates in Thailand. Vet Microbiol 2013;166(1-2):76-83.
• Tham KM, Homer GW, Hunter R. Isolation and identification of canine adenovirus type-2 from the upper respiratory tract of a dog. New Zeal J 1998; 46(3):102-105.
• Erles K, Toomey C, Brooks HW, Brownlie J. Detection of a group 2 coronavirus in dogs with canine infectious respiratory disease. Virology 2003; 310(2):216-223.
• Posuwan N, Payungporn S, Thontiravong A, Kitikoon P, Amonsin A, Poovorawan Y. Prevalence of respiratory viruses isolated from dogs in Thailand during 2008–2009. Asian Biomed 2010;4(4):563-69.
• Pratelli A, Tinelli A, Decaro N, Camero M, Elia G, Gentile A, et al. PCR assay for the detection and the identification of atypical canine coronavirus in dogs. J Virol Methods 2002a;106(2):209-213.
• Pratelli A, Decaro N, Tinelli A, Martella V, Elia G, Tempesta M, et al. Two genotypes of canine coronavirus simultaneously detected in the fecal samples of dogs with diarrhea. J Clin Microbiol 2004;42(4):1797-1799.
• Buonavoglia C, Decaro N, Martella V, Elia G, Campolo M, Desario C, et al. Canine coronavirus highly pathogenic for dogs. Emerg Infect Dis 2006;12(3):492.
• Sedlak RH, Jerome KR. Viral diagnostics in the era of digital polymerase chain reaction. Diagn Micr Infec Dis 2013;75(1): 1-4.
• Huggett JF, Cowen S, Foy CA. Considerations for digital PCR as an accurate molecular diagnostic tool. Clin Chem 2015;61(1):79-88.
• Woo PC, Lau SK, Huang Y, Yuen KY. Coronavirus diversity, phylogeny and interspecies jumping. Exp Biol Med 2009;234(10):1117-1127.
• Dagalp SB, Sahinkesen I, Babaoglu AR, Dogan F, Arslan EA. Animal coronaviruses, interspecies transmission and zoonotic potential.(Covid-19 special ıssue) Eurasian J Vet Sci 2020;99-105.
• Pratelli A, Elia G, Martella V, Tineili A, Decaro N, Marsilio F, et al. M gene evolution of canine coronavirus in naturally infected dogs. Vet Rec 2002b; 151(25):758-761.
• Pratelli A, Martella V, Decaro N, Tinelli A, Camero M, Cirone F, et al. Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy. J Virol Methods 2003a; 110(1): 9-17.
• Pratelli A, Martella V, Pistello M, Elia G, Decaro N, Buonavoglia D, et al. Identification of coronaviruses in dogs that segregate separately from the canine coronavirus genotype. J Virol Methods 2003b; 107(2): 213-222.