Cytotoxic and antiproliferative effects of hellebrin on breast and lung cancer cells

Yıl 2023, Cilt: 94 Sayı: 2, 137 - 143, 15.06.2023

Yaşar Şahin , Mustafa Türk , Sedat Sevin , Kevser Peker , Esra Bozkaya , Seydi Ali Peker , Aleyna Çavdar

https://doi.org/10.33188/vetheder.1254469

Öz

This study aimed to investigate the impact of hellebrin on human lung cancer cell (H1299) and breast cancer cell (MCF-7) lines over time. The viability of various concentrations of hellebrin (100 nM-400 nM) on two cancer cell lines was measured using the MTT method. The cellular proliferation over time was examined using xCELLigence real-time cell analysis (RTCA). The results showed a significant decrease in cell viability with increasing concentrations of hellebrin in both cancer cell lines compared to the control group (p˂0.0001). While the viability of both cancer cells decreased after a certain period of hellebrin application, the viability of the control groups increased over time. These findings indicate that hellebrin has high antiproliferative and cytotoxic effects on breast and lung cancer cells. Consequently, this study suggests that hellebrin may have potential as a treatment for other cancers, especially lung cancer, and further in vitro and in vivo experiments are needed to investigate this possibility.

Anahtar Kelimeler

Breast cancer cell, Cytotoxicity, Hellebrin, Lung cancer cell, xCELLigence system

Hellebrin'in meme ve akciğer kanseri hücreleri üzerinde sitotoksik ve antiproliferatif etkileri

Öz

Bu çalışmada, hellebrinin insan akciğer kanseri hücresi (H1299) ve meme kanseri hücresi (MCF-7) hatları üzerindeki etkisinin zaman içinde araştırılması amaçlanmıştır. Hellebrin'in (100 nM-400 nM) çeşitli konsantrasyonlarının iki kanser hücre hattı üzerindeki canlılığı, MTT yöntemi kullanılarak ölçüldü. Zaman bağlı hücresel çoğalma, xCELLigence gerçek zamanlı hücre analizi (RTCA) kullanılarak incelenmiştir. Sonuçlar, kontrol grubuna kıyasla her iki kanser hücre hattında artan hellebrin konsantrasyonları ile hücre canlılığında önemli bir düşüş gösterdi (p˂0.0001). Belli bir süre hellebrin uygulamasından sonra her iki kanser hücresinin canlılığı azalırken, kontrol gruplarının canlılığı zamanla arttı. Bu bulgular, hellebrin'in meme ve akciğer kanseri hücreleri üzerinde yüksek antiproliferatif ve sitotoksik etkilere sahip olduğunu göstermektedir. Sonuç olarak, bu çalışma hellebrin'in diğer kanserler, özellikle akciğer kanseri için bir tedavi potansiyeline sahip olabileceğini ve bu olasılığı araştırmak için daha fazla in vitro ve in vivo deneylere ihtiyaç olduğunu düşünülmektedir.

Anahtar Kelimeler

Akciğer kanseri hücresi, Hellebrin, Meme kanseri hücresi, Sitotoksisite, xCELLigence sistemi

Kaynakça

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