Neuroprotective Effects of Rutin and Quercetin Flavonoids in Glaucium corniculatum Methanol and Water Extracts

Year 2017, Volume: 4 Issue: 3, Special Issue 1, 85 - 93, 25.11.2017

Fatma Gonca Koçancı , Belma Aslim

https://doi.org/10.21448/ijsm.363347

Cited By: 8

Abstract

Neurodegenerative diseases (NDs) are characterized by loss of neurons. NDs are associated with development of inflammation. Existing drugs against NDs only delay the progression; however, they do not provide a cure. The studies for the treatment of NDs focused on to discover natural products that have the potential of anti-inhibition and anti-radical properties. The Papaveraceae family members are important for the synthesis of pharmaceutically compounds such as flavonoids which act like anti-inflammatory drugs. In this study, methanol and water extracts of Glaucium corniculatum, a member of the Papaveraceae family, were analysed for flavonoid compounds. The effects of extracts on neuronal PC12 cells viability was determined. The anti-inflammatory effects of extracts were assessed by measuring the levels of IL-6 and IL-10 cytokines on hydrogen peroxide (H2O2)-stimulated PC12 cells. As a result of our studies, Rutin and Quercetin flavonoids have been found to be as major. The amount of Rutin was higher in methanol (45 μg/ml) than water (41 μg/ml). Quercetin was better extracted with methanol (12 μg/ml) than water (10 μg/ml). None of the tested extracts were cytotoxic even to PC12 cells. Both extracts showed an anti-inflammatory effect in a dose dependent manner. The water extract showed the maximum anti-inflammatory effect, with IL-6 secretion decreased 79 fold according to the H2O2 treated group and IL-10 secretion increased to 87 fold according to the control group. This study is an evidence that the Rutin and Quercetin flavonoids detected in G. corniculatum methanol and water extracts have a neuroprotective effect through anti-inflammation.

Keywords

Glaucium corniculatım, flavonoid, neurodegenerative diseases, anti-inflammation

Neuroprotective Effects of Rutin and Quercetin Flavonoids in Glaucium corniculatum Methanol and Water Extracts

Abstract

Neurodegenerative
diseases (NDs) are characterized by loss of neurons. NDs are associated with development
of inflammation. Existing drugs against NDs only delay the progression; however,
they do not provide a cure. The studies for the treatment of NDs focused on to discover
natural products that have the potential of anti-inhibition and anti-radical properties.
The Papaveraceae family members are important
for the synthesis of pharmaceutically compounds such as flavonoids which act like
anti-inflammatory drugs. In this study, methanol and water extracts of Glaucium corniculatum, a member of the Papaveraceae family, were analysed for flavonoid
compounds. The effects of extracts on neuronal PC12 cells viability was determined.
The anti-inflammatory effects of extracts were assessed by measuring the levels
of IL-6 and IL-10 cytokines on hydrogen peroxide (H₂O₂)-stimulated
PC12 cells. As a result of our studies, Rutin and Quercetin flavonoids have been
found to be as major. The amount of Rutin was higher in methanol (45 μg/ml) than
water (41 μg/ml). Quercetin was better extracted with methanol (12 μg/ml) than water
(10 μg/ml). None of the tested extracts were cytotoxic even to PC12 cells. Both
extracts showed an anti-inflammatory effect in a dose dependent manner. The water
extract showed the maximum anti-inflammatory effect, with IL-6 secretion decreased
79 fold according to the H₂O₂ treated group and IL-10 secretion
increased to 87 fold according to the control group. This study is an evidence that
the Rutin and Quercetin flavonoids detected in G. corniculatum methanol and water extracts have a neuroprotective effect
through anti-inflammation.

Keywords

Glaucium corniculatım, flavonoid, neurodegenerative diseases, anti-inflammation

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