Year 2020, Volume 4 , Issue 4, Pages 450 - 457 2020-12-15

Callus induction and antibacterial activity of various explants of Indigofera zollingeriana

Siti MAESAROH [1] , Çiğdem Alev ÖZEL [2] , Hikmet KATIRCIOĞLU [3] , Nurdan ŞAHİN DEMİRBAĞ [4]


The study explore antibacterial potential of Indigofera zollingeriana a popular medicinal and forage plant of the tropics. Methanol extracts of calli from hypocotyl, cotyledon and leaf excised from in vitro-grown plants using different plant growth regulator and their combinations had significant reaction against Gram-positive Staphylococcus aureus ATTCC25923 and Gram-negative Pseudomonas aeruginosa ATCC25823 bacteria. The best antibacterial activity was noted on the callus extracts of hypocotyl explants with significant inhibition zones on MS medium containing 2 mg/L BAP + 0.1 mg/L NAA. The P. aeruginosa had maximum inhibition on 0.5 mg/L BAP + 0.1 mg/L NAA. This antibacterial activity (by the hypocotyl induced calli extracts) was higher compared to the antibacterial activity noted from the extracts of other two explants and non treated control treatments. It was concluded that antibacterial activities were affected by exlants source and plant growth regulators. The extracts from in vitro induced callus from hypocotyl, cotyledon and leaf explants of I.zollingeriana could be used variably and effectively against both type of bacteria used in this study. 
Callus, Extract, Legume, Medicinical, Phytohormone
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Primary Language en
Subjects Agriculture
Published Date December 2020
Journal Section Research Articles
Authors

Orcid: 0000-0003-1024-284X
Author: Siti MAESAROH (Primary Author)
Institution: ANKARA ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-5952-1412
Author: Çiğdem Alev ÖZEL
Institution: Gazı University
Country: Turkey


Author: Hikmet KATIRCIOĞLU
Institution: Gazı University
Country: Turkey


Author: Nurdan ŞAHİN DEMİRBAĞ
Institution: ANKARA UNIVERSITY
Country: Turkey


Dates

Application Date : August 21, 2020
Acceptance Date : October 26, 2020
Publication Date : December 15, 2020

APA Maesaroh, S , Özel, Ç , Katırcıoğlu, H , Şahi̇n Demi̇rbağ, N . (2020). Callus induction and antibacterial activity of various explants of Indigofera zollingeriana . International Journal of Agriculture Environment and Food Sciences , 4 (4) , 450-457 . DOI: 10.31015/jaefs.2020.4.8