Investigation of Androgenesesis Capacity of Rwandan Pili-Pili Variety (Capsicum chinense L.) in Turkey

Yıl 2019, Cilt: 24 Sayı: 3, 170 - 175, 31.12.2019

Flavien Shımıra Serhat Yıldız Gökhan Baktemur Davut Keleş Mehmet Zahit Aydın , Saadet Büyükalaca Hatıra Taşkın

Öz

In this study, anther culture performance of Rwandan Pili-Pili variety was assessed in the Mediterranean Region of Turkey and compared to two Turkish C. annuum controls having low (A111 Kahramanmaraş type-green type) and high (Inan 3363 variety- Urfa type) androgenesis capacity, respectively. The anthers sampled were cultured in Murashige and Skoog (MS) medium including 30 g L^-1 sucrose, 2.5 g L^-1 activated charcoal, 15 mg L^-1 silver nitrate (AgNO₃), 4 mg L^-1 1-Naphthaleneacetic acid (NAA), 0.5 mg L^-1 6-Benzylaminopurine (BAP), 6.5 g L^-1 agar in the first stage. The anthers were then transferred to MS medium containing 30 g L^-1 sucrose and 6.5 g L^-1 agar. The embryos obtained were cultured in the same nutrient medium. At the end of the study, 19.4 embryos per 100 anthers were obtained from the control Inan 3363 and 4.46 from A111. As expected, Inan 3363 showed better performance than A111 genotype. In spite of all efforts, no embryo could be obtained in the Pili-Pili variety, although proper anther culture stage was especially investigated for this Rwandan variety. Cytological investigations showed that classical pepper anther culture morphological markers did not work in Rwandan Pili-Pili variety. In order to ascertain that the anther culture response of this variety is weak, other trials should be repeated in Rwanda. If there is no response in Rwanda, it could be concluded that this variety does not respond to anther culture.

Anahtar Kelimeler

Capsicum chinense, pepper, anther culture, Rwanda

Proje Numarası

FBA-2017-7761

Kaynakça

• Al Remi, F., Taşkin, H., Sönmez, K., Büyükalaca, S., & Ellialtioğlu, Ş. (2014). Effect of genotype and nutrient medium on anther culture of pepper (Capsicum annuum L). Turkish Journal of Agricultural and Natural Sciences, 1, 108-116.
• Arı, E., Yıldırım, T., Mutlu, N., Büyükalaca, S., Gökmen, Ü., & Akman, E. (2016). Comparison of different androgenesis protocols for doubled haploid plant production in ornamental pepper (Capsicum annuum L.). Turk. J. Biol., 40, 944-954. doi:10.3906/biy-1509-36Ata, A., Keleş, D., Taşkın, H., & Büyükalaca, S., (2019). Effects of season, genotype, and nutrient medium on pepper anther culture and microspore development. Turk. J. Agric. For., 43, 123-137. doi:10.3906/tar-1802-35.
• Buyukalaca, S., Comlekcioglu, N., Abak, K., Ekbic, E., & Kilic, N. (2004). Effect of silver nitrate and donor plant growing conditions on production of pepper (Capsicum annuum L.) haploid embryos via anther culture. Eur. J. Hortic. Sci., 69, 206-209.
• Denli (2019). Expansion of genetic basis via interspecies crossing of pepper (Capsicum annuum) and determination the inheritance of androgenesis. (PhD), Cukurova University, Institute of Natural and Applied Sciences, Adana, Turkey.
• Grozeva, S., Todorova, V., Cholakov, T., & Rodeva, V. (2013). Effect of temperature and growth period of donor plants on pepper anther culture. 3rd International Conference: Research People and Actual Tasks on Multidisciplinary Sciences, Lozenec, Bulgaria.
• Irikova, T., Grozeva, S., & Rodeva, V. (2011). Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiol. Plant, 33, 1559-1570. doi: 10.1007/s11738-011-0736-6
• Keleş, D., Pınar, H., Ata, A., Taşkın, H., Yıldız, S., & Büyükalaca, S. (2015). Effect of pepper types on obtaining spontaneous doubled haploid plants via anther culture. HortScience, 50, 1-6. doi: https://doi.org/10.21273/HORTSCI.50.11.1671
• Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant., 15, 473-497.
• Niklas-Nowak, A., Olszewska, D., Kisiala, A., & Nowaczyk, P. (2012). Study of individual plant responsiveness in anther cultures of selected pepper (Capsicum spp.) genotypes. Folia Horticulturae, 24, 141-146. doi: https://doi.org/10.2478/v10245-012-0017-x
• Nowaczyk, P., Olszewska, D., & Kisiala, A. (2009). Individual reaction of Capsicum F2 hybrid genotypes in anther cultures. Euphytica, 168, 225-233. doi: 10.1007/s10681-009-9909-4
• Olszewska, D., Kisiala, A., Niklas-Nowak, A., & Nowaczyk, P. (2014). Study of in vitro anther culture in selected genotypes of genus Capsicum. Turk. J. Biol., 38, 118-124. doi:10.3906/biy-1307-50
• Ozsan, T., & Onus, N. (2017). In vitro pepper (Capsicum annuum L.) anther culture: can be affected via vitamins B? Biotech. J. Int. 20, 1-13. doi: 10.9734/BJI/2017/37102
• Taşkin, H., Büyükalaca, S., Keleş, D., & Ekbiç, E. (2011). Induction of microspore-derived embryos by anther culture in selected pepper genotypes. Afr. J. Biotechnol. 10, 17116-17121. doi: 10.5897/AJB11.2023