Bazı Ticari Lipazların Polietilenimin ile Kompleks Oluşturma Özelliklerinin Araştırılması

Yıl 2024, , 189 - 199, 30.04.2024

Eda Ondul Koc , Mert Yılmaz

https://doi.org/10.53433/yyufbed.1319182

Öz

Lipazlar, çok sayıda endüstriyel proseste kullanılan enzimlerdir ve biyokatalizör olarak uygulanabilirliklerini artırmak için immobilize edilmektedirler. Bu çalışmada, Novozyme 51032 (Fusarium solani pisi), Palatase 20000 L (Rhizomucor miehei), Lipolase 100 L (Thermomyces lanuginosus), Lipozyme CAL B L (Candida antarctica B) ve Amano (Pseudomonas fluorescens) kaynaklı ticari enzimlerin, polietilenimin (PEI) ile kompleks ve agregat oluşturması incelenmiştir. Enzimlerin, polietilenimin ile en iyi kompleks oluşturduğu PEI/enzim oranının; Novozyme 51032, Palatase 20000 L ve Lipolase 100 L için 1/20-80 aralığında olduğu görülmüştür. Lipozyme CAL B L ve (Amano) P. fluorescens, PEI ile agregat oluşturamamıştır. Bu çalışma; bazı ticari enzimlerin, PEI ile agregat oluşturmasını engelleyen çeşitli safsızlıklar içerebileceğini göstermiştir. Polietileniminin-enzim kompleksi, katyonik bir polimer olan PEI’ nin, enzimlerle elektrostatik etkileşimi esasına dayanmaktadır.

Anahtar Kelimeler

Biyokatalizör, Lipaz, PEI-lipaz agregatı, Polietilenimin

Destekleyen Kurum

Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Proje Merkezi

Proje Numarası

2015-MIM-B110

Teşekkür

Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Proje Merkezi'ne desteklerinden dolayı teşekkürü bir borç biliriz..

Investigation of Complexing Properties with Polyethyleneimine of Some Commercial Lipases

Öz

Lipases are enzymes used in various industrial process and are immobilized to increase their applicability as biocatalysts. Ionic polymers such as polyethyleneimine (PEI) make possible the co-precipitation of enzymes. In this study, complexation and aggregation with PEI of enzymes were investigated with commercial enzymes from Novozyme 51032 (Fusarium solani pisi), Palatase 20000 L (Rhizomucor miehei), Lipolase 100 L (Thermomyces lanuginosus), Lipozyme CAL B L (Candida antarctica B) and Amano (Pseudomonas fluorescens) using PEI as a linker and aggregation agent. The highest percentage of PEI-enzyme agregate was obtained for Novozyme 51032, Palatase 20000 L and Lipolase 100 L at the PEI/enzyme ratio of a 1/20-1/80 range. This study documented that Lipozyme CAL B L and (Amano) P. fluorescens enzyme preparations failed to occur precipitates resulting PEI-enzyme aggregates. The some commercial lipase preparations may contain various impurity components that prevent complexation or aggregation with PEI. Complexing with PEI of lipases is based on of basis electrostatic interaction of enzyme with PEI as a cationic polymer resulting in PEI-lipase aggregates.

Anahtar Kelimeler

Biocatalyst, Lipase, PEI-lipase agregate, Polyethyleneimine

Proje Numarası

2015-MIM-B110

Kaynakça

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