Improvement of Crude Hazelnut Oil Yield and Quality by Aqueous Enzymatic Extraction

Yıl 2018, , 301 - 306, 29.10.2018

Özge Ermiş Caner Kazma Duygu Kıbıcı Derya Kahveci

https://doi.org/10.24323/akademik-gida.475359

Öz

In the last decade, search for healthier methods that can minimize the loss of oil quality has increased instead of a solvent extraction method, which is commonly used in the production of edible oils in food industry. Aqueous enzymatic extraction is a potential alternative to solvent extraction. In this study, hazelnut oil was extracted from raw hazelnuts at 50°C by using 2% Pectinex®, 2% Viscozyme® or their equal mixture (2% based on hazelnut weight). The oil yield and the quality characteristics of extracted oils were compared with oils obtained by solvent extraction (chloroform:methanol). For this purpose, free acidity and peroxide values of extracted oils were determined. Results indicated that methods used for crude oil extraction had a significant effect on crude oil yield and the free acidity and peroxide values of extracted oils (p<0.05). The highest yield (70.74±2.68%) was obtained in the extraction containing equal mixtures of Pectinex® and Viscozyme®. The lowest free acidity and peroxide values were determined in oils obtained by Viscozyme® enzyme alone.

Anahtar Kelimeler

Oil extraction, Aqueous enzymatic extraction, Cellulase, Pectinase

Sulu Enzimatik Ekstraksiyon ile Fındık Yağında Verim ve Kalitenin Geliştirilmesi

Öz

Son on yılda, endüstriyel yemeklik yağ
üretiminde yaygın olarak kullanılan çözgen ekstraksiyon yöntemi yerine daha sağlıklı
ve yağda kalite kaybını en aza indirebilecek yöntemlerin arayışı hız
kazanmıştır. Sulu enzimatik ekstraksiyon, çözgen ekstraksiyon yöntemine
alternatif olarak öne çıkan yöntemlerdendir. Bu çalışmada, ham fındıktan 50°C’de
fındığın kütlesine göre %2 Pectinex® ya da %2 Viscozyme® enzimleri ile bu iki
enzimin eşit oranda karışımı eklenerek ham yağ elde edilmiştir. Sulu enzimatik
ekstraksiyon yönteminin yağ verimi ve elde edilen yağın kalite özellikleri çözgen
ektraksiyon (kloroform:metanol) yöntemi kullanılarak elde edilen yağ ile
karşılaştırılmıştır. Bu amaçla yağların serbest asitlik ve peroksit değeri
belirlenmiştir. Ham yağ ekstraksiyonu için kullanılan farklı yöntemlerin ham
yağ verimi ile elde edilen yağların serbest asitlik ve peroksit değerleri üzerinde
önemli bir etkisi olduğu gözlemlenmiştir (p<0.05).
Bu denemeler sonucunda en yüksek verim %70.74±2.68 ile Pectinex® ve Viscozyme®
enzimlerinin ikili karışımı ile yapılan ekstraksiyonda sağlanmıştır. En düşük
serbest asitlik ve peroksit sayısı değerleri ise Viscozyme® enzimi tek başına
kullanılarak elde edilen yağlarda belirlenmiştir.

Anahtar Kelimeler

Yağ ekstraksiyonu, Sulu enzimatik ekstraksiyon, Selülaz, Pektinaz

Kaynakça

• [1] Anonim, (2015). Fındık sektör raporu, 2014, Toprak Mahsulleri Ofisi Genel Müdürlüğü, Ankara.
• [2] Yavuz G.G., Polat, K. (2012). Durum ve Tahmin Fındık 2011/2012, TEPGE Yayın No: 1918 ISBN: 978-975-407-338-6 ISSN: 1306-0260, T.C. Gıda, Tarım ve Hayvancılık Bakanlığı Tarımsal Ekonomi ve Politika Geliştirme Enstitüsü, Ankara.
• [3] Taş, N.G., Gökmen V. (2015). Profiling triacylglycerols, fatty acids and tocopherols in hazelnut varieties grown in Turkey, Journal of Food Composition and Analysis, 44, 115-121.
• [4] Tunç, İ., Çalışkan, F., Özkan, G., Karacabey, E. (2014). Mikrodalga destekli Soxhlet cihazı ile fındık yağı ekstraksiyonunun yanıt yüzey yöntemi ile optimizasyonu. Akademik Gıda Dergisi, 12(1), 20-28.
• [5] Fındık yağı (Türkomp Gıda Kodu: 05.02.0004), Sıvı ve Katı Yağlar, Ulusal Gıda Kompozisyon Veri Tabanı verisi (Son erişim: 15.11.2016).
• [6] Kavrulmuş Fındık, İç (Türkomp Gıda Kodu: 09.02.0057), Meyve ve Meyve Türleri, Ulusal Gıda Kompozisyon Veri Tabanı Verisi (Son erişim: 15.11.2016).
• [7] Kwaku, T., Ohta, Y. (1997). Aqueous extraction of coconut oil by an enzyme-assisted. Journal of the Science of Food and Agriculture, 74, 497-502.
• [8] Holum, J. (1968). Elements of General and Biological Chemistry, 2nd edition, Wiley, New York, 377p.
• [9] Rosenthal, A., Pyle, D.L., Niranjan, K. (1996). Aqueous and enzymatic processes for edible oil extraction. Enzyme and Microbial Technology, 19(6), 402-420.
• [10] Singh, R.K., Sarker, B.C., Kumbhar, B.K., Agrawal, Y.C., Kulshreshtha, M.K. (1999). Response surface analysis of enzyme assisted oil extraction factors for sesame, groundnut and sunflower seeds. Journal of Food Science and Technology, 36(6), 511-514.
• [11] Bligh, E.G., Dyer, W.J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911-917.
• [12] Leitgeb, M., Knez, Ž. (1990). The influence of water on the synthesis of n-butyl oleate by immobilized Mucor miehei lipase. Journal of the American Oil Chemists’ Society, 67(11), 775-778.
• [13] Shantha, N.C., Decker, E.A. (1994). Rapid, sensitive, iron-based spectrophotometric methods for determination of peroxide values of food lipids. Journal of AOAC International, 77(2), 421-424.
• [14] Concha, J., Soto, C., Chamy, R., Zuniga, M.E. (2004). Enzymatic pretreatment on rose-hip oil extraction: hydrolysis and pressing conditions. Journal of the American Oil Chemists’ Society, 81(6), 549-552.
• [15] Latif, S., Anwar, F. (2011). Aqueous enzymatic sesame oil and protein extraction. Food Chemistry, 125, 679-684.
• [16] Campbell, K.A., Vaca-Medina, G., Glatz, C.E., Pontalier, P.Y. (2016). Parameters affecting enzyme-assisted aqueous extraction of extruded sunflower meal. Food Chemistry, 208, 245-251.
• [17] Shah, S., Sharma, A., Gupta, M.N. (2005). Extraction of oil from Jatropha curcas L. seed kernels by combination of ultrasonication and aqueous enzymatic oil extraction. Bioresource Technology, 96(1), 121-123.
• [18] Anonim, (2017). Polarity Index, Louisiana State University, USA. http://macro.lsu.edu/HowTo/solvents/Polarity%20index.htm (Erişim Tarihi: 01.11.2016).
• [19] E.Choek., Min D.B. (2009). Mechanisms of Antioxidants in the Oxidation of the Food, Comprehensive Reviews in Food Science and Food Safety, 345, 358.
• [20] Fang, X., Fei, X., Sun, H., Jin, Y. (2016). Aqueous enzymatic extraction and demulsification of camellia seed oil (Camellia oleifera Abel.) and the oil’s physicochemical properties. European Journal of lipid Science and Technology, 118, 244-251.
• [21] Chen, F., Zhang, Q., Gu, H., Yang, L. (2016). An approach for extraction of kernel oil from Pinus pumila using homogenate-circulating ultrasound in combination with an aqueous enzymatic process and evaluation of its antioxidant activity. Journal of Chromatography A, 1471, 68-79.
• [22] Mehanni, A.E.S., El-Reffaei, W.H.M., Melo, A., Casal, S., Ferreira, I.M.P.L.V.O. (2017). Enzymatıc extraction of oil from Balanites aegyptiaca (desert date) kernel and comparison with solvent extracted oil. Journal of Food Biochemistry, 41, e12270-e12275.
• [23] Tan, Z.J., Yang, Z.Z., Yi, Y.J., Wang, H.Y., Zhou, W.L., Li, F.F., Wang, C.Y. (2016). Extraction of oil from flaxseed (Linum usitatissimum L.) using enzyme-assisted three-phase partitioning. Applied Biochemistry and Biotechnology, 179, 1325-1335.
• [24] Latif, S., Diosady, L.L., Anwar, F. (2008). Enzyme-assisted aqueous extraction of oil and protein from canola (Brassica napus L.) seeds. European Journal of Lipid Science and Technology, 110(10), 887-892.
• [25] Li, X.J., Li, Z.G., Wang,X., Han, J.Y., Zhang, B., Fu, Y. J., Zhao, C. J. (2016). Application of cavitation system to accelerate aqueous enzymatic extraction of seed oil from Cucurbita pepo L. and evaluation of hypoglycemic effect. Food Chemistry, 212, 403-410.
• [26] Ezeh, O., Niranjan, K., Gordon, M.H. (2016). Effect of enzyme pre‑treatments on bioactive compounds in extracted tiger nut oil and sugars in residual meals. Journal of the American Oil Chemists' Society, 93, 1541-1549.
• [27] Ozdemir, M., Ackurt, F., Kaplan, M., Yildiz, M., Loker, M., Gurcan, T., Seyhan, F.G. (2001). Evaluation of new Turkish hybrid hazelnut (Corylus avellana L.) varieties: fatty acid composition, α-tocopherol content, mineral composition and stability. Food Chemistry, 73(4), 411-415.