Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey

Abdullah Güller; Mustafa Usta; Gülüstan Korkmaz; Serap Demirel; Zeynelabidin Kurt

doi:10.20289/zfdergi.1081067

EN TR

Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey

Öz

Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms. Material and Methods: In this study, plants collected from Iğdır province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used. Results: In the tests performed, approximately 1.2 kb of DNA fragments specific to phytoplasma were obtained. The 16S rRNA nucleotide sequence (1254 bp in length) (OM663745) revealed that it was showed more than 99.44% nucleotide similarity to other ‘Ca. P. trifolii’ members. The tentative RFLP and phylogenetic analyzes performed proved the ‘Ca. P. trifolii’ the infection from the Clover proliferation group (16SrVI) group and subgroup A in symptomatic pepper plants. Conclusion: The presence of ‘Ca. P. trifolii’ in naturally infected peppers in Iğdır province of Turkey was detected using PCR-RFLP and cladistic analysis.

Anahtar Kelimeler

Clover proliferation group (16SrVI), Nested PCR, Capsicum annuum, RFLP

Destekleyen Kurum

Bulunmamaktadır

Teşekkür

Dergi Editörü ve sekretaryasına teşekkür ederim. Çalışmada emeği geçen tüm arkadaşlarıma teşekkür ederim.

Kaynakça

Alp, Ş., M. Usta, H.M. Sipahioğlu & A. Güller, 2016. First report of "Candidatus Phytoplasma solani" on a new host marigold (Tagetes erecta L.). Turkish Journal of Agriculture & Forestry, 40: 311-318. http: //doi.org/10.3906/tar-1506-58
Arocha, Y., G. Plata, J. Franco, G. Maín, S. Veramendi, F. Lazcano, J.L. Crespo, V. Lino, C. Calderόn, R. Llerena, R. Andrew, O. Antezana, A. Gutiérrez, M. Coca & E. Boa, 2009. First report of a 16SrIII Phytoplasma (X-disease group) affecting bell pepper, strawberry (frutilla), Schinus molle and Arracacia xanthorrhiza in Cochabamba, Bolivia. Plant Pathology, 59 (2): 395-395. http: //doi.org/10.1111/j.1365-3059.2009.02235.x
Bayram, S., U. Zeybekoglu, G. Soylemezoglu, D. Canik, M. Karavin, A. Cakir & F. Ertunc, 2014. Presence of putative insect vectors of grapevine yellows phytoplasmas in Turkey. Phytopathogenic Mollicutes, 4 (1): 22-26. http: //doi.org/10.5958/2249-4677.2014.00578.7
Bertaccini, A. & I.M. Lee, 2018. “Phytoplasmas: an update,”. in: Phytoplasmas: Plant Pathogenic Bacteria-I, in Characterization and Epidemiology of Phytoplasma-Associated Diseases. Phytoplasmas: Plant Pathogenic Bacteria-I, 1-29. http: //doi.org/10.1007/978-981-13-0119-3_1
Bertaccini, A., 2007. Phytoplasmas: diversity, taxonomy and epidemiology. Frontiers in Bioscience, 12: 673-689. http: //doi.org/10.2741/2092
Beşkeçili, M., N. Güneş & M. Gümüş, 2021. Antalya ili Demre ilçesi biber yetiştiriciliğinde Tomato spotted wilt virus (TSWV) ve Cucumber mosaic virus (CMV) etmenlerinin yaygınlığının belirlenmesi, Ege Universitesi Ziraat Fakültesi Dergisi, 58 (3): 399-405. http: //doi.org/10.20289/zfdergi.799432
Bogoutdinov, D.Z., D. Valyunas, M. Navalinskene & M. Samuitene, 2008. About specific identification of Phytoplasmas in solanaceae crops, Sel'skokhozyaistvennaya Biologiya, 1: 77-80.
Chiykowski, L.N., 1965. A yellows-type virus of alsike clover in Alberta. Canadian Journal of Botany, 43: 527-536. http: //doi.org/10.1139/b65-058
Choueiri, E., P. Salar, F. Jreijiri, S. El Zammar, R. Massaad, H. Abdul‐Nour, J.M. Bové, J.L. Jean-Luc Danet & X. Foissac, 2007. Occurrence and distribution of ‘Candidatus Phytoplasma trifolii’ associated with diseases of solanaceous crops in Lebanon. European Journal of Plant Pathology, 118: 411-416. http: //doi.org/10.1007/s10658-007-9142-8
Erdoğan, O., 2009. Bazı pamuk çeşit adaylarının Verticillium solgunluk hastalığı etmeni (Verticillium dahliae Kleb.)'ne karşı duyarlılıklarının belirlenmesi. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 6 (2): 9-16.

Ertunc, F., A. Çakır, G. Soylemezoglu, D.C. Orel, Ş. Topkaya & S. Bayram, 2015. Reactions of some grapevine cultivars to “bois noir” phytoplasma. Phytopathogenic Mollicutes, 5 (1-Supplement): 109-110. http://doi.org/10.5958/2249-4677.2015.00046.8
Faghihi, M.M., S.M. Taghavi, A. Safaei, M. Siampour & S.S. Modarres-Najafabadi, 2016. First report of a Phytoplasma associated with bell pepper big bud disease in Iran. New Disease Reports, 33: 15. http: //doi.org/10.5197/j.2044-0588.2016.033.015
FAO, 2020. Dünya biber üretimi. (Web page: http: //www.fao.org/faostat/) (Date accessed: 23.06.2020).
FAOSTAT, 2017. Crops and livestock products. (Web page: http: //www.fao.org/faostat/en/#data/QC/visualize) (Date accessed: 26.12.2019).
Fialová, R., P. Válová, G. Balakishiyeva, J.L. Danet, D. Šafárová, X. Foissac & M. Navrátil, 2009. Genetic variability of stolbur Phytoplasma in annualcrop and wild plant species in south Moravia. Journal of Plant Pathology, 91: 411-416. http: //doi.org/10.4454/jpp.v91i2.971
Flower, C.E., N. Hayes-Plazolles, J.M. Slavicek & C. Rosa, 2018. First report of ‘Candidatus Phytoplasma trifolii’-related strain of 16SrVI-A Phytoplasma subgroup, associated with Elm yellows disease in American elm (Ulmus americana L.) in Ohio, U.S.A. Plant Disease, 102 (2): 438. http: //doi.org/10.1094/PDIS-08-17-1154-PDN
Girsova, N.V., K.D. Bottner-Parker, D.Z. Bogoutdinov, T.B. Kastalyeva, Y.I. Meshkov, K.A. Mozhaeva & L.I. Ming, 2017. Diverse phytoplasmas associated with leguminous crops in Russia. European Journal of Plant Pathology, 149 (3): 599-610. http: //doi.org/10.1007/s10658-017-1209-6
Güller, A., 2021. Molecular analysis and first-time PCR isolation of ‘Candidatus Phytoplasma Trifolii’ from Maize (Zea mays L.) in Turkey. Fresenius Environmental Bulletin, 30 (10): 11220-11227.
Gundersen, D.E. & I.M. Lee, 1996. Ultrasensitive detection of phytoplasmas by nested-PCR assay using two universal primer pairs. Phytopathologia Mediterranea, 35: 144-151.
Gundersen, D.E., I.M. Lee, D.A. Schaff, N.A. Harrison, C.J. Chang, R.E. Davis & D.T. Kingsbury, 1996. Genomic diversity and differentiation among phytoplasma strains in 16S rRNA groups I (aster yellows and related phytoplasmas) and III (X-disease and related phytoplasmas). International Journal of Systematic Bacteriology, 46: 64-75. http: //doi.org/10.1099/00207713-46-1-64
Harling, R., Y. Arocha, V. Harju, C. Tobing, E. Boa, P. Kelly & R. Reeder, 2009. First report of 16SrII ‘Candidatus Phytoplasma aurantifolia’ infecting chilli and tamarillo in Indonesia. Plant Pathology, 58 (4): 791. http: //doi.org/10.1111/j.1365-3059.2009.02074.x
Hemmati, C., M. Nikooei, & H. Pasalari, 2018. Cota tinctoria and Orosius albicinctus: A new plant host and potential insect vector of ‘Candidatus Phytoplasma trifolii’. Australasian Plant Disease Notes, 13 (1): 13. https: //doi.org/10.1007/s13314-018-0298-1
Hiruki, C. & K.R. Wang, 2004. Clover Proliferation Phytoplasma: ‘Canditatus Phytoplasma trifolii’. International Journal of Systematic and Evolutionary Microbiology, 54: 1349-1353. http: //doi.org/10.1099/ijs.0.02842-0
Hodgetts, J., T. Ball, N. Boonham, R. Mumford & M. Dickinson, 2007. Use of terminal restriction fragment length polymorphism (T-RFLP) for identification of phytoplasmas in plants. Plant Pathology, 56: 357- 365. http: //doi.org/10.1111/j.1365-3059.2006.01561.x
Hogenhout, A.S. & M.S. Music, 2010. Phytoplasma genomics, from sequencing to comparative and functional genomics- What have we learnt? Phytoplasmas Genoms, Plant Hosts and Vectors, 19: 37. http: //doi.org/10.1079/9781845935306.0000
IRPCM, 2004. ‘Candidatus Phytoplasma’, a taxon for the wall‐less, non‐helical prokaryotes that colonize plant phloem and insects. International Journal of Systematic and Evolutionary Microbiology, 54: 1243-1255. http: //doi.org/10.1099/ijs.0.02854-0
Jacobs, K.A., IM. Lee, H.M. Griffiths, F.D.Jr. Miller & K.D. Bottner, 2003. A new member of the clover proliferation phytoplasma group (16SrVI) associated with elm yellows in Illinois. Plant Disease, 87: 241-246. http: //doi.org/10.1094/PDIS.2003.87.3.241
Jomantiene, R., R.E. Davis, J. Maas & E.L. Dally, 1998. Classification of new phytoplasmas associated with diseases of strawberry in Florida, based on analysis of 16S rRNA and ribosomal protein gene operon sequences. International Journal of Systematic Bacteriology, 48: 269-277. http: //doi.org/10.1099/00207713-48-1-269
Julien, H., V. Taffouo, A. Nouck, K. Nyemene, L. Tonfack, T. Meguekam & E. Youmbi, 2017. Effects of Salt Stress on Plant Growth, Nutrient Partitioning, Chlorophyll Content, Leaf Relative Water Content, Accumulation of Osmolytes and Antioxidant Compounds in Pepper (Capsicum annuum L.) Cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45: 481-490. http: //doi.org/10.15835/nbha45210928
Kamran, A., L. Lotos, M.A. Amer, M.A. Al-Saleh, I.M. Alshahwan, M.T. Shakeel, M.H. Ahmad, M. Umar & N.I. Katis, 2018. Characterization of Pepper leafroll chlorosis virus, a New Polerovirus Causing Yellowing Disease of Bell Pepper in Saudi Arabia. Plant Disease, 102: 318-326. http: //doi.org/ 10.1094/PDIS-03-17-0418-RE
Khan, M.S. & S.K. Raj, 2006. First report of molecular detection of an Aster yellows Phytoplasma (Candidatus Phytoplasma asteris) isolate infecting chilli (Capsicum annuum) in India. Plant Pathology, 55: 822. http: //doi.org/10.1111/j.1365-3059.2006.01482.x
Lam, N., R. Creamer, J.Rascon & R. Belfon, 2009. Characterization of a new curtovirus Pepper yellow dwarf virus from chile pepper and distribution in weed hosts in New Mexico. Archives of Virology, 154 (3): 429-436. http: //doi.org/10.1007/s00705-009-0320-1
Lee I.M., D.E. Gundersen-Rindal & R.E.Davis & I.M. Bartoszyk, 1998. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic and Evolutionary Microbiology, 48, 1153-1169. https: //doi.org/10.1099/00207713-48-4-1153
Lee, I.M. & R.E. Davis, 1992. “Mycoplasmas Which Infect Plant and Insects, 379-390”. In: Mycoplasmas: Molecular Biology and Pathogenesis (Eds. J. Maniloff, R.N. McElhansey,L.R. Finch & J.B. Baseman). Washington, DC: American Society of Microbiology, USA.
Lee, I.M., R.E. Davis & D.E. Gundersen-Rindal, 2000. Phytoplasmas: phytopathogenic mollicutes. Annual Review of Microbiology, 56: 1593-1597. http: //doi.org/10.1146/annurev.micro.54.1.221
Lee, I.M., R.W. Hammond, R.E. Davis & D.E. Gundersen, 1993. Universal amplifcation and analysis of pathogen 16S rDNA for classifcation and identifcation of mycoplasma like organisms. Phytopathology, 83: 834-842.
Navratil, M., P. Valova, R. Fialova, P. Lauterer, D. Safarova & M. Stary, 2009. The incidence of “stolbur” disease and associated yield losses in vegetable crops in South Moravia (Czech Republic). Crop Protection, 28: 898-904. http: //doi.org/10.1016/j.cropro.2009.05.008
Oksal, H.D., 2020. Natural Phytoplasma infections on fruit, vegetable and weed plants at the same agroecosystem and their molecular properties. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48 (2): 615-625. http: //doi.org/10.15835/nbha48211940
Oksal, H.D., F. Kaya Apak, E. Oksal, T. Tursun & H.M. Sipahioglu, 2017. Detection and Molecular Characterization of two ‘Candidatus Phytoplasma Trifolii’ Isolates Infecting Peppers at the Same Ecological Niche. International Journal of Agriculture & Biology, 19 (6): 1372-1378. http: //doi.org/10.17957/IJAB/15.0420
Özdağ, Y. & G. Sertkaya, 2017. Investigation on viruses causing yellowing disease in pepper in Hatay-Turkey. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 22: 16-22.
Rao, G.P. & M. Kumar, 2017. World status of Phytoplasma diseases associated with eggplant. Crop Protection, 96: 22-29. http: //doi.org/10.1016/j.cropro.2017.01.005
Saitou, N. & M. Nei, 1987. The Neighbor-Joining method: A new method for reconstructing phylogenetic tree. Molecular Biology and Evolution, 4: 406-425. http: //doi.org/10.1093/oxfordjournals.molbev.a040454
Salas-Muñoz, S., J.A. Mauricio-Castillo, C.H. Dietrich, R. Creamer & L.R. Reveles-Torres, 2018. First report of the leafhoppers Ceratagallia nitidula and Empoasca abrupta (Hemiptera: Cicadellidae) as vectors of 'Candidatus Phytoplasma trifolii'. Plant Disease, 102 (12): 2636-2637. http: //doi.org/10.1094/PDIS-06-18-0975-PDN
Santos-Cervantes, M.E., J.A. Chávez-Medina, J. Méndez-Lozano & N.E. Leyva-López, 2008. Detection and Molecular Characterization of Two Little Leaf Phytoplasma Strains Associated with Pepper and Tomato Diseases in Guanajuato and Sinaloa, Mexico. Plant Disease, 92 (7): 1007-1011. http: //doi.org/10.1094/PDIS-92-7-1007
Sertkaya, G., M. Martini & R. Osler, 2008. First report of ‘Candidatus Phytoplasma mali’ in Turkey. Journal of Plant Pathology, 90: 143-149.
Sertkaya, G., M. Martini, R. Musetti & R. Osler, 2007. Detection and molecular characterization of Phytoplasmas infecting sesame and solanaceous crops in Turkey. Bulletin of Insectology, 60 (2): 141-142.
Şevik, M.A., 2011. Occurrence of pepper mild mottle virus in greenhouse-grown pepper (Capsicum annuum L.) in the West Mediterranean region of Turkey. African Journal of Biotechnology, 10 (25): 4976-4979.
TUIK, 2020. Bitkisel üretim istatistikleri. (Web page: https: //biruni.tuik.gov.tr/medas/?locale=tr) (Date accessed: 13.02.2022).
Usta, M. & A. Güller, 2020. Detection and molecular characterization of ‘Candidatus Phytoplasma Trifolii’, a member of the Clover proliferation grup, infecting tomato plants from Iğdır Province in Turkey. Turkish Journal of Agriculture - Food Science and Technology, 8 (12): 2533-2540. http: //doi.org/10.24925/turjaf.v8i12.2533-2540.3592
Usta, M., A. Güller & H.M. Sipahioğlu, 2017. Detection and characterization of two Phytoplasma lineages on cucumber (Cucumis sativus L.) with same symptomatology based on Virtual RFLP and Nucleotide Sequence Analysis of 16S rDNA. Yuzuncu Yil University Journal of Agricultural Sciences, 27 (3): 299-308. https://doi.org/10.29133/yyutbd.288346
Usta, M., A. Güller & H.M. Sipahioğlu, 2021. First report of ‘Candidatus Phytoplasma trifolii’ associated with leaf reddening and upright growth in pears (Pyrus communis L.). Plant Protection Science, 57 (3): 188-195. http: //doi.org/10.17221/163/2020-PPS
Usta, M., A. Güller & H.M. Sipahioğlu, 2022. Detection, in silico analysis and molecular diversity of phytoplasmas from solanaceous crops in Turkey. Plant Protection Science, 58: 31-39. http: //doi.org/10.17221/115/2021-PPS Weintraub, P.G & L. Beanland, 2006. Insect vector of Phytoplasmas. Annual Review of Entomology, 51: 91-111. http: //doi.org/10.1146/annurev.ento.51.110104.151039
Yılmaz, S., B.K. Çağlar & K. Djelouah, 2019. Molecular characterization of Phytoplasma diseases of pepper in Turkey. Journal of Phytopathology, 167: 479-483. http: //doi.org/10.1111/jph.12820
Zhao, Y., W. Wei, I.M. Lee, J. Shao, X. Suo & R.E. Davis, 2009. Construction of an interactive online Phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease Phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology, 59: 2582-2593. http: //doi.org/10.1099/ijs.0.010249-0
Zheng-Nan, L., L. Zhang, J.G. Song & Y.F. Wu, 2013. Molecular detection and identification of Phytoplasma associated with pepper witches‟ broom in China. Phytoparasitica, 41: 429-434. http: //doi.org/10.1007/s12600-013-0304-2

Ayrıntılar

Birincil Dil

İngilizce

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Abdullah Güller ^*
0000-0003-3887-4208
Türkiye

Mustafa Usta
0000-0002-3940-2774
Türkiye

Gülüstan Korkmaz
0000-0002-9032-5823
Türkiye

Serap Demirel
0000-0002-1877-0797
Türkiye

Zeynelabidin Kurt
0000-0003-1164-9326
Türkiye

Yayımlanma Tarihi

30 Eylül 2022

Gönderilme Tarihi

1 Mart 2022

Kabul Tarihi

17 Mayıs 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 59 Sayı: 3

DOI

https://doi.org/10.20289/zfdergi.1081067

IZ

https://izlik.org/JA89NR48EY

APA

Güller, A., Usta, M., Korkmaz, G., Demirel, S., & Kurt, Z. (2022). Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University, 59(3), 429-437. https://doi.org/10.20289/zfdergi.1081067

AMA

1.Güller A, Usta M, Korkmaz G, Demirel S, Kurt Z. Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University. 2022;59(3):429-437. doi:10.20289/zfdergi.1081067

Chicago

Güller, Abdullah, Mustafa Usta, Gülüstan Korkmaz, Serap Demirel, ve Zeynelabidin Kurt. 2022. “Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey”. Journal of Agriculture Faculty of Ege University 59 (3): 429-37. https://doi.org/10.20289/zfdergi.1081067.

EndNote

Güller A, Usta M, Korkmaz G, Demirel S, Kurt Z (01 Eylül 2022) Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University 59 3 429–437.

IEEE

[1]A. Güller, M. Usta, G. Korkmaz, S. Demirel, ve Z. Kurt, “Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey”, Journal of Agriculture Faculty of Ege University, c. 59, sy 3, ss. 429–437, Eyl. 2022, doi: 10.20289/zfdergi.1081067.

ISNAD

Güller, Abdullah - Usta, Mustafa - Korkmaz, Gülüstan - Demirel, Serap - Kurt, Zeynelabidin. “Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey”. Journal of Agriculture Faculty of Ege University 59/3 (01 Eylül 2022): 429-437. https://doi.org/10.20289/zfdergi.1081067.

JAMA

1.Güller A, Usta M, Korkmaz G, Demirel S, Kurt Z. Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University. 2022;59:429–437.

MLA

Güller, Abdullah, vd. “Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey”. Journal of Agriculture Faculty of Ege University, c. 59, sy 3, Eylül 2022, ss. 429-37, doi:10.20289/zfdergi.1081067.

Vancouver

1.Abdullah Güller, Mustafa Usta, Gülüstan Korkmaz, Serap Demirel, Zeynelabidin Kurt. Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University. 01 Eylül 2022;59(3):429-37. doi:10.20289/zfdergi.1081067