Tribulus terrestris ekstraktının ilavesiyle gökkuşağı alabalığının (Oncorhynchus mykiss) sperm motilitesinin arttırılması

Year 2022, Volume: 8 Issue: 2, 397 - 404, 31.12.2022

Abdullatif Ölçülü Filiz Kutluyer Kocabaş Önder Aksu Mehmet Kocabaş Olcay Kaplan İnce Emrah Karakavuk

https://doi.org/10.29132/ijpas.1092956

Abstract

Tribulus terrestris (TT), Zygophyllaceae familyasına ait geleneksel bir bitkidir ve dünyada yaygın olarak dağılım gösterir. TT, testosteron, testosteron pekürsörü ve luteinize edici hormon seviyelerini yükseltme potansiyeline sahiptir. Bu çalışmada, Tribulus terrestris ilavesinin gökkuşağı alabalığının (Oncorhynchus mykiss) sperm motilitesi üzerindeki etkisini değerlendirmek için ilk kez denemeler yapılmıştır. Denemede farklı konsantrasyonlarda [0 µg L-1 (Kontrol), 200 µg L-1, 400 µg L-1, 600 µg L-1, 800 µg L-1 ve 1000 µg L-1] T. terrestris ekstraktı kullanılmıştır. Sperm motilite özellikleri ve süresi belirlenmiştir. Ayrıca T. terrestris ekstraktının protodioscin içeriği değerlendirilmiştir. Bu çalışma, T. terrestris varlığının sperm motilitesinde artışa neden olduğunu ortaya koymuştur. 400 µg L-1'de süre (49,00±3,61 s) ve motilite oranındaki (%91,67±2,89) artışlar istatistiksel olarak anlamlıydı (p<0.05). Sonuç olarak, aktivasyon solüsyonuna T. terrestris eklenmesi gökkuşağı alabalığının sperm motilitesini artırabilir.

Keywords

Bitkisel ürün, Oncorhynchus mykiss, spermatozoa, Tribulus terrestris

Enhancing motility of rainbow trout (Oncorhynchus mykiss) sperm by Tribulus terrestris extract supplementation

Abstract

Tribulus terrestris (TT) is a famous traditional plant of family Zygophyllaceae and widely distributes around the world. TT has potential to elevate the testosterone, testosterone precursor and luteinizing hormone levels. In this study, trials were conducted to assessment the impact of Tribulus terrestris addition on sperm motility of rainbow trout (Oncorhynchus mykiss) for the first time. In the trial, we used to different concentrations [0 µg L-1 (Control), 200 µg L-1, 400 µg L-1, 600 µg L-1, 800 µg L-1 and 1000 µg L-1] of T. terrestris extract. Sperm motility characteristics and longevity were determined. In addition, protodioscin content of T. terrestris extract was assessed. The present study revealed that the presence of T. terrestris caused to increase in sperm motility. The increases in duration (49.00±3.61 s) and motility rate (91.67±2.89%) at 400 µg L-1 were statistically significant (p<0.05). Overall, addition of T. terrestris to activation solution can increase the sperm motility of rainbow trout.

Keywords

Herbal medicine, Oncorhynchus mykiss, spermatozoa, Tribulus terrestris

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