Research Article

Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-sweet Whey Beverage

Volume: 9 Number: 4 December 26, 2025

Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-sweet Whey Beverage

Abstract

The underutilization of waste sweet whey in Zimbabwe poses a threat to the environment and results in high waste disposal costs to cheese processors. This study explores the reduction of waste sweet whey through development of a “cultured cereal sweet whey beverage (CSWb)” as a means of sustainable waste utilization. In this study, the effect of sweet whey inclusion on the nutritional, physicochemical, microbial and sensory attributes of CSWb was investigated. Treatments (T1-T4) containing varying concentration of cereal porridge (CP) and sweet whey (SW) were prepared. Treatments were T1 -20% SW + 67.7% CP; T2 -40.0% SW + 45.7% CP, T3 -50% SW + 35.7% CP, T4 -60% SW + 25.6% CP and control treatment with 0% SW + 85.8% CP. Data on the physicochemical properties (pH, ºBrix, lactic acid, consistency), nutrition (crude protein, fiber, phosphorous and calcium) and microbial quality (yeast and molds) was collected and analysed using RStudio ver. 4.5.0-2. Consumer acceptability dynamics were determined using mean ranks and PCA in Stata/MP ver. 19. Increasing the sweet whey to optimum concentration of 40% resulted in a nutritious CSWb with high content of crude protein, phosphorus and calcium. pH and lactic acid concentration increased (p < 0.05) with increasing sweet whey inclusion. Total soluble solids (ºBrix) showed a decrease (p < 0.05) as the concentration of sweet whey increased. Consistency values showed that the beverage became significantly thinner (p < 0.05) as sweet whey concentrations increased. Storage time significantly affected (p < 0.05) lactic acid, pH, Brix and consistency. The microbial quality fell within the recommended levels. Low values of yeast, molds and coliforms were observed over a storage time of 20 days, suggesting product microbial stability. Consumer studies showed that enhancing the CSWb with moderate percentages of sweet whey, up to 40%, improved its acceptability.

Keywords

Sweet whey, Nutritious cereal beverages, Sustainable waste utilization, Sensory Analysis, Physicochemical properties, Microbial quality

Supporting Institution

This research was supported by the University of Zimbabwe Department of Livestock Sciences.

Thanks

We appreciate Dr. J. A. Urombo (Harare Institute of Technology, Mathematical Sciences Department) for their advisory input during the progression of this study.

References

  1. Abdelhakam, K. E. K., Sid Ahmed, S. M. F., Farahat, F. H. O., Bushara, A. M. M., Ali, E. M. Y., Mohamed, A. A. (2022). Physicochemical properties, microbial load, and sensory attributes of sweet and sour whey of white cheese. Journal of the Saudi Society for Food and Nutrition, 15(1), 57-66. https://doi.org/10.9734/JALSI/2017/3476.
  2. AbdulAlim, T.S., Zayan, A.F., Campelo, P.H., Bakry, A.M. (2018). Development of new functional fermented product: mulberry-whey beverage. Journal of Nutrition, Food Research and Technology, 1(3), pp. 64–69. https://doi.org/10.30881/jnfrt.00013.
  3. Adebo, O.A., Gabriela Medina-Meza, I. (2020). Impact of Fermentation on the Phenolic Compounds and Antioxidant Activity of Whole Cereal Grains: A Mini Review. Molecules, 25(4), 927. https://doi.org/10.3390/molecules25040927.
  4. Akwasiam, B.U., Efiuvwevwere, B.J.O., Eruteya, O.C. (2023). Chemical composition, sensory and microbial analysis of yoghurt-like product from breadfruit, coconut and soybean extract. Scientia Africana, 22(2), 17–26. https://doi.org/10.4314/sa.v22i2.3.
  5. Alwohaibi, A. A., Ali, A. A., Sakr, S. S., Mohamed Ahmed, I. A., Alhomaid, R. M., Alsaleem, K. A., Aladhadh, M., Barakat, H., Hassan, M. F. (2023). Valorization of Different Dairy By-Products to Produce a Functional Dairy–Millet Beverage Fermented with Lactobacillus paracasei as an Adjunct Culture. Fermentation, 9(11), 927. https://doi.org/10.3390/fermentation9110927.
  6. Awobusuyi, T. D., Siwela, M., Kolanisi, U., & Amonsou, E. O. (2016). Provitamin A retention and sensory acceptability of amahewu, a non-alcoholic cereal-based beverage made with provitamin A-biofortified maize. Journal of the Science of Food and Agriculture, 96(4), 1356-1361. https://doi.org/10.1002/jsfa.7230.
  7. Barba, F.J. (2021). An Integrated Approach for the Valorization of Cheese Whey. Foods, 10(3), 564. https://doi.org/10.3390/foods10030564.
  8. Bhagat, Y.V. (2023) Enhancing Flavor and Appearance of Whey Beverages. Master’s Thesis. Cornell University, Faculty of the Graduate School, College of Agriculture and Life Science, New York, 10-32. https://ecommons.cornell.edu/bitstream/1813/113304/1/Bhagat_Yashashree_Project.pdf.
  9. Birania, S., Rohilla, P., Kumar, R., Saini, P. (2021). Whey and its valorization using Biorefinary: A review. Journal of Pharmacognosy and Phytochemistry, 10(1), 542–548. https://www.academia.edu/download/76285456/S-10-1-73-192.pdf.
  10. Buchanan, D., Martindale, W., Romeih, E., Hebishy, E. (2023). Recent advances in whey processing and valorisation: Technological and environmental perspectives. International Journal of Dairy Technology, 76(2), 291-312. https://doi.org/10.1111/1471-0307.12935.
APA
Nyagato, R. D., Hamandishe, V., Muyambo, S., Mutibvu, T., & Saidi, P. T. (2025). Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-sweet Whey Beverage. International Journal of Agriculture Environment and Food Sciences, 9(4), 1316-1329. https://doi.org/10.31015/2025.4.32
AMA
1.Nyagato RD, Hamandishe V, Muyambo S, Mutibvu T, Saidi PT. Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-sweet Whey Beverage. int. j. agric. environ. food sci. 2025;9(4):1316-1329. doi:10.31015/2025.4.32
Chicago
Nyagato, Rutendo Daisy, Vimbai Hamandishe, Shadreck Muyambo, Tonderai Mutibvu, and Petronella T. Saidi. 2025. “Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-Sweet Whey Beverage”. International Journal of Agriculture Environment and Food Sciences 9 (4): 1316-29. https://doi.org/10.31015/2025.4.32.
EndNote
Nyagato RD, Hamandishe V, Muyambo S, Mutibvu T, Saidi PT (December 1, 2025) Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-sweet Whey Beverage. International Journal of Agriculture Environment and Food Sciences 9 4 1316–1329.
IEEE
[1]R. D. Nyagato, V. Hamandishe, S. Muyambo, T. Mutibvu, and P. T. Saidi, “Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-sweet Whey Beverage”, int. j. agric. environ. food sci., vol. 9, no. 4, pp. 1316–1329, Dec. 2025, doi: 10.31015/2025.4.32.
ISNAD
Nyagato, Rutendo Daisy - Hamandishe, Vimbai - Muyambo, Shadreck - Mutibvu, Tonderai - Saidi, Petronella T. “Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-Sweet Whey Beverage”. International Journal of Agriculture Environment and Food Sciences 9/4 (December 1, 2025): 1316-1329. https://doi.org/10.31015/2025.4.32.
JAMA
1.Nyagato RD, Hamandishe V, Muyambo S, Mutibvu T, Saidi PT. Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-sweet Whey Beverage. int. j. agric. environ. food sci. 2025;9:1316–1329.
MLA
Nyagato, Rutendo Daisy, et al. “Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-Sweet Whey Beverage”. International Journal of Agriculture Environment and Food Sciences, vol. 9, no. 4, Dec. 2025, pp. 1316-29, doi:10.31015/2025.4.32.
Vancouver
1.Rutendo Daisy Nyagato, Vimbai Hamandishe, Shadreck Muyambo, Tonderai Mutibvu, Petronella T. Saidi. Physicochemical, Microbial and Sensory Attributes of a Cultured Cereal-sweet Whey Beverage. int. j. agric. environ. food sci. 2025 Dec. 1;9(4):1316-29. doi:10.31015/2025.4.32