Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels

Year 2008, Volume: 36 Issue: 4, 347 - 352, 01.12.2008

Nazlı Sökmen Ferah Bican Fatma Ayhan Hakan Ayhan

Abstract

In this study, controlled drug release devices of poly ethylene glycol-diacrylate PEG-DA hydrogels were preparedby free radical UV polymerization. PEG-DA macromere was cross-linked with different percentages of ethylene glycoldimethacrylate EGDMA . The chelating agent, ethylene diamine tetraacetic acid EDTA , was added at three differentconcentrations. Gentamicin sulphate was incorporated into the hydrogel during photopolymerization and its releasekinetics was tested spectrophotometrically at 255 nm in phosphate buffer pH 7.4 . Results indicate that the presenceof chelating agent leads to a prolonged drug release. Gentamicin release from hydrogel synthesized by using lowchelating and cross-linking agent concentrations indicate slow release time. The effect of chelating agentconcentration eliminated when the amount of cross-linking agent increased while drug release time is still higher thancontrol group which was prepared in the absence of EDTA. It is interesting to note that the gentamicin release timeprolonged when chelating agent decreased at constant cross-linking agent concentration

Keywords

Chelating agent, PEG-DA, ethylene diamine tetraacetic acid EDTA, Hydrogel

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