Effects of Thidiazuron and Activated Charcoal on in vitro Shoot Proliferation and Rooting of Myrtle (Myrtus communis L.)

Abstract

In this study, a micropropagation approach was developed for a commercially important myrtle clone ‘Aşı Mersin’ in Turkey. The effects of different concentrations of thidiazuron (TDZ), 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA) on shoot formation and the effect of activated charcoal on rooting were studied. The most of the shoots were obtained on Murashige and Skoog (MS) medium containing 0.3 mg L-1 TDZ + 0.1 mg L-1 NAA in the 1st subculture (3.8 shoot), and from MS with 0.3 mg L-1 TDZ + 0.01 mg L-1 NAA in the 2nd subculture (4.0 shoot). The explants were rooted in ½MS containing indole-3-butyric acid (IBA), NAA and activated charcoal (AC). IBA applications induced more rooting than NAA. The medium supplemented with 1.0 mg L-1 IBA + 2.0 g L-1 AC resulted in the highest rooting ratio (80%). Addition of AC into the medium resulted in slight increase in the rooting ratio, significant increase in shoot length, and reduced darkening in the rooting area. Acclimatization was successful for 86% of the rooted plants

Keywords

• Myrtle
• Proliferation
• Activated charcoal
• Thidiazuron
• Acclimatization

Effects of Thidiazuron and Activated Charcoal on in vitro Shoot Proliferation and Rooting of Myrtle (Myrtus communis L.)

Öz

Çalışmada, Mersin bitkisinin ticari öneme sahip “Aşı Mersin” klonu için bir mikro çoğaltım yöntemi geliştirilmiştir. Bu amaçla farklı konsantrasyonlarda thidiazuron (TDZ), 6-benzil amino pürin (BAP) ve naftalin asetik asit’in (NAA) sürgün proliferasyonu ve aktif karbonun (AK) köklenme üzerine etkileri belirlenmiştir. En yüksek sürgün sayıları 1. alt kültürde 3.8 adet ile 0.3 mg L-1 TDZ + 0.1 mg L-1 NAA içeren Murashige ve Skoog (MS) ortamından elde edilirken, 2. alt kültürde 4.0 adet ile 0.3 mg L-1 TDZ + 0.01 mg L-1 NAA içeren MS ortamından elde edilmiştir. İn vitro sürgünler indole-3-butyric acid (IBA), NAA ve aktif kömür içeren ½MS ortamında köklendirilmiştir. IBA uygulamalarının NAA uygulamalarına göre köklenmeyi daha fazla uyardığı belirlenmiştir. İçinde 1.0 mg L-1 IBA + 2.0 g L-1 AC bulunan ½MS ortamı en yüksek köklenme oranını (% 80) vermiştir. Ortama AC eklenmesi köklenme oranını nispeten ve sürgün uzunluğunu önemli derecede arttırmış ve kök bölgesindeki kararmayı ise azaltmıştır. Köklenmiş bitkilerin % 86’sı dış koşullara başarılı bir şekilde alıştırılmıştır

Anahtar Kelimeler

• Myrtus communis
• Acclimatization
• Activated charcoal
• Thidiazuron

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