Pirinç Biti Sitophilus oryzae Popülasyonlarında Wolbachia’nın Belirlenmesi

Yıl 2019, Cilt: 4 Sayı: 1, 23 - 31, 20.12.2019

Kübra Türkmen Erhan Koçak

Öz

Özet 1 :Sitophilus oryzae
(Coleoptera: Curculionidae ) başta mısır olmak üzere tüm hububat çeşitlerinde
zararlıdır. Hububat danelerinde beslenmeleri sonucunda üründe kalite ve ağırlık
yönünden kayıplar meydana getirir. Endosimbiyont bakteri olan Wolbachia,
eklembacaklıların %66’sında tespit edilmiş olup konukçularının üremesi üzerinde
partenogenesis, erkek öldürücülük, feminizasyon ve sitoplazmik uyumsuzluk
şeklinde üreme bozukluğuna neden olan önemli bir bakteridir. Çalışmada Ege
(İzmir, Manisa), İç Anadolu (Ankara, Konya), Karadeniz (Samsun), Güneydoğu
Anadolu (Diyarbakır, Şanlıurfa) ve Akdeniz (Adana) bölgelerinden toplam 12
lokasyondan S. oryzae popülasyonları toplanmış ve moleküler yöntemler
ile Wolbachia varlığı araştırılmıştır. Çalışmada DNA ekstrasyonunu
takiben PCR reaksiyonları wsp primerleriyle taranmıştır. Jel görüntülerine
göre, taranan 120 örnekten 118 (%98,33)’inde Wolbachia tespit
edilmiştir. Şanlıurfa ve Adana (Çukurköprü)’da %90 oranında, Manisa, İzmir,
Ankara (Haymana-Karakeçeli), Diyarbakır, Samsun, Konya (Merkez-Alibeyköy) ve
Adana (Ceyhan) illerinde ise %100 oranında Wolbachia varlığı tespit
edilmiştir. Bu çalışma ülkemizde S. oryzae üzerinde Wolbachia
varlığının belirlendiği ilk çalışmadır.

Özet 2 : Sitophilus
oryzae
(Coleoptera: Curculionidae ) is the harmful insect for grains and especially
for corn; as a result of consuming grains, it leads to quality and weight loss
in products. Wolbachia, an
endosimbiyont bacteria, has been found in %66 of arthropods, and causes various
reproduction disorders including partenogenesis, male lethality, feminisation
and cytoplasmic incompatibility. In the present study, S. oryzae populations were collected from 12 locations in Agean region
(Izmir and Manisa), Central Anatolia region (Ankara and Konya), Black Sea region
(Samsun), South-eastern Anatolia region (Diyarbakir and Şanlıurfa) and
Mediterranean region (Adana). Existence of Wolbachia
was investigated through molecular methods. Specifically the PCR reactions following
DNA extraction were scanned with Wsp
primers. According to gel displays, Wolbachia
was determined in 118 out of 120 samples. In particular, the samples in
Şanlıurfa and Adana (Cukurkopru) contained Wolbachia
as 90%, and the samples in Manisa, Izmir, Ankara (Haymana-Karakeceli),
Diyarbakir, Samsun, Konya (Merkez-Alibeyköy) and Adana (Ceyhan) infected Wolbachia as 100%. The study is the
first study on determination of Wolbachia
in S. oryzae populations in Turkey.

Anahtar Kelimeler

Wolbachia, Endosimbiyont, wsp, Sitophilus oryzae

Kaynakça

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