Karanfil Yağının Japon Balığının (Carassius auratus) Anestezisinde Optimal Konsantrasyonu ve Balığın Taşınmasında Stoklama Yoğunluğuna Etkisi
Yıl 2022,
Cilt: 18 Sayı: 2, 147 - 158, 01.06.2022
Mahmut Yanar
,
Kazım Ergin Gürkan
Ece Evliyaoğlu
Öz
Bu çalışmada, karanfil yağının ortalama ağırlığı 4,16±0,61 g olan japon balığının (Carassius auratus) derin anestezi (30 dk) ve taşınması (15 s) için etkili olan konsantrasyonları tespit edilmiştir. Ayrıca karanfil yağının balıkların 15 s süreli taşınmasında stoklama yoğunluğu üzerindeki etkisi belirlenmiştir. Karanfil yağının konsantrasyonu ve uygulama süresine bağlı olarak anesteziye giriş süresi 2 ile 11 dk, kendine gelme süresi ise 5 ile 17 dk arasında değişmiştir. Balıkların solunum sayısı anestezi içermeyen suda 73/dk iken, karanfil yağı uygulanmış olanlarda konsantrasyon ve uygulama süresine bağlı olarak 104/dk’ya kadar çıkmıştır. Karafil yağının 30 dk sürede hafif anestezi için 6-12 µL/L, derin anestezi için 18-24 µL/L; 15 s sürede hafif anestezi için 6-9 µL/L, derin anestezi için 12-18 µL/L konsantrasyonları güvenle kullanılabilir. Karanfil yağının toksik konsantrasyonları 30 dk uygulama için 42 µL/L, 15 s uygulama için 21 µL/L olarak saptanmıştır. Balıkların 15 s süreli taşınmasında taşıma suyuna karanfil yağı ilavesinin balığın yaşama oranı üzerine bir etkisi olmamıştır. Jüvenil japon balıklarının 3/4 oksijen basılmış naylon torbalarda 15 s süre ile taşınmasında, %98-99 yaşama oranı ile en uygun stoklama oranları 80 veya 100 adet/L’dir.
Destekleyen Kurum
Çukurova Üniversitesi Bilimsel Araştırmalar Projeleri Koordinasyon Birimi
Proje Numarası
FYL-2019-12032
Teşekkür
Bu çalışma yüksek lisans tezinden üretilmiştir. Bu tez çalışmasına FYL-2019-12032 nolu projeyle finansal destek sağlayan Çukurova Üniversitesi Bilimsel Araştırmalar Projeleri Koordinasyon Birimine teşekkür ederiz.
Kaynakça
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- Akbulut, B., Çakmak, E., Aksungur, N., & Çavdar,Y. (2011). Effect of exposure duration on time to recovery from anaesthesia of clove oil in juvenile of russian sturgeon. Turkish Journal of Fisheries and Aquatic Sciences, 11 (3), 463-467. https://doi.org/10.4194/1303-2712-v11_3_17
- Bennett, W.A., & Beitinger, T.L. (1997). Temperature tolerance of the sheepshead minnow, Cyprinodon variegatus. Copeia, (1), 77-87. https://doi.org/1447842
- Briozzo, J., Nuncez, L., Chirife, J., Herzage, L., & D’Aquino, M. (1989). Antimicrobial activity of clove oil dispersed in a concentrated sugar solution. Journal of Applied Bacteriology, 66, 69–75. https://doi.org/10.1111/j.1365-2672.1989.tb02456.x
- Bullerman, L. B., Lieu, F.Y., & Seier, S.A. (1977). Inhibition of growth and aflatoxin production by cinnamon and clove oils, cinnamic aldehyde and eugenol. Journal of Food Science, 42(4), 1107-1116. https://doi.org/10.1111/j.1365-2621.1977.tb12677.x
- Chellapan, A., Rajagopalsamy, C.B.T., & Jasmine, G.I. (2013). Effect of clove oil and benzocaine on the respiratory metabolism of angel fish Pterophyllum scalare. Indian Journal Science Technology, 6(7), 4853-4861.
- Cooke, S.J., Suski. C.D., Ostrand, K.G., Tufts, B.L., & Wahl, D.H. (2004). Behavioral and physiological assessment of low concentrations and transporting largemouth bass (Micropterus salmoides). Aquaculture, 239, 509-529. https://doi.org/10.1016/j.aquaculture.2004.06.028
- Dolezelova, P., Macova, S., Plhalova, L., Pistekova, V., & Svobodova, Z. (2011). The acute toxicity of clove oil to fish Danio rerio and Poecilia reticulata. Acta Veterinaria Brno, 80, 305–308. https://doi.org/10.2754/avb201180030305
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- Ghosh, A., Mahapatra, B.K., & Datta, N.C. (2003). Ornamental fish farming-successful small scale aqua business in India. Aquaculture Asia, 8 (3), 14-16.
- Grush,J., Noakes, D.L.G., & Moccia, R.D. (2004). The Efficacy of clove oil as an anesthetic for the zebrafish, Danio rerio (Hamilton). Zebrafish, 1 (1), 46- 53. https://doi.org/10.1089/154585404774101671
- Hajek, G.J., Klyszejko, B., & Dziaman, R.(2006). The anaesthetic effect of clove oil on common carp, Cyprinus carpio L. Acta Ichthyologica Piscatoria, 36(2), 93–97.
- Hamackova, J., Sedova J.M., Pjanova S.V., & Lepicova, A.(2002). The effect 2-phenoxyethanol, clove oil and propiscin anaesthetics on perch (Perca fluviatilis) in relation to water temperature. Czech Journal of Animal Science, 46 (11), 469–473.
- Han, M.C., Sağlıyan, A., & Polat, E. (2016). Akvaryum balıklarında karanfil yağının anestezik etkisinin araştırılması, Harran Üniversitesi Veteriner Fakültesi Dergisi, 5(1), 12-17.
- Hekimoğlu, M.A., & Ergun, M. (2012). Evaluation of clove oil as anaesthetic agent in fresh water angelfish, Pterophyllum scalare. Pakistan Journal Zoology, 44(5), 1297-1300.
- Hikasa, Y., Takase, K., Ogasawara, T., & Ogasawara, S.(1986). Anaesthesia and recovery with tricain methansulfonate, eugenol and thiopental sodium in carp, Cyprinus carpio. The Japanese Journal of Veterinary Science, 48 (2), 341-351. https://doi.org/10.1292/jvms1939.48.341
- Kaiser, H., Brill,G., Cahill, J., Collett, P., Czypionka, K., Green, A., Orr, K., Pattrick, P., Scheepers, R., Stonier, T., Whitehead, M.A., & Yearsley, R. (2006). Testing clove oil as an anaesthetic for long-distance transport of live fish: the case of the Lake Victoria cichlid Haplochromis obliquidens. Journal of Applied Ichthyology, 22, 510–514. https://doi.org/10.1111/j.1439-0426.2006.00786.x
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- Koçak, V., & Can, E. (2019). 2-Fenoksietanol ve karanfil esansiyel yağının sarı prenses (Labidochromis caeruleus) ve ahli (Sciaenochromis fryeri) balıkları üzerine anestetik etkileri. Biyoloji Bilimleri Araştırma Dergisi, 12 (2), 13-21.
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- Kramer, R.E. (1985). Antioxidants in clove. Jounal of American Oil Chemist Society, 62, 111-113. https://doi.org/10.1007/BF02541505
- Küçükosman, N. (2019). Karanfil (Eugenia caryophylatta), nane (Menta piperita) ve lavanta (Lavandula angustifolia) bitkilerinden elde edilen esansiyel yağların japon balıklarında (Carassius auratus, linnaeus, 1758) anestezik etkilerinin araştırılması ve uygun dozun belirlenmesi. Ordu üniversitesi Fen bilimleri enstitüsü, yüksek lisans tezi, Ordu.
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Optimal Concentration of Clove Oil in Anesthetization of Goldfish (Carassius auratus) and its Effect on Stocking Density for Fish Transportation
Yıl 2022,
Cilt: 18 Sayı: 2, 147 - 158, 01.06.2022
Mahmut Yanar
,
Kazım Ergin Gürkan
Ece Evliyaoğlu
Öz
In this study, the effective concentrations of clove oil for deep anesthesia (30 min) and transportation (15 h) of goldfish (Carassius auratus) with an average weight of 4.16±0.61g were determined. Besides, the effect of clove oil on stocking density in transporting fish for 15 hours was determined. Depending on the concentrations and application time of clove oil, induction time ranged from 2 to 11 min while recovery time varied between 5 and 17 min. Depending on the concentrations and application time of clove oil, the respiratory rate of fish increased from 73/min to 104/min with the addition of clove oil in the anesthetic-free water. 6-12 µL/L for light anesthesia and 18-24 µL/L for deep anesthesia at a 30 min period, 6-9 µL/L for light anesthesia and 12-18 µL/L for deep anesthesia at a 15 h period are determined concentrations of clove oil that can be used safely. The toxic concentrations of clove oil were determined as 42 µL/L for 30 min application, 21 µL/L for 15 h application. Adding clove oil to the transport water did not affect the survival rate of the fish during the 15 h transport period. When transporting juvenile goldfish in 3/4 oxygen compressed nylon bags for 15 h, the most suitable stocking rate is 80 or 100 fish/L with a 98-99% survival rate.
Proje Numarası
FYL-2019-12032
Kaynakça
- Abdolazizi, S., Ghaderi, E., Naghdi, N., & Kamangar, B. (2011). Effects of clove oil as an anesthetic on some hematological parameters of Carassius auratus. Aquaculture Research and Development, 2 (1), 1-3. http://dx.doi.org/10.4172/2155-9546.1000108
- Akbulut, B., Çakmak, E., Aksungur, N., & Çavdar,Y. (2011). Effect of exposure duration on time to recovery from anaesthesia of clove oil in juvenile of russian sturgeon. Turkish Journal of Fisheries and Aquatic Sciences, 11 (3), 463-467. https://doi.org/10.4194/1303-2712-v11_3_17
- Bennett, W.A., & Beitinger, T.L. (1997). Temperature tolerance of the sheepshead minnow, Cyprinodon variegatus. Copeia, (1), 77-87. https://doi.org/1447842
- Briozzo, J., Nuncez, L., Chirife, J., Herzage, L., & D’Aquino, M. (1989). Antimicrobial activity of clove oil dispersed in a concentrated sugar solution. Journal of Applied Bacteriology, 66, 69–75. https://doi.org/10.1111/j.1365-2672.1989.tb02456.x
- Bullerman, L. B., Lieu, F.Y., & Seier, S.A. (1977). Inhibition of growth and aflatoxin production by cinnamon and clove oils, cinnamic aldehyde and eugenol. Journal of Food Science, 42(4), 1107-1116. https://doi.org/10.1111/j.1365-2621.1977.tb12677.x
- Chellapan, A., Rajagopalsamy, C.B.T., & Jasmine, G.I. (2013). Effect of clove oil and benzocaine on the respiratory metabolism of angel fish Pterophyllum scalare. Indian Journal Science Technology, 6(7), 4853-4861.
- Cooke, S.J., Suski. C.D., Ostrand, K.G., Tufts, B.L., & Wahl, D.H. (2004). Behavioral and physiological assessment of low concentrations and transporting largemouth bass (Micropterus salmoides). Aquaculture, 239, 509-529. https://doi.org/10.1016/j.aquaculture.2004.06.028
- Dolezelova, P., Macova, S., Plhalova, L., Pistekova, V., & Svobodova, Z. (2011). The acute toxicity of clove oil to fish Danio rerio and Poecilia reticulata. Acta Veterinaria Brno, 80, 305–308. https://doi.org/10.2754/avb201180030305
- FAO, (2014). Ornamental fish. Food and agriculture organization of the United Nations. Fisheries and aquaculture departmant. www.fao.org/fishery/topic/13611/en (accessed 18 December 2014)
- Fischer, I.U., Unruh, G.E., & Dengler, H.J. (1990). The metabolism of eugenol in man. Xenobiotica, 20, 209–222. https://doi.org/10.3109/00498259009047156
- Ghosh, A., Mahapatra, B.K., & Datta, N.C. (2003). Ornamental fish farming-successful small scale aqua business in India. Aquaculture Asia, 8 (3), 14-16.
- Grush,J., Noakes, D.L.G., & Moccia, R.D. (2004). The Efficacy of clove oil as an anesthetic for the zebrafish, Danio rerio (Hamilton). Zebrafish, 1 (1), 46- 53. https://doi.org/10.1089/154585404774101671
- Hajek, G.J., Klyszejko, B., & Dziaman, R.(2006). The anaesthetic effect of clove oil on common carp, Cyprinus carpio L. Acta Ichthyologica Piscatoria, 36(2), 93–97.
- Hamackova, J., Sedova J.M., Pjanova S.V., & Lepicova, A.(2002). The effect 2-phenoxyethanol, clove oil and propiscin anaesthetics on perch (Perca fluviatilis) in relation to water temperature. Czech Journal of Animal Science, 46 (11), 469–473.
- Han, M.C., Sağlıyan, A., & Polat, E. (2016). Akvaryum balıklarında karanfil yağının anestezik etkisinin araştırılması, Harran Üniversitesi Veteriner Fakültesi Dergisi, 5(1), 12-17.
- Hekimoğlu, M.A., & Ergun, M. (2012). Evaluation of clove oil as anaesthetic agent in fresh water angelfish, Pterophyllum scalare. Pakistan Journal Zoology, 44(5), 1297-1300.
- Hikasa, Y., Takase, K., Ogasawara, T., & Ogasawara, S.(1986). Anaesthesia and recovery with tricain methansulfonate, eugenol and thiopental sodium in carp, Cyprinus carpio. The Japanese Journal of Veterinary Science, 48 (2), 341-351. https://doi.org/10.1292/jvms1939.48.341
- Kaiser, H., Brill,G., Cahill, J., Collett, P., Czypionka, K., Green, A., Orr, K., Pattrick, P., Scheepers, R., Stonier, T., Whitehead, M.A., & Yearsley, R. (2006). Testing clove oil as an anaesthetic for long-distance transport of live fish: the case of the Lake Victoria cichlid Haplochromis obliquidens. Journal of Applied Ichthyology, 22, 510–514. https://doi.org/10.1111/j.1439-0426.2006.00786.x
- Kamacı, H.O., Süzer, C., Çoban, D., Özdilek, G., Saka, Ş., & Fırat, K. (2009). Levrek (D. labrax) juvenillerinde tuzluluk değişimlerinin anestezi uygulamalarına olan etkileri: Karanfil yağı örneği. 15.Ulusal Su Ürünleri Sempozyumu, Rize.
- Keene, J.L., Noakes, D.L.G., Moccia, R.D., & Soto, C.G. (1998). The efficacy of clove oil as an anaesthetic for rainbow trout, Onchorhynchus mykiss (Walbaum). Aquaculture Research, 29, 89-101. https://doi.org/10.1046/j.1365-2109.1998.00927.x
- Koçak, V., & Can, E. (2019). 2-Fenoksietanol ve karanfil esansiyel yağının sarı prenses (Labidochromis caeruleus) ve ahli (Sciaenochromis fryeri) balıkları üzerine anestetik etkileri. Biyoloji Bilimleri Araştırma Dergisi, 12 (2), 13-21.
- Kolarova, J., Svobodova, Z., Nepejchalova, L., Velisek, J., & Piackova, V. (2006). Anaesthesia of fish in Czech Republic. Bulletin VURH Vodnany, 42, 105-108.
- Kramer, R.E. (1985). Antioxidants in clove. Jounal of American Oil Chemist Society, 62, 111-113. https://doi.org/10.1007/BF02541505
- Küçükosman, N. (2019). Karanfil (Eugenia caryophylatta), nane (Menta piperita) ve lavanta (Lavandula angustifolia) bitkilerinden elde edilen esansiyel yağların japon balıklarında (Carassius auratus, linnaeus, 1758) anestezik etkilerinin araştırılması ve uygun dozun belirlenmesi. Ordu üniversitesi Fen bilimleri enstitüsü, yüksek lisans tezi, Ordu.
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