Tribulus terrestris ekstraktının ilavesiyle gökkuşağı alabalığının (Oncorhynchus mykiss) sperm motilitesinin arttırılması

Yıl 2022, Cilt: 8 Sayı: 2, 397 - 404, 31.12.2022

Abdullatif Ölçülü , Filiz Kutluyer Kocabaş , Önder Aksu , Mehmet Kocabaş , Olcay Kaplan İnce , Emrah Karakavuk

https://doi.org/10.29132/ijpas.1092956

Öz

Tribulus terrestris (TT), Zygophyllaceae familyasına ait geleneksel bir bitkidir ve dünyada yaygın olarak dağılım gösterir. TT, testosteron, testosteron pekürsörü ve luteinize edici hormon seviyelerini yükseltme potansiyeline sahiptir. Bu çalışmada, Tribulus terrestris ilavesinin gökkuşağı alabalığının (Oncorhynchus mykiss) sperm motilitesi üzerindeki etkisini değerlendirmek için ilk kez denemeler yapılmıştır. Denemede farklı konsantrasyonlarda [0 µg L-1 (Kontrol), 200 µg L-1, 400 µg L-1, 600 µg L-1, 800 µg L-1 ve 1000 µg L-1] T. terrestris ekstraktı kullanılmıştır. Sperm motilite özellikleri ve süresi belirlenmiştir. Ayrıca T. terrestris ekstraktının protodioscin içeriği değerlendirilmiştir. Bu çalışma, T. terrestris varlığının sperm motilitesinde artışa neden olduğunu ortaya koymuştur. 400 µg L-1'de süre (49,00±3,61 s) ve motilite oranındaki (%91,67±2,89) artışlar istatistiksel olarak anlamlıydı (p<0.05). Sonuç olarak, aktivasyon solüsyonuna T. terrestris eklenmesi gökkuşağı alabalığının sperm motilitesini artırabilir.

Anahtar Kelimeler

Bitkisel ürün, Oncorhynchus mykiss, spermatozoa, Tribulus terrestris

Enhancing motility of rainbow trout (Oncorhynchus mykiss) sperm by Tribulus terrestris extract supplementation

Öz

Tribulus terrestris (TT) is a famous traditional plant of family Zygophyllaceae and widely distributes around the world. TT has potential to elevate the testosterone, testosterone precursor and luteinizing hormone levels. In this study, trials were conducted to assessment the impact of Tribulus terrestris addition on sperm motility of rainbow trout (Oncorhynchus mykiss) for the first time. In the trial, we used to different concentrations [0 µg L-1 (Control), 200 µg L-1, 400 µg L-1, 600 µg L-1, 800 µg L-1 and 1000 µg L-1] of T. terrestris extract. Sperm motility characteristics and longevity were determined. In addition, protodioscin content of T. terrestris extract was assessed. The present study revealed that the presence of T. terrestris caused to increase in sperm motility. The increases in duration (49.00±3.61 s) and motility rate (91.67±2.89%) at 400 µg L-1 were statistically significant (p<0.05). Overall, addition of T. terrestris to activation solution can increase the sperm motility of rainbow trout.

Anahtar Kelimeler

Herbal medicine, Oncorhynchus mykiss, spermatozoa, Tribulus terrestris

Kaynakça

• Adaay, MH. and Mattar, AG. (2012). Effect of aqueous and ethanolic extracts of Tribulus terrestris, Phoenix dactylifera and Nasturtium officinale mixture on some reproductive parameters in male mice. Baghdad Science Journal, 9, 640-650.
• Ahmed, I., Islam, M., Arshad, W. (2009). High-quality plant DNA extraction for PCR: an easy approach. Journal of Applied Genetics, 50, 105–107.
• Akram, M., Asif, HM., Akhtar, N., Shah, PA., Uzair, M., Shaheen, G., Shamim, T., Ali Shah, SM. and Ahmad, K. (2011). Tribulus terrestris Linn.: a review article. Journal of Medicinal Plants Research, 5, 3601-3605.
• Asadmobini, A., Bakhtiari, M., Khaleghi, S., Esmaeili, F. and Mostafaei A. (2017). The effect of Tribulus terrestris extract on motility and viability of human sperms after cryopreservation. Cryobiology, 75, 154-159.
• Basaiyye, SS., Naoghare, PK., Kanojiya, S., Bafana, A., Arrigo, P., Krishnamurthi, K. and Sivanesan, S. (2017). Molecular mechanism of apoptosis induction in Jurkat E6-1 cells by Tribulus terrestris alkaloids extract. Journal of Traditional and Complementary Medicine, Xxx, 1-10.
• Cek, S., Turan, F. (2007). Masculinization of African catfish (Clarias gariepinus) treated with gokshura (Tribulus terrestris). Israeli Journal of Aquaculture-Bamidgeh, 59, 20528.
• Cek, Ş., Turan, F., Atik, E. (2007). Masculinization of convict cichlid (Cichlasoma nigrofasciatum) by immersion in Tribulus terrestris extract. Aquaculture International, 15(2), 109-119.
• Fatima, L. and Sultana, A. (2017). Efficacy of Tribulus terrestris L. (fruits) in menopausal transition symptoms: A randomized placebo controlled study. Advances in Integrative Medicine, 4, 56-65.
• Fernández-Lázaro, D., Fernandez-Lazaro, CI., Seco-Calvo, J., Garrosa, E., Adams, DP. MielgoAyuso, J. (2022). Effects of Tribulus terrestris L. on Sport and Health Biomarkers in Physically Active Adult Males: A Systematic Review. International Journal of Environmental Research and Public Health, 19, 9533.
• Gauthaman, K., Adaikan, P. and Prasad, R. (2002). Aphrodisiac properties of Tribulus terrestris extract (Protodioscin) in normal and castrated rats. Life Sciences, 71 (12), 1385-1396. http://dx.doi.org/10.1016/S0024-3205(02)01858-1.
• Gauthaman, K. and Ganesan, AP. (2008). The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction – an evaluation using primates, rabbit and rat. Phytomedicine, 15, 44-54.
• Ganzera, M., Bedir, E. and Khan, IA. (2001). Determination of steroidal saponins in Tribulus terrestris by Reversed-Phase High-Performance Liquid Chromatography and Evaporative Light Scattering Detection. Journal of Pharmaceutical Sciences, 90, 11.
• Giovanelli, G. and Buratti, S. (2009). Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties. Food Chemistry, 112, 903-908.
• Gültepe, N., Acar, Ü., Kesbiç, O. S., Yılmaz, S., Yıldırım, Ö. and Türker, A. (2014). Effects of dietary Tribulus terrestris extract supplementation on growth, feed utilization, hematological, immunological, and biochemical variables of Nile tilapia Oreochromis niloticus. The Israeli Journal of Aquaculture-Bamidgeh, DOI: 10.46989/001c.20774.
• Hammoda, HM., Ghazy, NM., Harraz, FM., Radwan, MM., ElSohly, MA. and Abdallah II. (2013). Chemical constituents from Tribulus terrestris and screening of their antioxidant activity. Phytochemistry, 92, 153-159.
• Hassona, NN., Zayed, MM., Eltras, WF. and Mohamed, RA. (2020). Dietary supplementation of Tribulus terrestris extract improves growth and reproductive performances of the male Nile tilapia (Oreochromis niloticus). Aquaculture Research, 51(10), 4245-4254.
• Kamenov, Z., Fileva, S., Kalinov, K. and Jannini, EA. (2017). Evaluation of the efficacy and safety of Tribulus terrestris in malesexual dysfunction-A prospective, randomized, double-blind, placebo-controlled clinical trial. Maturitas, 99, 20-26.
• Kavitha, P., Subramanian, P. (2011). Effect of Tribulus terrestris on monosex production in Poecilia latipinna. Current Science, 100-104.
• Keshtmand, Z., Oryan, S., Ghanbari, A., Khazaei, M., Keshtmand, Z. and Oryan, S. (2014). Protective effect of Tribulus terrestris hydroalcoholic extract against cisplatin induced cytotoxicity on sperm parameters in male mice. International Journal of Morphology, 32, 551-557.
• Khaleghi, S., Bakhtiari, M., Asadmobini, A. and Esmaeili, F. (2017). Tribulus terrestris extract improves human sperm parameters in vitro. Evidence-Based Complementary and Alternative Medicine, 22 (3), 407-412.
• Kocabas, M. and Kutluyer, F. (2017a). In vitro effect of zinc: Evaluation of the sperm quality of endangered trout Salmo coruhensis and rainbow trout Oncorhynchus mykiss and fertilizing capacity. International Journal of Aquaculture and Fishery Sciences, 3 (2), 046-050.
• Kocabas, M., and Kutluyer, F. (2017b). Effect of Cobalt on sperm motility in an endangered trout species, Salmo coruhensis. Bulletin of Environmental Contamination and Toxicology, 99(6), 690-694.
• Kocabas, M., and Kutluyer F. (2017c). Dose dependent treatment with boric acid induces more changes in the sperm cells of endangered trout Salmo coruhensis and rainbow trout Oncorhynchus mykiss. Journal of Scientific and Engineering Research, 4 (9), 475-481.
• Kumar, P. and Singh, P. (2015). Tribulus terrestris ameliorates metronidazole induced spermatogenic inhibition and testicular oxidative stress in the laboratory mouse. Indian Journal of Pharmacology, 47(3), 304-310.
• Kumari, M. and Singh, P. (2015). Protective role of Tribulus terrestris on aluminium chloride-induced reproductive toxicity in the male laboratory mouse. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6), 2395-2405.
• Kutluyer, F. and Kocabas, M. (2017). Dose dependent treatment with boric acid induces more changes in the sperm cells of endangered Anatolian trout Salmo rizeensis. International Journal of Aquaculture and Fishery Sciences, 3(2), 042-045.
• Liu, J., Liang, P., Yin, C., Wang, T., Li, H. and Li, Y. 2004. Effects of several Chinese herbal aqueous extracts on human sperm motility in vitro. Andrologia, 36, 78-83.
• Ma, Y., Guo, Z. and Wang, X. (2017). Tribulus terrestris extracts alleviate muscle damage and promote anaerobic performance of trained male boxers and its mechanisms: Roles of androgen, IGF-1, and IGF binding protein-3. Journal of Sport and Health Science, 6, 474-481.
• Manach, C., Williamson, G., Morand, C., Scalbert, A. and Remesy, C. (2005). Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr., 81 (1 suppl), 230-242.
• Nassar, A., Mahony, M., Blackmore, P., Morshedi, M., Ozgur, K. and Oehninger, S. (1998). Increase of intracellular calcium is not a cause of pentoxifylline-induced hyperactivated motility or acrosome reaction in human sperm. Fertility and Sterility, 69, 748-754.
• Neychev, V. and Mitev, V. (2016). Pro-sexual and androgen enhancing effects of Tribulus terrestris L.: fact or fiction. Journal of Ethnopharmacology, 179, 345-355.
• Oliveira, NNPM., Félix, MAR., Pereira, TCS., Rocha, LGP., Miranda, JR., and Zangeronimo, MG. (2015). Sperm quality and testicular histomorphometry of wistar rats supplemented with extract and fractions of fruit of Tribulus terrestris L. Brazilian Archives of Biology and Technology, 58(6), 891-897.
• Omitoyin, B.O., Ajani, E.K. and Sadiq, H.O. (2013). Preliminary investigation of Tribulus terrestris (Linn., 1753) extracts as natural sex reversal agent in Oreochromis niloticus (Linn., 1758) larvae. International Journal of Aquaculture. 3 (23), 133-137.
• Rurangwa, E., Kime, D.E., Ollevier, F. and Nash, J.P. (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234, 1-28.
• Salgado, R.M., Marques-Silva, M.H., Gonçalves, E., Mathias, A.C., Aguiar, J.G. and Wolff, P. (2016). Effect of oral administration of Tribulus terrestris extract on semen quality and body fat index of infertile men. Andrologia, 49, e12655.
• Shishovska, M., Arsova-Sarafinovska, Z. and Memeti S. (2018). A simple method for determination of protodioscin in Tribulus terrestris L. and pharmaceuticals by high-performance liquid chromatography using diode-array detection. Journal of Chemical Engineering Research Updates, 2, 12-21.
• Singh, S., Nair, V. and Gupta, YK. (2012). Evaluation of the aphrodisiac activity of Tribulus terrestris Linn. in sexually sluggish male albino rats. Journal of Pharmacology and Pharmacotherapeutics, 3, 43-47.
• Yeganeh, S., Sotoudeh, A. and Movaffagh, AN. (2017). Effects of Tribulus terrestris extract on growth and reproductive performance of male convict cichlid (Cichlasoma nigrofasciatum). Turkish Journal of Fisheries and Aquatic Sciences, 17, 1003-1007.
• Yilmaz, S., Ergün, S., Kaya, H., Gürkan, M. (2014). Influence of Tribulus terrestris extract on the survival and histopathology of Oreochromis mossambicus (Peters, 1852) fry before and after Streptococcus iniae infection. Journal of Applied Ichthyology, 30(5), 994-1000.