Potansiyel Ekşi Hamur Starter Kültürü Weissella cibaria N9'un Dondurularak Kurutulması için Koruyucu Ajanların Optimizasyonu

Year 2021, Volume: 19 Issue: 2, 137 - 149, 01.08.2021

Latife Betül Gül Osman Gül Enes Dertli Ahmet Hilmi Çon

https://doi.org/10.24323/akademik-gida.977267

Abstract

Bu çalışmada ekşi hamurdan izole edilmiş ve starter kültür olarak kullanılabileceği belirlenmiş Weissella cibaria N9 suşunun liyofilizasyonu için optimum kriyoprotektan formülasyonunun belirlenmesi, liyofilize kültürün karakterizasyonu ve depolama stabilitelerinin belirlenmesi amaçlanmıştır. Liyofilizasyon sonrası yüksek canlılık sağlamak için kullanılacak yağsız süt tozu (YST), laktoz ve sükroz’dan oluşan optimum formülasyon Box Behnken tasarımı kullanılarak belirlenmiştir. Optimum kriyoprotektan formülasyonu yüksek canlılık (>%99) için %5.65 YST, %20 laktoz ve %9.38 sükroz şeklinde tanımlanmıştır. Optimum kriyoprotektan formülasyonu kullanılarak elde edilen liyofilize kültürün nem içeriği, aw, camsı geçiş, partikül yüzey özellikleri ve kristal yapı bakımından kabul edilebilir fizikokimyasal özelliklere sahip olduğu gözlenmiştir. 3.37x10-3 1/gün inaktivasyon katsayısı ile en yüksek canlılık (9.11 log kob/g) 4C’de depolama sonunda elde edilmiştir. Sıcaklığa bağlı hızlandırılmış raf ömrü testi sonucu en hızlı canlılık kaybı 70C’de gözlenmiş olup kriyoprotektan kullanımı termal ölüm oranını azaltmıştır. Kriyoprotektan kullanılarak üretilen kültürün oda sıcaklığında 18 ay saklanabileceği belirlenmiştir. Sonuç olarak, optimum kriyoprotektan formülasyonu W. cibaria N9’un liyofilizasyonu ve depolama sırasında hücre canlılığını korumada etkili olduğu, toz materyaller için gerekli özellikleri taşıdığı ve uzun dönem muhafaza için canlılığın yeterli hassasiyette tahmin edilmesinde sıcaklığa bağlı hızlandırılmış raf ömrü testinin faydalı bir teknik olduğu tespit edilmiştir.

Keywords

Kriyoprotektan, Liyofilizasyon, Weissella cibaria, Depolama stabilitesi, Yanıt yüzey metodu, Optimizasyon

Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Project Number

TOVAG 117O159

Thanks

Latife Betül GÜL’ün doktora tezinin bir kısmı olan bu çalışmayı TOVAG 117O159 proje numarası ile maddi olarak destekleyen Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)’a teşekkür ederiz.

Optimization of Protective Agents for Freeze-Drying of Weisella cibaria N9 as a Potential Starter Culture

Abstract

In this study, it was aimed to determine the optimum cryoprotectant formulation for the lyophilization of Weissella cibaria N9 strain isolated from sourdough as a potential starter culture, the characterization of lyophilized culture and its storage stability. The optimum formulation of skim milk, lactose and sucrose as protective agents was determined by the Box-Behnken experimental design based on viability after lyophilization. The optimal cryoprotectant formulation was identified as 5.65% skim milk, 20% lactose and 20% sucrose for maximum cell survival (>99%). Lyophilized culture obtained by the optimum cryoprotectant formulation had acceptable physicochemical properties in terms of moisture and aw, glass transition, particle surface properties and crystal structure. The highest viability was observed at 4C (9.11 log cfu/g) with an inactivation rate of 3.37x10-3 day-1. The fastest cell reduction was observed at 70C as the result of an accelerated shelf life storage test, and protective agent effectively decreased thermal death. The culture produced by using cryoprotectant could be stored at room temperature for 18 months. Consequently, this protective agent formulation was an effective in protecting W. cibaria N9 viability during lyophilization and storage while lyophilized culture had ideal properties for powder materials, and accelerated shelf life storage test was a useful technique with certain predictability.

Keywords

Cryoprotectants, Lyophilization, Weissella cibaria, Storage stability, Response surface method, Optimization

Project Number

TOVAG 117O159

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