Effects of Rosmarinus officinalis Essential Oil Supplimentation on Growth Performance and Some Blood Parameters in Rainbow Trout (Oncorhynchus mykiss, Walbaum 1792)

Abstract

The aim of this study was to determine the effects of rosemary(Rosmarinus officinalis) oil on growth performance, hematological and  biochemical  parameters in rainbow trout. For this purpose,  300 healthy rainbow trout (10,14±0,06 g) were divided into four groups (75 fish in each group) and fed diets supplemented with  0% (control), 0.025 %, 0.1 % and 0.05 %  rosemary (Rosmarinus officinalis) oil for 60 days. As a result of our study, the addition of rosemary oil to feed did not have positive effects on growth and feed intake. In addition, rosemary oil addition to feed did not cause a change in total erythrocyte count, hemoglobin concentration, glucose, triglyceride, total protein and creatinine (P>0.05).However,  white blood cells counts (total  leukocytes) was significantly increased in fish fed with 0.025 % and 5 % rosemary oil (p<0.05). Furthermore, in all groups except the control group, there was a significant decrease in the levels of Na + and Cl-(p<0.05).

Keywords

• Rainbow trout,rosemary oil,growth,hematologic profile

Gökkuşağı Alabalığı (Oncorhynchus mykiss, Walbaum 1792)’nın Yemlerine Biberiye Bitki (Rosmarinus Officinalis) Yağı İlavesinin Balıkların Büyüme Performansı ve Bazı Kan Parametrelerine Olan Etkileri

Öz

Bu çalışma ile, biberiye (Rosmarinus officinalis) yağının gökkuşağı alabalıklarında gelişim performansı, hematolojik ve biyokimyasal parametreler üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Bu amaçla, toplam 300 adet sağlıklı gökkuşağı alabalığı(10,14±0,06 g) her bir grupta 75 balık olacak şekilde dört gruba bölünmüş ve balıklar 60 gün süreyle, %0(kontrol) ve  %0,025, 0,1 ve 0,05 biberiye yağı ilave edilen yemlerle beslenmiştir. Araştırmamız sonucunda, yeme biberiye yağı ilavesinin gelişim ve yem alımı üzerinde olumlu etkisi bulunmamıştır. İlave olarak, yeme biberiye yağı ilavesi total eritrosit sayısı, hemoglobin konsantrasyonu, glikoz, trigliserid, toplam protein ve keratinin seviyelerinde bir değişikliğe sebep olmamıştır. Bununla birlikte, yemlerine %0,025 ve %5 oranında biberiye yağı ilave edilen balıklarda beyaz kan hücresi (total lökosit) sayısı önemli derecede atmıştır (p<0,05). Ayrıca, kontrol grubu hariç bütün gruplarda kandaki Na+ve Cl- seviyeleri önemli ölçüde düşmüştür (p<0,05).

Anahtar Kelimeler

• gökkuşağı alabalığı,biberiye yağı,gelişim,hematolojik profil

Kaynakça

1. Aly, S.M., Mohamed, M.F., 2010. Echinacea purpurea and Allium sativum as immunostimulants in fish culture using Nile tilapia (Oreochromis niloticus). Journal of Animal Physiology and Animal Nutrition 94, 31–39.
2. Aly, S.M., Atti, N.M.A., Mohamed, M.F., 2008. Effect of Garlic on the Survival, Growth, Resistance and Quality of Oreochromis niloticus. Proceedings of the 8th International Symposium on Tilapia in Aquaculture, pp. 277–296.
3. Awad, E., Austin, B., 2010. Use of lupin, Lupinus perennis, mango, Mangifera indica, and stinging nettle, Urtica dioica, as feed additives to prevent Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 33, 413–420.
4. Bakırel, T., Bakırel, U., Keleş, O. Ü., Ülgen, S. G., Yardibi, H., 2008. In vivo assessment of antidiabetic and antioxidant activities of rosemary (Rosmarinus officinalis) in alloxan-diabetic rabbits. Journal of Ethnopharmacology, 116(1), 64-73.
5. Bulfon, C., Volpatti, D., ve Galeotti, M., 2015. Current research on the use of plant‐derived products in farmed fish. Aquaculture Research, 46(3), 513-551.
6. Di Turi, L, Ragni, M, Caputi Jambrengh,i A, Lastilla, M, Vicenti, A, Colonna, MA, Giannico, F, Vonghia, G, 2009. Effect of dietary rosemary oil on growth performance and flesh quality of farmed seabass (Dicentrarchus labrax). Ital J Anim Sci, 8:857–859.
7. Goda, A.M.A.S., 2008. Effect of dietary ginseng herb (Ginsana ® G115) supplementation on growth, feed utilization, and hematological indices of Nile tilapia, Oreochromis niloticus(L.) fingerlings. Journal of the World Aquaculture Society, 39, 205–214.
8. Gültepe, N., Bilen, S., Yılmaz, S., Güroy, D., Aydın, S., 2014. Effects of herbs and spice on health status of tilapia (Oreochromis mossambicus) challenged with Streptococcus iniae. Acta Veterinaria Brno, 83(2), 125-131.

9. Harikrishnan R., Balasundaram C., Heo M.S., 2010. Herbal supplementation diets on haematology and innate immunity in goldfish against Aeromonas hydrophila. Fish and Shellfish Immunology, 28, 354–361.
10. Hernández, A., García, B. G., Caballero, M. J., Hernández, M.D., 2015. Preliminary insights into the incorporation of rosemary extract (Rosmarinus officinalis L.) in fish feed: influence on performance and physiology of gilthead seabream (Sparus aurata). Fish Physiology and Biochemistry, 41(4), 1065-1074.
11. Hwang, P.P., Lee, T.H., 2007. New insights into fish ion regulation and mitochondrion-rich cells. Comp. Biochem. Physiol., 48, 479-497.
12. Immanuel, G., Uma R.P., Iyapparaj P., Citarasu T., Punitha P.S.M., Michael B.M., Palavesam A., 2009. Dietary medicinal plant extracts improve growth, immune activity and survival of tilapia Oreochromis mossambicus. Journal of Fish Biology, 74, 1462–1475.
13. Jian, J., Wu Z., 2004. Influences of traditional Chinese medicine on non-specific immunity of Jian carp (Cyprinus carpio var. Jian). Fish and Shellfish Immunology, 16, 185–191.
14. Ji, S.C., Jeong G.-S., Im G.S., Lee S.W., Yoo J.H. & Takii K. (2007a) Dietary medicinal herbs improve growth performance, fatty acid utilization, and stress recovery of Japanese flounder. Fisheries Science 73, 70–76.
15. Ji, S.C., Takaoka O., Jeong G.S., Lee S.W., Ishimaru K., Seoka M., Takii K., 2007b. Dietary medicinal herbs improve growth and some non-specific immunity of red sea bream Pagrus major. Fisheries Science 73, 63–69.
16. Kaleeswaran, B., Ilavenil, S., Ravikumar, S., 2012. Changes in biochemical, histological and specific immune parameters in Catla catla (Ham.) by Cynodon dactylon (L.). Journal of King Saud University-Science, 24(2), 139-152.
17. Kamalakkannan, N, Prince P.S.M., 2006. Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin-induced diabetic wistar rats. Basic Clin Pharmacol Toxicol, 98:97–103.
18. Mathan, R., Senthil Kumar, K., Mahalashmi, P., 2010. Alterations in plasma electrolyte levels of a freshwater fish. Cyprinus carpio exposed to acidic pH. Toxicol. Environ. Chem., 92, 149-157.
19. McCarty, L.S., Houston, A.H., 1976. Effects of exposure to sublethal levels of cadmium upon water electrolyte status in the gold fish, Carassius auratus. J. Fish Biol., 9, 11-19.
20. Mohamad, S., Abasali, H., 2010. Effect of plant extracts supplemented diets on immunity and resistance to Aeromonas hydrophila in common carp (Cyprinus carpio). Agricultural Journal, 5, 119–127.
21. Nya, E.J., Austin, B., 2009a. Use of garlic, Allium sativum, to control Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 32, 963–970.
22. Nya, E.J., Austin, B., 2009b. Use of dietary ginger, Zingiber officinale Roscoe, as an immunostimulant to control Aeromonas hydrophila infections in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 32, 971–977.
23. Pratheepa, V., Ramesh, S., Sukumaran, N., 2010. Immunomodulatory effect of Aegle marmelos leaf extract on freshwater fish Cyprinus carpio infected by bacterial pathogen Aeromonas hydrophila. Pharmaceutical Biology, 48, 1224–1239.
24. Punitha, S.M.J., Babu, M.M., Sivaram, V., Shankar, V.S., Dhas, S.A., Mahesh, T.C., Immanuel, G., Citarasu, T., 2008. Immunostimulating influence of herbal biomedicines on non-specific immunity in grouper Epinephelus tauvina juvenile against Vibrio harveyi infection. Aquaculture International, 16, 511–523.
25. Reverter, M., Bontemps, N., Lecchini, D., Banaigs, B., Sasal, P., 2014. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture, 433, 50-61.
26. Sahu, S., Da,s B.K., Mishra, B.K., Pradhan, J., Sarangi, N., 2007. Effect of Allium sativum on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. Journal of Applied Ichthyology, 23, 80–86.
27. Sahu, S., Das, B.K., Mishra, B.K., Pradhan, J., Samal, S.K., Sarangi, N., 2008. Effect of dietary Curcuma longa on enzymatic and immunological profiles of rohu, Labeo rohita (Ham.), infected with Aeromonas hydrophila. Aquaculture Research, 39, 1720–1730.
28. Saravanan, M., Ramesh, M., Malarvizhi, A., Petkam, R., 2011. Toxicity of neem leaf extracts (Azadirachta indica A. Juss) on some haematological, ionoregulatory, biochemical and enzymological parameters of Indian major carp, Cirrhinus mrigala. Journal of Tropical Forestry and Environment, 1(1), 14-26.
29. Shalaby, A.M., Khattab, Y.A., Abdel Rahman, A.M., 2006. Effects of garlic (Allium sativum) and chloramphenicol on growth performance, physiological parameters and survival of Nile tilapia (Oreochromis niloticus). Journal of Venomous Animals and Toxins Including Tropical Diseases, 12, 172–201.
30. Suvetha, L., Ramesh, M., Saravanan, M., 2010. Influence of cypermethrin toxicity on ionic regulationand gill Na+/K+-ATPase activity of a freshwater teleost fish Cyprinus carpio. Environ. Toxicol. Pharmacol., 29, 44-49
31. Şengül, H., 2007. Manan-Oligosakkarit (Mos) Kullanımının Sazan (Cyprinus Carpio) Yavrularının Büyüme, Karaciğer Ve Barsak Histolojisine Etkisi. Yüksek Lisans Tezi. Mustafa Kemal Üniversitesi, Antakya.
32. Thomas, P.C., Murthy, T.C., 1976. Studies on the impact of a ferric organic pesticide on certain fish enzymes. Indian J. Animal Sci., 46, 619-624.
33. Toffaletti, J.G., McDonnell, E.H., 2008. Variation of serum creatinine, cystatin C and creatinine clearance tests in persons with normal renal function, Clinica Chimica Acta, 395, 115–119.
34. Turan, F., Gürağaç, R., ve Sayın, S. (2012). Su ürünleri yetiştiriciliğinde esansiyel yağlar. Türk Bilimsel Derlemeler Dergisi, 5(1), 35-40.
35. Van, Hai, N., 2015. The use of medicinal plants as immunostimulants in aquaculture: A review. Aquaculture, 446, 88-96.
36. Vasudeva, R.Y., Das, B.K., Jyotyrmayee, P., Chakrabarti, R., 2006. Effect of Achyranthes aspera on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. Fish and Shellfish Immunology, 20, 263–273.
37. Wood, C.M., Hogstrand, C., Glavez, F., Munger, R.S., 1996. The physiology of waterborne silver toxicity in freshwater rainbow trout, Oncorhynchus mykiss. The effects of silver thiosulfate. Aquat. Toxicol., 35, 111-125.
38. Xie, J., Liu, B., Zhou, Q., Su, Y., He Y., Pan L., Ge X. & Xu P., 2008. Effects of anthraquinone extract from rhubarb Rheum officinale bail on the crowding stres response and growth of common carp Cyprinus carpio var. Jian. Aquaculture, 281, 5–11.
39. Yılmaz, S, Ergun, S, Celik, E.S.¸ 2012. Effects of herbal supplements on growth performance of sea bass (Dicentrarchus labrax): change in body composition and some blood parameters. J Bio Sci Biotech, 1:217–222
40. Zheng, Z.I., Tan, J.Y.W., Liu, H.Y., Zhou, X.H., Xiang, X., Wang, K.Y., 2009. Evaluation of oregano essential oil (Origanum heracleoticum L.) on growth, antioxidant effect and resistance against Aeromonas hydrophila in channel catfish (Ictalurus punctatus). Aquaculture, 292, 214–218.