Asmada (Vitis vinifera cv. Sultani Çekirdeksiz) Bax İnhibitor-1 Geninin İzolasyonu ve Moleküler Karakterizasyonu

Birsen Çakır

doi:10.20289/zfdergi.69696

EN TR

Asmada (Vitis vinifera cv. Sultani Çekirdeksiz) Bax İnhibitor-1 Geninin İzolasyonu ve Moleküler Karakterizasyonu

Öz

P rogramlanmış hücre ölümü (PCD) canlılarda, gelişmede ve çevresel stres faktörlerinin etkisi ile zarar görmüş hücrelerin elimine edilmesinde önemli bir fizyolojik olaydır. Bax inhibitor-1 (BI-1) proteininin hücre ölümünü engellediği ve ökaryotlarda yüksek oranda korunduğu gösterilmiştir. Bu çalışmada, Vitis vinifera cv. Sultani Çekirdeksiz çeşidinin üzüm tanelerinden gen izole edilmiş ve VvBI-1 olarak adlandırılarak gen bankasına kayıt altına alınmıştır. Olası VvBI-1 geninin protein kodlayan kısmı 747 bç uzunluğundadır ve protein büyüklüğü de 248 aa olarak bulunmuştur. VvBI-1 protein dizisinin 27,290 kDa büyüklüğünde olduğu ve olası izoelektrik noktasının da 8,62 olduğu öngörülmüştür. Farklı gelişme dönemlerinde hasat edilmiş üzüm tanelerinden semi quantitative RT-PCR analizi ile VvBI-1 geninin ifade profili çıkarılmış ve VvBI-1 ifadesinin yüksek oranda ve tüm gelişme dönemlerinde var olduğu gözlemlenmiştir

Anahtar Kelimeler

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Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

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Yazarlar

Birsen Çakır Bu kişi benim

Yayımlanma Tarihi

1 Eylül 2012

Gönderilme Tarihi

25 Kasım 2015

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2012 Cilt: 49 Sayı: 3

DOI

https://doi.org/10.20289/zfdergi.69696

IZ

https://izlik.org/JA96ZN33XM

APA

Çakır, B. (2012). Asmada (Vitis vinifera cv. Sultani Çekirdeksiz) Bax İnhibitor-1 Geninin İzolasyonu ve Moleküler Karakterizasyonu. Journal of Agriculture Faculty of Ege University, 49(3), 249-254. https://doi.org/10.20289/zfdergi.69696