Year 2021, Volume 46 , Issue 3, Pages 692 - 706 2021-05-15

DETERMINATION OF STAPHYLOCOCCUS AUREUS PREVALENCE AND ANTIBIOTIC RESISTANCE PROFILES, ANTIBIOTIC RESISTANCE AND ENTEROTOXIN GENES IN POULTRY MEATS
KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ

Çilem KISA [1] , Yasin TUNCER [2]


In this study, it was aimed to investigate the prevalence of Staphylococcus aureus in poultry meats (32 chickens, 9 turkeys, 9 quails) sold in Isparta and Antalya provinces, and to determine the presence of enterotoxin genes as well as antibiotic resistance profiles and resistance genes. Baird Parker agar medium was used to isolate presumptive S. aureus from poultry meats. The species-level identification of isolates was done by polymerase chain reaction (PCR) using primer pair specific to the thermostable nuclease gene (nuc) in S. aureus. As a result of the PCR analysis, amplicons of 458 bp specific to the nuc gene were obtained in 16 of 130 presumptive S. aureus isolates. The frequency of S. aureus in poultry meat samples was found to be 20% (10/50). Coagulase test showed that all the S. aureus isolates were coagulase positive. Disk diffusion test showed that all of the isolates (100 %) were susceptible to chloramphenicol and teicoplanin and resistant to penicillin G. It was determined that 81.25 % (13/16) of the isolates were methicillin resistant S. aureus (MRSA). The most common antibiotic resistance gene in S. aureus strains was found to be blaZ (62.5 %, 10/16) as a result of PCR. The presence of sea, seb, sec, sed and see genes was not detected in any of the S. aureus strains.
Bu çalışmada, Isparta ve Antalya illerinde perakende satışı yapılan kanatlı etlerinde (32 piliç, 9 hindi, 9 bıldırcın) Staphylococcus aureus yaygınlığının araştırılması, antibiyotik direnç profilleri ve direnç genlerinin yanı sıra enterotoksin genleri varlığının belirlenmesi amaçlanmıştır. Kanatlı etlerinden muhtemel S. aureus izolasyonunda Baird Parker agar besiyeri kullanılmıştır. İzolatların tür düzeyinde tanısı S. aureus’da termostabil nükleaz genine (nuc) özgü primer çifti kullanılarak polimeraz zincir reaksiyonu (PZR) ile yapılmıştır. 130 muhtemel S. aureus izolatının 16’sında nuc genine özgü 458 bç büyüklüğünde amplikonlar elde edilmiştir. Kanatlı eti örneklerinde S. aureus bulunma sıklığı % 20 (10/50) bulunmuştur. Koagülaz testi, S. aureus izolatlarının tamamının koagülaz pozitif olduğunu göstermiştir. Disk difüzyon testi, izolatların tamamının (% 100) kloramfenikole ve teikoplanine duyarlı, penisilin G’ye ise dirençli olduğunu göstermiştir. İzolatların % 81.25’inin (13/16) metisilin dirençli S. aureus (MRSA) olduğu tespit edilmiştir. PZR işlemi sonucu S. aureus suşlarında en sık rastlanan antibiyotik direnç geninin blaZ (% 62.5, 10/16) olduğu tespit edilmiştir. S. aureus suşlarının hiçbirinde sea, seb, sec, sed ve see geni varlığı belirlenmemiştir.
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Primary Language tr
Subjects Food Science and Technology
Journal Section Articles
Authors

Orcid: 0000-0001-9209-1701
Author: Çilem KISA
Institution: SÜLEYMAN DEMİREL ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ, GIDA MÜHENDİSLİĞİ BÖLÜMÜ
Country: Turkey


Orcid: 0000-0002-2075-5027
Author: Yasin TUNCER (Primary Author)
Institution: Süleyman Demirel Üniversitesi Mühendislik Fakültesi
Country: Turkey


Supporting Institution Süleyman Demirel Üniversitesi
Project Number FYL-2019-6994
Thanks Bu çalışmayı FYL-2019-6994 nolu proje ile maddi olarak destekleyen Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi’ne teşekkür ederiz.
Dates

Publication Date : May 15, 2021

Bibtex @research article { gida893135, journal = {Gıda}, issn = {1300-3070}, eissn = {1309-6273}, address = {}, publisher = {Gıda Teknolojisi Derneği}, year = {2021}, volume = {46}, pages = {692 - 706}, doi = {10.15237/gida.GD21048}, title = {KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ}, key = {cite}, author = {Kısa, Çilem and Tuncer, Yasin} }
APA Kısa, Ç , Tuncer, Y . (2021). KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ . Gıda , 46 (3) , 692-706 . DOI: 10.15237/gida.GD21048
MLA Kısa, Ç , Tuncer, Y . "KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ" . Gıda 46 (2021 ): 692-706 <https://dergipark.org.tr/en/pub/gida/issue/61205/893135>
Chicago Kısa, Ç , Tuncer, Y . "KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ". Gıda 46 (2021 ): 692-706
RIS TY - JOUR T1 - KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ AU - Çilem Kısa , Yasin Tuncer Y1 - 2021 PY - 2021 N1 - doi: 10.15237/gida.GD21048 DO - 10.15237/gida.GD21048 T2 - Gıda JF - Journal JO - JOR SP - 692 EP - 706 VL - 46 IS - 3 SN - 1300-3070-1309-6273 M3 - doi: 10.15237/gida.GD21048 UR - https://doi.org/10.15237/gida.GD21048 Y2 - 2021 ER -
EndNote %0 Gıda KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ %A Çilem Kısa , Yasin Tuncer %T KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ %D 2021 %J Gıda %P 1300-3070-1309-6273 %V 46 %N 3 %R doi: 10.15237/gida.GD21048 %U 10.15237/gida.GD21048
ISNAD Kısa, Çilem , Tuncer, Yasin . "KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ". Gıda 46 / 3 (May 2021): 692-706 . https://doi.org/10.15237/gida.GD21048
AMA Kısa Ç , Tuncer Y . KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ. GIDA. 2021; 46(3): 692-706.
Vancouver Kısa Ç , Tuncer Y . KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ. Gıda. 2021; 46(3): 692-706.
IEEE Ç. Kısa and Y. Tuncer , "KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ", Gıda, vol. 46, no. 3, pp. 692-706, May. 2021, doi:10.15237/gida.GD21048