Bozcaada çavuş ve karalahna üzümlerinden izole edilen okratoksijenik Aspergillus türlerinin biyoçeşitliliği

Öz

Bu çalışmada, Bozcaada Çavuş ve Karalahna üzümlerinden izole edilen okratoksijenik Aspergillus türlerinin varlığı ve OTA üretimi rapor edilmiştir. Çalışma, 2015 ve 2016 yıllarında olgunlaşma sırasında toplanan üzüm örneklerinden izole edilen Aspergillus izolatları ile gerçekleştirilmiştir. İlk olarak 290 Aspergillus izolatının ikincil metabolit profilleri İnce Tabaka Kromatografisi kullanılarak belirlenmiştir. İzolatlardan 122 tanesinin ise muhtemel OTA üreticisi olduğu tespit edilmiştir. 122 izolatın farklı kültür ortamlarındaki koloni morfolojilerine göre seçilen 43 izolat, calmodulin gen dizileme analizi kullanılarak tanımlanmıştır. Tanımlanan izolatların A. tubingensis, A. carbonarius, A. niger/welwitschia/awamori, A. welwitschia, A. spelaeus ve A. fructus olduğu belirlenmiştir. OTA üretimi ise altı izolatta araştırılmıştır. HPLC-FLD kullanılarak bu izolatların 3,550 ± 0,240 ile 92,346 ± 0,818 ppb arasında değişen miktarlarda OTA ürettiği bulunmuştur. Sonuç olarak üzümlerden OTA üreten Aspergillus türleri izole edildi. A. spelaeus ve A. fructus türlerinin varlığı üzümlerde ilk kez rapor edilmiştir. A. fructus Türkiye için yenit kayıt olduğu tespit edilmiştir.

Anahtar Kelimeler

• Aspergillus
• calmodulin
• HPLC
• Moleküler identifikasyon
• TLC.

Proje Numarası

FBA-2019-3028

Biodiversity of ochratoxigenic Aspergillus species isolated from çavuş and karalahna grapes in Bozcaada, Türkiye

Öz

This study reported the presence and OTA production of ochratoxigenic Aspergillus species isolated from Bozcaada Çavuş and Karalahna grapes. The study was conducted with Aspergillus isolates isolated from grape samples collected during ripening in 2015 and 2016. Thin-layer chromatography was used to determine the secondary metabolite profiles of 290 Aspergillus isolates. Out of these, 122 isolates were found to be possible OTA producers. 43 isolates, selected based on their colony morphology of 122 isolates in different culture media, were identified using calmodulin gene sequencing analysis. The identified isolates were determined to be A. tubingensis, A. carbonarius, A. niger/welwitschia/awamori, A. welwitschia, A. spelaeus, and A. fructus. OTA production was investigated in six isolates. Using HPLC-FLD, these isolates were found to produce OTA in quantities ranging from 3.550 ± 0.240 to 92.346 ± 0.818 ppb. Consequently, OTA-producing Aspergillus species were isolated from grapes. The presence of A. spelaeus and A. fructus was reported for the first time in grapes. A. fructus has been found to be a new record for Türkiye.

Anahtar Kelimeler

• Aspergillus
• Calmodulin
• HPLC
• Molecular Identification
• TLC.

Destekleyen Kurum

This study was supported by Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit with project number FBA-2019-3028.

Proje Numarası

FBA-2019-3028

Teşekkür

We would like to thank Erhan AKDAĞ, Deputy Director of the Çanakkale Food Control Laboratory Directorate of the Ministry of Agriculture and Forestry, and the staff of the Mycotoxin Laboratory Unit, who helped with the ochratoxin analysis within the scope of the study. We thank Assoc. Prof. Dr. Alper Tunga AKARSUBAŞI for her support for the article.

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