Yıl 2019, Cilt 17 , Sayı 4, Sayfalar 444 - 449 2019-12-31

Yeşil Salatalardan Pseudomonas fluorescens İzolasyonu ve İzolatların Antibiyotik Duyarlılıkları
Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates

Dilek Düyüncü [1] , Seyhan Ulusoy [2]

Günümüzde yeşil salatalar genellikle pratik ve sağlıklı olduklarını düşünerek tüketilmektedir. Ancak salataların hazırlanması sırasında, salata malzemelerinin yetersiz yıkanması ve hijyenik olmayan yüzeylerle teması, salataların mikrobiyal yükünü arttırmaktadır. Bu durum salataları tüketen bireyler için sağlık sorunlarına neden olabilir. Antibiyotiklerin aşırı kullanımı, gıda kaynaklı patojenler dahil olmak üzere çoklu ilaç direncine sahip bakterilerin ortaya çıkmasına neden olmuştur. Bu patojen bakterilerin biyofilm üretme özelliklerinin olması, bu patojenlerin sebep oldukları enfeksiyonların tedavisini zorlaştırmaktadır. Bu çalışmanın amacı, farklı restoranlardan temin edilen yeşil salatalardan P. fluorescens izolasyonu ve tanımlanmasıdır. Yeşil salatalardan toplam 72 izolat izole edilmiş ve bu izolatların 29'u PZR ile Pseudomonas olarak ve 9'u P. fluorescens olarak tanımlanmıştır. Tüm P. fluorescens izolatlarının ampisilin, amoksisilin, sefuroksim, seftazidime ve seftriakson antibiyotiklerine dirençli olduğu bulunmuştur. Bu çalışmanın sonuçları, yeşil salataların hazırlanması ve depolanması sırasında hijyen koşullarına daha fazla dikkat edilmesi gerektiğini göstermektedir.
Currently, leafy green salads are often consumed because they are considered as practical and healthy. However, during their preparation, both inadequate washing and contact with non-hygienic surfaces may increase their microbial load. This may cause several health problems for individuals consuming salads. The overuse of antibiotics has led to the emergence of multidrug resistant bacteria, including foodborne pathogens. Biofilm production ability of these pathogenic bacteria makes it difficult to treat infections caused by these pathogens. The aim of this study is to isolate and identify P. fluorescens from leafy green salads collected from different restaurants. A total of 72 isolates were isolated from leafy green salads, and 29 of these isolates were identified by PCR as Pseudomonas and 9 of them identified as P. fluorescens. All P. fluorescens isolates were resistant to ampicillin, amoxicillin, cefuroxime, ceftazidime and ceftriaxone antibiotics. The results of this study showed that additional attention for the hygiene conditions is needed during the preparation and storage stages of leafy green salads.
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Birincil Dil en
Konular Gıda Bilimi ve Teknolojisi
Bölüm Araştırma Makaleleri

Orcid: 0000-0001-7739-9230
Yazar: Dilek Düyüncü
Ülke: Turkey

Orcid: 0000-0002-6559-1177
Yazar: Seyhan Ulusoy (Sorumlu Yazar)
Ülke: Turkey

Destekleyen Kurum Süleyman Demirel University Scientific Research Projects Management Unit
Proje Numarası 3955-YL1-14
Teşekkür We would like to thank the Head of Scientific Research Projects Management Unit of Süleyman Demirel University for supporting this study with the project number 3955-YL1-14.

Yayımlanma Tarihi : 31 Aralık 2019

Bibtex @araştırma makalesi { akademik-gida667250, journal = {Akademik Gıda}, issn = {1304-7582}, eissn = {2148-015X}, address = {Fevzipaşa Bulv. Çelik İş Merkezi, No: 162, Kat: 3, D:302, Çankaya, İzmir}, publisher = {Sidas Medya Ajans Tanıtım Danışmanlık Ltd. Şti.}, year = {2019}, volume = {17}, pages = {444 - 449}, doi = {10.24323/akademik-gida.667250}, title = {Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates}, key = {cite}, author = {Düyüncü, Dilek and Ulusoy, Seyhan} }
APA Düyüncü, D , Ulusoy, S . (2019). Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates. Akademik Gıda , 17 (4) , 444-449 . DOI: 10.24323/akademik-gida.667250
MLA Düyüncü, D , Ulusoy, S . "Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates". Akademik Gıda 17 (2019 ): 444-449 <https://dergipark.org.tr/tr/pub/akademik-gida/issue/51394/667250>
Chicago Düyüncü, D , Ulusoy, S . "Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates". Akademik Gıda 17 (2019 ): 444-449
RIS TY - JOUR T1 - Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates AU - Dilek Düyüncü , Seyhan Ulusoy Y1 - 2019 PY - 2019 N1 - doi: 10.24323/akademik-gida.667250 DO - 10.24323/akademik-gida.667250 T2 - Akademik Gıda JF - Journal JO - JOR SP - 444 EP - 449 VL - 17 IS - 4 SN - 1304-7582-2148-015X M3 - doi: 10.24323/akademik-gida.667250 UR - https://doi.org/10.24323/akademik-gida.667250 Y2 - 2019 ER -
EndNote %0 Akademik Gıda Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates %A Dilek Düyüncü , Seyhan Ulusoy %T Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates %D 2019 %J Akademik Gıda %P 1304-7582-2148-015X %V 17 %N 4 %R doi: 10.24323/akademik-gida.667250 %U 10.24323/akademik-gida.667250
ISNAD Düyüncü, Dilek , Ulusoy, Seyhan . "Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates". Akademik Gıda 17 / 4 (Aralık 2020): 444-449 . https://doi.org/10.24323/akademik-gida.667250
AMA Düyüncü D , Ulusoy S . Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates. Akademik Gıda. 2019; 17(4): 444-449.
Vancouver Düyüncü D , Ulusoy S . Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates. Akademik Gıda. 2019; 17(4): 449-444.