Research Article
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Year 2021, Volume: 11 Issue: 3, 1763 - 1769, 01.09.2021
https://doi.org/10.21597/jist.864028

Abstract

References

  • Calderon LA, Sobrinho JC, Zaqueo KD, de Moura AA, Grabner AN, Mazzi, MV, Marcussi S, Nomizo A, Fernandes CFC, Zuliani JP, Carvalho BMA, da Silva SL, Stábeli RG, Soares AM, 2014. Antitumoral activity of snake venom proteins: New Trends in Cancer Therapy. BioMed Research International, 2014: 1-19.
  • Er A, Corum O, Corum D, Hitit M, Donmez H, Guzeloglu A, 2017. Alcoholic extract of tarantula cubensis induces apoptosis in MCF-7 cell line. Biomedical Research, 28 (8): 3660-3665.
  • Estrada G, Villegas E, Corzo G, 2007. Spider venoms: A rich source of acylpolyamines and peptides as new leads for CNS drugs. Natural Product Reports, 24 (1): 145-161.
  • Fry BG, Wickramaratana JC, Lemme S, Beuve A, Garbers D, Hodgson WC, Alewood P, 2005. Novel natriuretic peptides from the venom of the inland taipan (Oxyuranus microlepidotus): isolation, chemical and biological characterisation. Biochemical and Biophysical Research Communications, 327 (4): 1011-1015.
  • Ghasemi-Dizgah A, Amirmozafari N, 2015. Evaluation of antibacterial effect of tarantula cubensis venome (Theranekron). International Journal of Biology, Pharmacy and Allied Sciences, 5980-5989.
  • Ghasemi-Dizgah A, Nami B, Amirmozafari N, 2017. Tarantula cubensis venom (theranekron ®) selectively destroys human cancer cells via activating caspase-3-mediated apoptosis. Acta Medica International, 4 (1): 74.
  • Gultiken N, Guvenc T, Kaya D, Agaoglu AR, Ay SS, Kucukaslan I, Emre B, Findik M, Schäfer-Somi S, Aslan S, 2015. Tarantula cubensis extract alters the degree of apoptosis and mitosis in canine mammary adenocarcinomas. Journal of Veterinary Science, 16 (2): 213-219.
  • Gultiken N, Vural MR, 2007. The effect of Tarantula cubensis extract applied in pre and postoperative period of canine mammary tumours. Journal of Istanbul Veterinary Sciences, (2), 13-23.
  • Ilhan S, Çiçek K, Tok CV, Atmaca H, 2020. Profiling of apoptosis-associated proteins in human prostate cancer cells in response to Montivipera bulgardaghica albizona venom by protein array. Toxin Reviews, 1-8.
  • King GF, 2011. Venoms as a platform for human drugs: Translating toxins into therapeutics. Expert Opinion on Biological Therapy. 11 (11): 1469-1484.
  • Oldrati V, Arrell M, Violette A, Perret F, Sprüngli X, Wolfender JL, Stöcklin R, 2016. Advances in venomics. Molecular BioSystems, 12 (12): 3530–3543.
  • Oldrati V, Bianchi E Stöcklin, R, 2013. Spider Venom Components as Drug Candidates. In Spider Ecophysiology (pp. 491-503). Springer Berlin Heidelberg.
  • Olivera BM, McIntosh JM, Curz LJ, Luque FA, Gray WR, 1984. Purification and sequence of a presynaptic peptide toxin from Conus geographus venom. Biochemistry, 23 (22): 5087-5090.
  • Saez NJ, Senff S, Jensen JE, Er SY, Herzig V, Rash LD, King GF, 2010. Spider-Venom Peptides as Therapeutics. Toxins, 2 (12): 2851-2871.
  • Vassilevski AA, Kozlov SA, Grishin EV, 2009. Molecular diversity of spider venom. Biochemistry (Moscow), 74 (13): 1505-1534.
  • Yenigun VB, Azzawri AA, Acar MS, Kaplan MB, Ucar VB, Tastekin D, Acar H, 2021. Alcoholic extract of Tarantula cubensis (Theranekron®) induce autophagy on gastric cancer cells. Experimental Biomedical Research, 4 (2):89-98.

Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells

Year 2021, Volume: 11 Issue: 3, 1763 - 1769, 01.09.2021
https://doi.org/10.21597/jist.864028

Abstract

Tarantula cubensis is known as Cuban tarantula having a venom that contains a diverse mixture of potent compounds with various biological activities. These peptides have been shown to have antitumor activities, therefore features of spider-venom peptides prompted scientists to test them as a potential anticancer drug. The purpose of the study was to investigate the potential cytotoxic effect of Tarantula cubensis venom (Logoplex®) on human cancer cells including prostate (PC-3), lung (H69), breast (MDA-MB-231), and ovarian (OVCAR-3). Moreover, non-tumorigenic MCF-10A cells were used to evaluate the possible cancer cell-specific effect of the extract. The increasing concentrations of Logoplex® were applied for 24, 48 and 72 h. MTT assay was used to assess cell viability. Concentration-response curves and the IC50 values were determined via Graphpad Prism software. Logoplex® caused a time- and concentration-dependent cytotoxic effect in MDA-MB-231, PC-3, OVCAR-3 and MCF-10A cells and the highest cytotoxicity was achieved at 72h. However, in H69 cells, there was a concentration-dependent cytotoxic effect and the highest cytotoxicity was achieved at 24h. IC50 values of Logoplex® in MDA-MB-231, OVCAR-3, PC-3, H69 and MCF-10A cells were determined as 159.3±2.1, 48.9±1.8, 40.2±1.2, 498.3±1.2 and 217.8±2.0 µg/mL, respectively. Logoplex® showed a lower cytotoxic effect against normal cells than the cancer cells suggesting a cancer cell-specific effect. According to the preliminary results of this study, although Logoplex® is a veterinary drug, its cytotoxic effect on human cancer cells suggests that it should be re-evaluated as a potential cytotoxic agent. Analyses to identify functional compounds of Tarantula cubensis venom, and future studies addressing its mechanism of action on cancer cells are recommended.

References

  • Calderon LA, Sobrinho JC, Zaqueo KD, de Moura AA, Grabner AN, Mazzi, MV, Marcussi S, Nomizo A, Fernandes CFC, Zuliani JP, Carvalho BMA, da Silva SL, Stábeli RG, Soares AM, 2014. Antitumoral activity of snake venom proteins: New Trends in Cancer Therapy. BioMed Research International, 2014: 1-19.
  • Er A, Corum O, Corum D, Hitit M, Donmez H, Guzeloglu A, 2017. Alcoholic extract of tarantula cubensis induces apoptosis in MCF-7 cell line. Biomedical Research, 28 (8): 3660-3665.
  • Estrada G, Villegas E, Corzo G, 2007. Spider venoms: A rich source of acylpolyamines and peptides as new leads for CNS drugs. Natural Product Reports, 24 (1): 145-161.
  • Fry BG, Wickramaratana JC, Lemme S, Beuve A, Garbers D, Hodgson WC, Alewood P, 2005. Novel natriuretic peptides from the venom of the inland taipan (Oxyuranus microlepidotus): isolation, chemical and biological characterisation. Biochemical and Biophysical Research Communications, 327 (4): 1011-1015.
  • Ghasemi-Dizgah A, Amirmozafari N, 2015. Evaluation of antibacterial effect of tarantula cubensis venome (Theranekron). International Journal of Biology, Pharmacy and Allied Sciences, 5980-5989.
  • Ghasemi-Dizgah A, Nami B, Amirmozafari N, 2017. Tarantula cubensis venom (theranekron ®) selectively destroys human cancer cells via activating caspase-3-mediated apoptosis. Acta Medica International, 4 (1): 74.
  • Gultiken N, Guvenc T, Kaya D, Agaoglu AR, Ay SS, Kucukaslan I, Emre B, Findik M, Schäfer-Somi S, Aslan S, 2015. Tarantula cubensis extract alters the degree of apoptosis and mitosis in canine mammary adenocarcinomas. Journal of Veterinary Science, 16 (2): 213-219.
  • Gultiken N, Vural MR, 2007. The effect of Tarantula cubensis extract applied in pre and postoperative period of canine mammary tumours. Journal of Istanbul Veterinary Sciences, (2), 13-23.
  • Ilhan S, Çiçek K, Tok CV, Atmaca H, 2020. Profiling of apoptosis-associated proteins in human prostate cancer cells in response to Montivipera bulgardaghica albizona venom by protein array. Toxin Reviews, 1-8.
  • King GF, 2011. Venoms as a platform for human drugs: Translating toxins into therapeutics. Expert Opinion on Biological Therapy. 11 (11): 1469-1484.
  • Oldrati V, Arrell M, Violette A, Perret F, Sprüngli X, Wolfender JL, Stöcklin R, 2016. Advances in venomics. Molecular BioSystems, 12 (12): 3530–3543.
  • Oldrati V, Bianchi E Stöcklin, R, 2013. Spider Venom Components as Drug Candidates. In Spider Ecophysiology (pp. 491-503). Springer Berlin Heidelberg.
  • Olivera BM, McIntosh JM, Curz LJ, Luque FA, Gray WR, 1984. Purification and sequence of a presynaptic peptide toxin from Conus geographus venom. Biochemistry, 23 (22): 5087-5090.
  • Saez NJ, Senff S, Jensen JE, Er SY, Herzig V, Rash LD, King GF, 2010. Spider-Venom Peptides as Therapeutics. Toxins, 2 (12): 2851-2871.
  • Vassilevski AA, Kozlov SA, Grishin EV, 2009. Molecular diversity of spider venom. Biochemistry (Moscow), 74 (13): 1505-1534.
  • Yenigun VB, Azzawri AA, Acar MS, Kaplan MB, Ucar VB, Tastekin D, Acar H, 2021. Alcoholic extract of Tarantula cubensis (Theranekron®) induce autophagy on gastric cancer cells. Experimental Biomedical Research, 4 (2):89-98.
There are 16 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Biyoloji / Biology
Authors

Süleyman İlhan 0000-0002-6584-3979

Publication Date September 1, 2021
Submission Date January 18, 2021
Acceptance Date June 2, 2021
Published in Issue Year 2021 Volume: 11 Issue: 3

Cite

APA İlhan, S. (2021). Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells. Journal of the Institute of Science and Technology, 11(3), 1763-1769. https://doi.org/10.21597/jist.864028
AMA İlhan S. Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells. J. Inst. Sci. and Tech. September 2021;11(3):1763-1769. doi:10.21597/jist.864028
Chicago İlhan, Süleyman. “Can a Veterinary Drug Be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula Cubensis Venom on Human Cancer Cells”. Journal of the Institute of Science and Technology 11, no. 3 (September 2021): 1763-69. https://doi.org/10.21597/jist.864028.
EndNote İlhan S (September 1, 2021) Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells. Journal of the Institute of Science and Technology 11 3 1763–1769.
IEEE S. İlhan, “Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells”, J. Inst. Sci. and Tech., vol. 11, no. 3, pp. 1763–1769, 2021, doi: 10.21597/jist.864028.
ISNAD İlhan, Süleyman. “Can a Veterinary Drug Be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula Cubensis Venom on Human Cancer Cells”. Journal of the Institute of Science and Technology 11/3 (September 2021), 1763-1769. https://doi.org/10.21597/jist.864028.
JAMA İlhan S. Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells. J. Inst. Sci. and Tech. 2021;11:1763–1769.
MLA İlhan, Süleyman. “Can a Veterinary Drug Be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula Cubensis Venom on Human Cancer Cells”. Journal of the Institute of Science and Technology, vol. 11, no. 3, 2021, pp. 1763-9, doi:10.21597/jist.864028.
Vancouver İlhan S. Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells. J. Inst. Sci. and Tech. 2021;11(3):1763-9.