Isolation and molecular characterization of Bax Inhibitor-1 Gene from Grapevine (Vitis vinifera cv. Sultanina)

Yıl 2012, , 249 - 254, 01.09.2012

Birsen Çakır

https://doi.org/10.20289/zfdergi.69696

Cited By: 1

Öz

Anahtar Kelimeler

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Asmada (Vitis vinifera cv. Sultani Çekirdeksiz) Bax İnhibitor-1 Geninin İzolasyonu ve Moleküler Karakterizasyonu

Öz

P rogramlanmış hücre ölümü (PCD) canlılarda, gelişmede ve çevresel stres faktörlerinin etkisi ile zarar görmüş hücrelerin elimine edilmesinde önemli bir fizyolojik olaydır. Bax inhibitor-1 (BI-1) proteininin hücre ölümünü engellediği ve ökaryotlarda yüksek oranda korunduğu gösterilmiştir. Bu çalışmada, Vitis vinifera cv. Sultani Çekirdeksiz çeşidinin üzüm tanelerinden gen izole edilmiş ve VvBI-1 olarak adlandırılarak gen bankasına kayıt altına alınmıştır. Olası VvBI-1 geninin protein kodlayan kısmı 747 bç uzunluğundadır ve protein büyüklüğü de 248 aa olarak bulunmuştur. VvBI-1 protein dizisinin 27,290 kDa büyüklüğünde olduğu ve olası izoelektrik noktasının da 8,62 olduğu öngörülmüştür. Farklı gelişme dönemlerinde hasat edilmiş üzüm tanelerinden semi quantitative RT-PCR analizi ile VvBI-1 geninin ifade profili çıkarılmış ve VvBI-1 ifadesinin yüksek oranda ve tüm gelişme dönemlerinde var olduğu gözlemlenmiştir

Anahtar Kelimeler

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Kaynakça

• Bolduc, N., and L. F. Brisson, 2002. Antisense down regulation of NtBI-1 in tobacco BY-2 cells induces accelerated cell death upon carbon starvation. FEBS Letters 532(1‚Äì2): 111-114.
• Bolduc, N., M. Ouellet, F. Pitre, and L. Brisson, 2003. Molecular characterization of two plant BI-1 homologues which suppress Bax-induced apoptosis in human 293 cells. Planta 216(3): 377- 386.
• Bras, M., B. Queenan and S.A. Susin, 2005. Programmed Cell Death via Mitochondria: Different Modes of Dying. Biochemistry (Mosc) 70(2): 231-239.
• Datta, R. S., C. Meacham, B. Samad, C. Neyer and K. Sjalander,
• Chae, H.-J., N. Ke, H.-R. Kim, S. Chen, A. Godzig, M. Dickman and J.C. Reed, 2003. Evolutionarily conserved cytoprotection provided by Bax Inhibitor-1 homologs from animals, plants, and yeast. Gene 323(0): 101-113.
• Coupe, S. A., L. M. Watson, D.J. Ryan, T.T. Pinkley and J.R. Eason, 2004. Molecular analysis of programmed cell death during senescence in Arabidopsis thaliana and Brassica oleracea: cloning broccoli LSD1, Bax inhibitor and serine palmitoyltransferase homologues. Journal of Experimental Botany 55(394): 59-68.
• Danon, A., V. Delorme, N. Mailhac and P. Gallois, 2000. Plant programmed cell death: A common way to die. Plant Physiology and Biochemistry 38(9): 647-655. 2009. Berkeley PHOG: PhyloFacts orthology group prediction web server. Nucleic Acids Research 37(suppl 2): W84-W89.
• Davies, C. and S.P. Robinson, 1996. Sugar Accumulation in Grape Berries (Cloning of Two Putative Vacuolar Invertase cDNAs and Their Expression in Grapevine Tissues). Plant Physiology 111(1): 275-283.
• Eichmann, R., H. Schultheiss, K.H. Kogel and R. Hückelhoven, 2004. The Barley Apoptosis Suppressor Homologue Bax Inhibitor-1 Compromises Nonhost Penetration Resistance of Barley to the Inappropriate Pathogen Blumeria graminis f. sp. tritici. Molecular Plant-Microbe Interactions 17(5): 484-490.
• Ellis, H. M. and H. R. Horvitz, 1986. Genetic control of programmed cell death in the nematode C. elegans. Cell 44(6): 817-829.
• Hengartner, M. O., R. Ellis and R. Horvitz, 1992. Caenorhabditis elegans gene ced-9 protects cells from programmed cell death. Nature 356(6369): 494-499.
• Henke, N., D. A. Lisak, L. Schneider, J. Habicht, M. Pergande and A. Methner, 2011. The ancient cell death suppressor BAX inhibitor-1. Cell Calcium 50(3): 251-260.
• Hückelhoven, R., 2004. BAX Inhibitor-1, an ancient cell death suppressor in animals and plants with prokaryotic relatives. Apoptosis 9(3): 299-307.
• Ihara-Ohori, Y., M. Nagano, S. Muto, H. Uchimiya and M. Kawai- Yamada, 2007. Cell Death Suppressor Arabidopsis Bax Inhibitor-1 Is Associated with Calmodulin Binding and Ion Homeostasis. Plant Physiology 143(2): 650-660.
• Isbat, M., N. Zeba, S.R. Kim and C.B. Hong, 2009. A BAX inhibitor-1 gene in Capsicum annuum is induced under various abiotic stresses and endows multi-tolerance in transgenic tobacco. Journal of Plant Physiology 166(15): 1685-1693.
• Kacprzyk, J., C. T. Daly and P.F. McCabe, 2011. The Botanical Dance of Death: Programmed Cell Death in Plants. Advances in Botanical Research. K. Jean-Claude and D. Michel, Academic Press. Volume 60: 69-261.
• Kawai, M., L. Pan, J.C. Reed and H. Uchimia, 1999. Evolutionally conserved plant homologue of the Bax Inhibitor-1 (BI-1) gene capable of suppressing Bax-induced cell death in yeast. FEBS Letters 464(3): 143-147.
• Kawai, M., P. K. Samarajeewa, R.A. Barrero, M. Nishiguchi and H. Uchimiya, 1998. Cellular dissection of the degradation pattern of cortical cell death during aerenchyma formation of rice roots. Planta 204(3): 277-287.
• Kawai-Yamada, M., Y. Ohori and H. Uchimiya, 2004. Dissection of Arabidopsis Bax Inhibitor-1 Suppressing Bax "Hydrogen Peroxide and Salicylic Acid" Induced Cell Death. The Plant Cell Online 16(1): 21-32.
• Lacomme, C. and S. Santa Cruz, 1999. Bax-induced cell death in tobacco is similar to the hypersensitive response. Proceedings of the National Academy of Sciences 96(14): 7956-7961.
• Lockshin, R. A. and Z. Zakeri, 2004. Apoptosis, autophagy, and more. The International Journal of Biochemistry & Cell Biology 36(12): 2405-2419.
• Matsumura, H., S. Nirasawa, A. Kiba, N. Urasaki, H. Saitoh, M. Ito, M. Kawai-Yamada, H. Uchimiya and R. Terauchi, 2003. Overexpression of Bax inhibitor suppresses the fungal elicitor- induced cell death in rice (Oryza sativa L.) cells. The Plant Journal 33(3): 425-434.
• Nakashima, T., T. Sekiguchi, A. Kuraoka, K. Fukushima, Y. Shibata, S. Komiyama and T. Nishimoto, 1993. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Molecular and Cellular Biology 13(10): 6367-6374.
• Orzaez, D. and A. Granell, 1997. The plant homologue of the defender against apoptotic death gene is down-regulated during senescence of flower petals. FEBS Letters 404(2‚Äì3): 275-278.
• Pecorino, L., 2008. Apoptosis. Molecular Biology of Cancer - Mechanisms Targets and Therapeutics. London, Oxford: 137-160.
• Pennell, R. and C. Lamb, 1997. Programmed Cell Death in Plants. The Plant Cell 9: 1157-1168.
• Reape, T. J., E. M. Molony and P.F. McCabe, 2008. Programmed cell death in plants: distinguishing between different modes. Journal of Experimental Botany 59(3): 435-444.
• Samarajeewa, P. K., R. A. Barrero, C. Umeda-Hara, M. Kawai and H. Uchimiya, 1999. Cortical cell death, cell proliferation, macromolecular movements and Tip1; expression pattern in roots of rice (Oryza sativa; L.) under NaCl stress. Planta 207(3): 354-361.
• Sanchez, P., M. De Torres Zabala and M. Grant, 2000. AtBI-1, a plant homologue of Bax Inhibitor-1, suppresses Bax-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge. The Plant Journal 21(4): 393-399.
• Watanabe, N. and E. Lam, 2006. Arabidopsis Bax inhibitor-1 functions as an attenuator of biotic and abiotic types of cell death. The Plant Journal 45(6): 884-894.
• Watanabe, N. and E. Lam, 2008. Arabidopsis bax inhibitor-1: A rheostat for ER stress-induced programmed cell death. Plant Signaling & Behavior 3(8): 564-566.
• Xu, Q. and J. C. Reed, 1998. Bax Inhibitor-1, a Mammalian Apoptosis Suppressor Identified by Functional Screening in Yeast. Molecular Cell 1(3): 337-346.