The study explore antibacterial potential of Indigofera zollingeriana a popular medicinal and forage plant of the tropics. Methanol extracts of calli from hypocotyl, cotyledon and leaf excised from in vitro-grown plants using different plant growth regulator and their combinations had significant reaction against Gram-positive Staphylococcus aureus ATTCC25923 and Gram-negative Pseudomonas aeruginosa ATCC25823 bacteria. The best antibacterial activity was noted on the callus extracts of hypocotyl explants with significant inhibition zones on MS medium containing 2 mg/L BAP + 0.1 mg/L NAA. The P. aeruginosa had maximum inhibition on 0.5 mg/L BAP + 0.1 mg/L NAA. This antibacterial activity (by the hypocotyl induced calli extracts) was higher compared to the antibacterial activity noted from the extracts of other two explants and non treated control treatments. It was concluded that antibacterial activities were affected by exlants source and plant growth regulators. The extracts from in vitro induced callus from hypocotyl, cotyledon and leaf explants of I.zollingeriana could be used variably and effectively against both type of bacteria used in this study.
Callus, Extract, Legume, Medicinical, Phytohormone