Year 2020, Volume 4 , Issue 4, Pages 450 - 457 2020-12-15

Callus induction and antibacterial activity of various explants of Indigofera zollingeriana

Siti MAESAROH [1] , Çiğdem Alev ÖZEL [2] , Hikmet KATIRCIOĞLU [3] , Nurdan ŞAHİN DEMİRBAĞ [4]

The study explore antibacterial potential of Indigofera zollingeriana a popular medicinal and forage plant of the tropics. Methanol extracts of calli from hypocotyl, cotyledon and leaf excised from in vitro-grown plants using different plant growth regulator and their combinations had significant reaction against Gram-positive Staphylococcus aureus ATTCC25923 and Gram-negative Pseudomonas aeruginosa ATCC25823 bacteria. The best antibacterial activity was noted on the callus extracts of hypocotyl explants with significant inhibition zones on MS medium containing 2 mg/L BAP + 0.1 mg/L NAA. The P. aeruginosa had maximum inhibition on 0.5 mg/L BAP + 0.1 mg/L NAA. This antibacterial activity (by the hypocotyl induced calli extracts) was higher compared to the antibacterial activity noted from the extracts of other two explants and non treated control treatments. It was concluded that antibacterial activities were affected by exlants source and plant growth regulators. The extracts from in vitro induced callus from hypocotyl, cotyledon and leaf explants of I.zollingeriana could be used variably and effectively against both type of bacteria used in this study. 
Callus, Extract, Legume, Medicinical, Phytohormone
  • Annathurai, K., Palaniyandi, K. and Raamaiah R. (2015) Callus induction and antimicrobial activity from Ziziphus mauritiana. Lam cotyledon kernels. Indian Journal of Applied Microbiology, 18, 2, 32-39.
  • Arafa, N.M., Mohamed, S.S. and Aly, U.I. (2016) In vitro antimicrobial activity of carrot callus extracts as affected by tyrosine and tryptophan precursor. International Journal of PharmTech Research 9, 9, 121-129.
  • Ashrafee, T.S., Rahman, M.M., Chakraborty, A. and Prodhan, S.H. (2014) Antibacterial potentiality of red sandalwood callus against pathogenic isolates of aeromonas and pseudomonas. Universal Journal of Plant Science, 2, 4, 86-91. DOI: 10.13189/ujps.2014.020402
  • Bakasso, S.A., Lamien-Meda, C.E., Lamien, M., Kiendrebeogo, J., Millogo, A.G. and Ouedraogo Nacoulma, O.G. (2008) Polyphenol contents and antioxidant activities of five Indigofera species (Fabaceae) from Burkina Faso. Pakistan Journal of Biological Sciences, 11, 1429-1435. DOI: 10.3923/pjbs.2008.1429.1435.
  • Basra, A. (2000). Plant Growth Regulators in Agriculture and Horticulture: Their Role and Commercial Uses. Taylor & Francis, CRC Press, USA, 264 pages.
  • Bisio, A., Fraternale, D., Schito, A.M., et al. (2016) Establishment and analysis of in vitro biomass from Salvia corrugate Vahl. and evaluation of antimicrobial activity. Phytochemistry, 122, 276-285. DOI: 10.1016/j.phytochem.2015.12.017
  • Borgatti, S.P. (1998). Elicitation techniques for cultural domain analysis. In: Ethnographer’s Toolkit, Schensul, J., LeCompte, M. (Eds). Altimira Press, Walnut Creek, CA, 1-2.
  • Chouhan, S., Sharma, K. and Guleria, S. (2017) Antimicrobial activity of some essential oils-present status and future perspectives. Medicines, 4, 3, 58. DOI: 10.3390/medicines4030058
  • Christie, G.S., Madsen, N.P. and Hegarty, M.P. (1969) Acute biochemical changes in rat liver induced by the naturally occurring amino acid indospicine. Biochemical Pharmacology, 18, 693-700. DOI: 10.1016/0006-2952(69)90039-2
  • Cohen, M.L. (1992) Epidemiology of drug resistance: implications for a post-antimicrobial era. Science, 257, 1050-1055. DOI: 10.1126/science.257.5073.1050
  • Dahot, M.U. (1999) Antibacterial and antifungal activity of small protein of Indifogera oblongifolia leaves. Journal of Ethnopharmacology, 64, 277-282. DOI: 10.1016/S0378-8741(98)00136-6
  • ElNour, M.E.M., Ali, A.M.A. and Saeed, B.E.A.E. (2015) Antimicrobial activities and phytochemical screening of callus and seeds extracts of fenugreek (Trigonella foenum-graecum). International Journal of Current Microbiology and Applied Sciences, 4, 2, 147-157.
  • Esimone, C.O., Adikwu, M.U. and Muko, K.N. (1999) Antimicrobial properties of Indigofera dendroides leaves. Fitoterapia, 70, 517-520. DOI: 10.1016/S0367-326X(99)00072-6
  • Frank, M., Rupp, H.M, Prinsen, E., Motyka, V., Van Onckelen, H. and Schmülling, T. (2000) Hormone autotrophic growth and differentiation identifies mutant lines of Arabidopsis with altered cytokinin and auxin content or signaling. Plant Physiology, 122, 721-729. DOI: 10.1104/pp.122.3.721.
  • Ghasemi, Y., Yazdi, M.T., Shokravi, S., et al. (2003) Antifungal and antibacterial activity of paddy fields Cyanobacteria from the North of Iran. Journal of Science, Islamic Republic of Iran, 14, 203-209.
  • Goyal, S. and Ramawat, K.G. (2008) Synergistic effect of morphactin on cytokinin-induced production of isoflavonoids in cell cultures of Pueraria tuberosa (Roxb. ex. Willd.) DC. Plant Growth Regulation, 55, 175-181. DOI: 10.1007/s10725-008-9271-x
  • Hoffman, B. and Gallaher, T. (2007) Importance indices in Ethnobotany. Ethnobotany Research & Applications, 5, 201-218.
  • Inouye, S., Takizawa, T. and Yamaguchi, H. (2001) Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. The Journal of Antimicrobial Chemotherapy, 47, 5, 565-573. DOI: 10.1093/jac/47.5.565
  • Iwase, A., Mitsuda, N., Koyama, T., et al. (2011) The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis. Current Biology, 21, 508-514. DOI: 10.1016/j.cub.2011.02.020
  • Jahan, N., Khatoon, R., Shahzad, A., Shahid, M. and Ahmad, S. 2013. Comparison of antibacterial activity of parent plant of Tylophora indica Merr. with its in vitro raised plant and leaf callus. African Journal of Biotechnology, 12, 31, 4891-4896. DOI: 10.5897/AJB2013.12488
  • Jain, S.C., Pancholi, B. and Jain, R. (2012) In-vitro callus propagation and secondary metabolite quantification in Sericostoma pauciflorum. Iranian Journal of Pharmaceutical Research, 11, 4, 1103-1109. DOI: 10.22037/IJPR.2012.1202
  • Jansen, A.M., Cheffer, J.J.C. and Svendsen, A.B. (1987) Antimicrobial activity of essencial oils: a 1976-1986 literature review: Aspects of test methods. Planta Medica, 40, 395-398. DOI: 10.1055/s-2006-962755
  • Jeyakani Santhosh, M. and Rajalakshmi, M. (2016) Antimicrobial activities screening of callus and leaf extracts of tea tree (Melaluca alternifolia). World Journal of Pharmacy Pharmaceutical Sciences, 5, 9, 1155-1162.
  • Jisha, V.N. and Benjamin, S. (2009) Micropropogation and anti microbial activity studies on Indigofera tinctoria. Journal of Tropical Medicinal Plants, 10, 1, 73-79.
  • Johnson, M. and Babu, A. (2010) Somoclonal variation studies on Passiflora mollussima (H.B.K.) Bailey using phytochemical methods. Natural Products An Indian Journal, 6, 1, 5-10.
  • Kumar, R.S., Moorthy, K., Vinodhini, R. and Punitha, T. (2013) Antimicrobial efficacy and phytochemical analysis of Indigofera trita Linn. African Journal of Traditional Complementary and Alternative Medicines, 10, 3, 518-525. DOI: 10.4314/ajtcam.v10i3.20
  • Leite, S.P., Leite, A.C.R., Paiva, P.M.G., Coelho, L.C.B.B., Lima, E.O. and Lima, V.L.M. 2002. Atividade Lectínica em folhas de Indigofera suffruticosa (Lectin activity in leaves from Indigofera suffruticosa). VI Reunião Regional da SBBq, Nordeste, Fortaleza Ceará Resumo Completo.
  • Leite, S.P., Vieira, J.R.C., Medeiros, P.L., Leite, R.M.P., Lima, V.L.M., Xavier, H.S. and Lima, E.O. (2006) Antimicrobial activity of Indigofera suffruticosa. Evidence-based Complementary and Alternative Medicine, 3, 2, 261-265. DOI: 10.1093/ecam/nel010
  • Medeiros, M.F.T., Silva, P.S. and Albuquerque, U.P. (2011) Quantification in ethnobotanical research: an overview of indices used from 1995 to 2009. Sitientibus série Ciências Biologicas, 11, 2, 211-230.
  • Mohajer, S., Taha, R.M., Khorasani, A. and Yaacob, J.S. (2012) Induction of different types of callus and somatic embryogenesis in various explants of Sainfoin (Onobrychis sativa). Australian Journal of Crop Science, 6, 8, 1305-1313.
  • Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 3, 473-497. DOI: 10.1111/j.1399-3054.1962.tn08052.x
  • Murthy, H.N., Lee, E.J. and Peak, K.Y. (2014) Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell, Tissue and Organ Culture, 118, 1-16. DOI: 10.1007/s11240-014-0467-7
  • Nascimento, G.G.F., Locatelli, J., Freitas, P.C. and Silva, G.L. (2000) Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology, 31, 4, 247-256. DOI: 10.1590/S1517-83822000000400003
  • Ngoci, S.N., Matasyoh, J.C., Mwaniki, C.G. and Mwendia, C.M. (2012) Antibacterial activity of methanol root extract of Indigofera lupatana Baker F. Eastern Journal of Medicine, 17, 1, 11-16.
  • Nisar, M., Tariq, S.A., Marwat, I.K., Shah, M.R. and Khan, I.A. (2009) Antibacterial, antifungal, insecticidal, cytotoxicity and phytotoxicity studies on Indigofera gerardiana. Journal of Enzyme Inhibition and Medicinal Chemistry, 24, 1, 224-229. DOI: 10.1080/14756360802051313
  • Perez, C., Paul, M. and Bazerque, P. (1990) An antibiotic assay by agar-well diffusion method. Acta Biologiae et Medicinae Experimentalis 15: 113-115.
  • Petrosyan, M., Shcherbakova, Y., Sahakyan, N., Vardanyan, Z., Poladyan, A., Popov, Y. and Trchounian, A. (2015) Alkanna orientalis (L.) Boiss. plant isolated cultures and antimicrobial activity of their extracts: phenomenon, dependence on different factors and effects on some membraneassociated properties of bacteria. Plant Cell, Tissue and Organ Culture, 122, 727-738. DOI: 10.1007/s11240-015-0806-3
  • Prabakaran, M., Chandrakala, N. and Panneerselvam, A. (2011) Antimicrobial activity of Indigofera glandulosa (wild). Asian Journal of Plant Science and Research, 1, 2, 18-25.
  • Praveen, R.P. and Nair, A. (2014) Comparative stufy on antimicrobial efficacy of methanolic extract of root, callusand fruit extracts of Myxopyrum Smilacifolium Blume. International Journal of Applied Sciences and Biotechnology, 2, 4, 521-524. DOI: 10.3126/ijasbt.v2i4.11362
  • Rajaperumal, S., Nimmi, M. and Ranjitha Kumari, B.D. (2013) In vitro studies on antimicrobial and antioxidant effect of methanolic extract of Indigofera aspalathoides (Vahl ex DC) and its cytotoxic property against human lung cancer cell line NCI H460. European Journal of Experimental Biology, 3, 3, 18-29.
  • Ranukadevi, K.P. and Suhani Sultana, S. (2011) Determination of antibacterial, antioxidant and cytotoxicity effect of Indigofera tinctoria on Lung Cancer Cell Line NCI-h69. International Journal of Pharmacology, 7, 3, 356-362. DOI: 10.3923/ijp.2011.356.362
  • Ravinder Singh, C. (2011) Antimicrobial effect of callus and natural plant extracts of Premna serratifolia L. International Journal of Pharmaceutical and Biomedical Research, 2, 1, 17-20.
  • Saikia, M., Shrivastava, K. and Singh, S.S. (2013) Effect of culture media and growth hormones on callus ınduction in Aquilaria malaccensis Lam., a medicinally and commercially ımportant tree species of North East India. Asian Journal of Biological Sciences, 6, 96-105. DOI: 10.3923/ajbs.2013.96.105
  • Santos, I.P., Silva, L.C.N., Silva, M.V., Araújo, J.M., Cavalcanti, M.S., and Lima, V.L.M. (2015) Antibacterial activity of endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae). Frontiers in Microbiology, 6, 350. DOI: 10.3389/fmicb.2015.00350
  • Sari, Y.P., Kusumawati, E., Saleh, C., Kustiawan, W. and Sukarningsih. 2018. Effect of sucrose and plant growth regulators on callogenesis and preliminary secondary metabolic of different explant Myrmecodia tuberosa. Nusantara Bioscience, 10, 3, 183-192. DOI: 10.13057/nusbiosci/n100309
  • Shrikhande, M., Thengane, S.R. and Mascarenhas, A.F. (1993) Somatic embryogenesis and plant regeneration in Azadirachta indica A. Juss. In Vitro Cellular & Developmental Biology-Plany, 29, 1, 38-42. DOI: 10.1007/BF02632237
  • Singh, B. and Sharma, R.A. (2015) Antioxidant and antimicrobial activities of callus culture and fruits of Phyllanthus emblica L. Journal of Herbs, Spices and Medicinal Plants, 21, 3, 230-242. DOI: 10.1080/10496475.2014.949998
  • Skoog, F. and Miller, C.O. (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposia of the Society for Experimental Biology, 11, 118-130.
  • Soorni, J. and Kahrizi, D. (2015) Effect of genotype, explant type and 2,4 D on cell dedifferentiation and callus induction in cumin (Cuminum cyminum L.) medical plant. Journal of Applied Biotechnology Reports, 2, 3, 265-270.
  • Staba, E.J. (1980). Plant tissue culture as a source of biochemicals. CRS Press, Boca Raton, Florida, 285 pages.
  • Spartz, A.K. and Gray, W.M. (2008) Plant hormone receptors: new perceptions. Genes and Development, 22, 16, 2139-2148. DOI: 10.1101/gad.1693208
  • Terras, F.R.G., Eggermont, K., Kovaleva, V. et al. (1995) Small cysteine rich antifungal proteins from radish: their role in host defence. Plant Cell, 7, 5, 573–588. DOI: 10.1105/tpc.7.5.573
  • Vaezi, Z., Daneshvar, M.H., Heidari, M. and Chehrazi, M. (2015) Indirect regeneration plant Fenugreek (Trigonella foenumgraecum L), with the use of plant growth regulators in vitro. Bulletin of Environment, Pharmacology and Life Sciences, 4, 5, 103-108.
  • Verpoorte, R., van der Heijden, R., van Gulik, W.M. and ten Hoopen, H.J.G. (1991). Plant biotechnology for the production of alkaloids: present status and prospects. In: The alkaloids: chemistry and pharmacology, Brossi, A. (ed), Vol 40. Academic Press, San Diego, 1-187
Primary Language en
Subjects Agriculture
Published Date December 2020
Journal Section Research Articles

Orcid: 0000-0003-1024-284X
Author: Siti MAESAROH (Primary Author)
Country: Turkey

Orcid: 0000-0002-5952-1412
Author: Çiğdem Alev ÖZEL
Institution: Gazı University
Country: Turkey

Author: Hikmet KATIRCIOĞLU
Institution: Gazı University
Country: Turkey

Country: Turkey


Application Date : August 21, 2020
Acceptance Date : October 26, 2020
Publication Date : December 15, 2020

APA Maesaroh, S , Özel, Ç , Katırcıoğlu, H , Şahi̇n Demi̇rbağ, N . (2020). Callus induction and antibacterial activity of various explants of Indigofera zollingeriana . International Journal of Agriculture Environment and Food Sciences , 4 (4) , 450-457 . DOI: 10.31015/jaefs.2020.4.8