The Antibacterial Effects of Çiriş (Asphodelus aestivus Brot.) on Some Foodborne Pathogenic Bacteria

Abstract

This study aimed to determine the antibacterial effects of Çiriş (Asphodelus aestivus Brot.) on eight important food-borne pathogenic bacteria by the disc diffusion method. The highest inhibition effect was determined on Escherichia coli with a 15.63 mm zone diameter, followed by, Staphylococcus aureus (14.78 mm), Listeria monocytogenes (10.72 mm), Shigella flexneri (10.06 mm), Enterobacter aerogenes (9.22 mm) and Enterococcus feacalis (8.15 mm) (P<0.05). On the other hand, Çiriş showed the lowest inhibition effect on Salmonella Typhi and Salmonella Typhimirium, with 7.28 and 6.42 mm zone diameters, respectively. Comparing the data obtained to the effects of the standard antibiotics on the same pathogens, it was found that Escherichia coli were susceptible while Staphylococcus aureus and Shigella flexneri were moderately susceptible to the ethanol extract of Çiriş. On the other hand, it was determined that Salmonella Typhi, Listeria monocytogenes, Enterococcus feacalis, Salmonella Typhimirium, and Enterobacter aerogenes exhibited resistance.

Keywords

• Asphodelus aestivus Brot.,Pathogens,Antibacterial effect

Çirişin (Asphodelus aestivus Brot.) Bazı Gıda Kaynaklı Patojen Bakteriler Üzerindeki Antibakteriyel Etkisi

Abstract

Bu araştırmada çirişin (Asphodelus aestivus Brot.) sekiz önemli gıda kaynaklı patojen bakteri üzerindeki antibakteriyel etkisinin disk difüzyon metodu ile belirlenmesi amaçlanmıştır. Araştırma sonucunda en fazla inhibisyon etkinin; 15.63 mm zon çapı Escherichia coli üzerinde olduğu, bunu sırasıyla Staphylococcus aureus (14.78 mm), Listeria monocytogenes (10.72 mm), Shigella flexneri (10.06 mm), Enterobacter aerogenes (9.22 mm) ve Enterococcus feacalis (8.15 mm) takip ettiği belirlenmiştir (P<0.05). Buna karşın en düşük inhibisyon etkinin ise; sırasıyla 7.28 ve 6.42 mm zon çaplarıyla Salmonella Typhi ve Salmonella Typhimirium üzerinde olduğu gözlemlenmiştir. Elde edilen veriler aynı patojenler üzerinde standart antibiyotiklerin etkileri ile kıyaslandığında çirişin etanol ekstraktına karşı; Escherichia coli’ nin duyarlı, Staphylococcus aureus ve Shigella flexneri’nin orta duyarlı olduğu belirlenmiştir. Buna karşın; Salmonella Typhi, Listeria monocytogenes, Enterococcus feacalis, Salmonella Typhimirium ve Enterobacter aerogenes’in ise, dirençli olduğu tespit edilmiştir.

Keywords

• Asphodelus aestivus Brot.,Patojen,Antibakteriyel etki

References

1. Acibuda, V. & Bostan-Budak, D. (2018) The place and importance of medicinal and aromatic plants in the World and Turkey. Cukurova Journal of Agricultural Food Sciences, 33(1), 37-44.
2. Akarca, G. (2019). Composition and antibacterial effect on food borne pathogens of Hibiscus surrattensis L. calyces essential oil. Industrial Crops & Products, 137, 285-289. https://doi.org/10.1016/j.indcrop.2019.05.043
3. Akarca, G., Tomar, O., Guney, I., Erdur, S. & Gok, V. (2019) Determination of sensivity of some food pathogens to spice extract. Journal of Food Science and Technology, 56(12), 5253-5261. https://doi.org/10.1007/s13197-019-03994-1
4. Akcicek, E. & Vural, M. (2007). The endemic and rare non-endemic plants of Kumalar Mountain (Afyonkarahisar). J. BAUN Inst. Sci. Technol, 9(2),78-86.
5. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol, 36, 493-496.
6. Bayrak, B. B. (2013). Antioxidant activities of Çiriş (Eremurus Spectabilis Bieb.) and some sulfur compounds, Ph.D. Thesis, İstanbul University, Graduate school of natural and applied sciences, İstanbul, Turkey.
7. Baytop, T. (1999). Treatment with plants in Turkey, Nobel Medical Bookstores, Istanbul, Turkey.
8. CLSI. (2015). Clinical and Laboratory Standards Institute, Zone diameter and minimal inhibitory concentration (MIC) Standards.

9. Eucast. (2018). European Committee on Antimicrobial Susceptibility Testing, http://www.eucast.org/fileadmin/src /media/PDFs/EUCAST_files/Breakpoint_tables/v_8.0_Breakpoint_Tables.pdf
10. Fatal, T., Yilmaz, F. F., Birincioglu, S. S., Hosgor-Limoncu, M. & Kivrak, B. (2016). Fatty acid composition and antimicrobial activity of Asphodelus aestivus seeds. Human & Veterinary Medicine, International Journal of the Bioflux Society, 8(2), 103-107.
11. Karatepe, P. & Patir, B. (2012). The effect of eugenol and thymol on chemical, microbiological and sensory quality of pasteurized butter. Firat University Medical Journal of Health Sciences, 26, 35-46.
12. Li, C., Shi, J. G., Zhang, Y, P. & Zhang, C. Z. (2000). Constituents of Eremurus chinensis, Journal of Natural Products, 63, 653-656.
13. Oskay, M., Tamer, A. U., Ay, G., Sari, D. & Akta, K. (2005) Antimicrobial activity of the leaves of Lippia triphylla (L'Her) O. Kuntze (Verbenaceae) against on bacteria and yeasts. Journal of Biological Sciences, 5, 620-622.
14. Oskay, M., Aktas, K., Sari, D. & Azeri, C. A. (2007). Comparative study of antimicrobial activity using well and disk diffusion method on Asphodelus aestivus (Liliaceae). Ekoloji, 16, 62-65.
15. Peksel, A., Altas-Kiymaz, N. & Imamoglu, S. (2012). Evaluation of antioxidant and antifungal potential of Asphodelus aestivus Brot. growing in Turkey. Journal of Medicinal Plants Research, 62, 253-265.
16. Silva, F. & Domingues, F. C. (2017). Antimicrobial activity of coriander oil and its effectiveness as food preservative. Crit. Rev. Food Sci. Nutr, 57, 35-47.
17. Ugurlu, I., Baslar, S., Yorek, N. & Dogan, Y. (2009). The investigation and quantitative ethnobotanical evaluation of medicinal plants used around Izmir province, Turkey. J. Med. Plant Res, 3(5), 345-367.
18. Zhang, Y. P., Zhang, C. Z., Tao, B. Q. & Li, C. (2000). Chemical constituents from Eremurus chinensis Fedtsch. China Journal of Chinese Materia Medica, 25, 355-356.