Propolis Püskürtülmüş Ambalaj Filmlerin Antibakteriyel Aktiviteleri
Yıl 2021,
Cilt: 19 Sayı: 1, 21 - 27, 26.04.2021
Gizem Bayatbalay
Ezgi Karpuz
,
İbrahim Palabıyık
Öz
Yapılan bu çalışmada, farklı çözgenler kullanılarak oluşturulan propolis ekstraktlarının, plastik yüzeye tutunma özellikleri ve antibakteriyel aktivitesi belirlenmiştir. Çözgen olarak vaks-yağ, yağ, gliserol, film solüsyonu, etanol, etil asetat ve propilen glikol kullanılmıştır. Belli konsantrasyonlarda hazırlanmış olan propolis ve antibakteriyel solüsyonlar püskürtme metodu uygulanarak düşük yoğunluklu polietilen (LDPE) filmlere entegre edilmiştir. Yağ içeren solüsyonların film yüzeyinde tutunma göstermediği tespit edilmiştir. Propolisli solüsyonlar farklı antibakteriyel solüsyonlar ile kıyaslanarak, istenilen kriterlerde LDPE filme entegre edilebilecek en uygun sıvının etil asetatlı propolis solüsyonu olduğu tespit edilmiştir. Bu solüsyon püskürtülerek elde edilen propolisli LDPE filmlerde ISO 22196:2011 metodu uygulanarak, filmlerin antibakteriyel etkileri tespit edilmiş ve söz konusu bakterileri 24. saat itibari ile 7 kob/g azalttığı görülmüştür.
Destekleyen Kurum
Tekirdağ Namık Kemal Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi
Proje Numarası
NKUBAP.03.YL.19.225
Teşekkür
Bu çalışma Tekirdağ Namık Kemal Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimince NKUBAP.03.YL.19.225 Proje Numarası ile desteklenmiştir.
Kaynakça
- [1] Castolda, S., Capasso, F. (2009). Propolis an old remedy used in modern medicine. Fitoterapia, 73, 51-56.
- [2] Kutluca, S., Genç, F., Korkmaz, A. (2006). Propolis. Samsun Tarım İl Müdürlüğü Çiftçi Eğitimi ve Yayım Şubesi, Samsun, p. 57.
- [3] Şahinler, N. (2000). Arı ürünleri ve insan sağlığı açısından önemi. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 5(1-2), 139-148.
- [4] Hepşen, İ.F., Tilgen, F., Hamdi E. (1996). Propolis: Tıbbi özellikleri ve oftalmolojik kullanımı. Turgut Özal Tıp Merkezi Dergisi, 3(4), 386-391.
- [5] Markham, K.R., Mitchell, K.A., Wilkins, A.L., Daldy, J.A., Lu, Y. (1996). HPLC and GC-MS identification of the major organic constituents in New Zealand propolis. Phytochemistry, 42, 205-211.
- [6] Kartal, M., Kaya, S., Kurucu, S. (2002). GC-MS analysis of propolis samples from two different regions of Turkey. Zeitschrift für Naturforsch, 57, 905-909.
- [7] Burdock, G.A. (1998). Review of the biological properties and toxicity of bee propolis. Food and Chemical Toxicology, 36, 347-363.
- [8] Takaisi-Kikuni, N.B., Schilcher, H. (1994). Electron microscopic and microcalorimetric investigations of the possible mechanism of the antibacterial action of a defined propolis provenance. Planta Medica, 60, 222-227.
- [9] Üçüncü, M. (2007). Food Packing Technology (in Turkish), Ege Üniversitesi Mühendislik Fakültesi Gıda Mühendisliği Bölümü, İzmir, 896s.
- [10] Torlak, E., Sert, D. (2013). Antibacterial effectiveness of chitosan–propolis coated polypropylene films against foodborne pathogens. International Journal of Biological Macromolecules, 60, 52-55.
- [11] Han, J.H., and J.D. Floros. (1997). Casting antimicrobial packaging films and measuring their physical properties and antimicrobial activity. Journal of Plastic Film and Sheeting, 13(4), 287-298.
- [12] Marcucci, M.C. (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26, 83-89.
- [13] Nieva, M.M.I., Isla, M.I., Cudmani, N.G., Vattuone, M.A., Sampietro, A.R. (1999). Screening of antibacterial activity of Amaicha del Valle (Tucuman, Argentina) propolis. Journal of Ethnopharmacol, 68, 97-102.
- [14] Sforcin, J.M., Fernandes, Jr., A., Lopes, C.A.M., Bankova, V., Funari, S.R.C. (2000). Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacol, 73, 243-249.
- [15] Grange, J.M., Davey, R.W. (1990). Antibacterial properties of propolis (bee glue). Journal of the Royal Society of Medicine, 83, 159-161.
- [16] Dobrowolski, J.W., Vohora, S.B., Sharma, K., Shah, S.A., Naqvi, S.A.H., Dandiya, P.C. (1991). Antibacterial, antifungal, antiamoebic, antiinflammatory and antipyretic studies on propolis bee products. Journal of Etnopharmacol, 35, 77-82.
- [17] Mirzoeva, O. K., Grishanin, R. N., Calder, P. C. (1997). Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiological Research, 152, 239-246.
- [18] Uğur, A., Arslan, T. (2004). An in vitro study on antimicrobial activity of propolis from Mugla province of Turkey. Journal of Medicinal Food, 7 (1), 90-94.
- [19] Silici, S., Kutluca, S. (2005). Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. Journal of Ethnopharmacol, 99, 69-73.
- [20] Dığrak M, Yılmaz Ö, Çelik S, Yıldız S. (1995). Propolisteki yağ asitleri ve antimikrobiyal etkisi üzerinde in vitro araştırmalar. Gıda, 20(4), 249-255.
- [21] Noureddine, H., Hage-Sleiman, R., Wehbi, B., Fayyad-Kazan, A.H., Hayar, S., Traboulssi, M., ElMakhour, Y. (2017). Chemical characterization and cytotoxic activity evaluation of Lebanese propolis. Biomedicine & Pharmacotherapy, 95, 298-307.
- [22] Silva, C., Prasniewski, A., Calegari, M.A., de Lima, V.A., Oldoni, T.L. (2018). Determination of total phenolic compounds and antioxidant activity of ethanolic extracts of propolis using ATR–FT-IR. Food Analytical Methods, 11, 2013–2021.
- [23] Cottica, S.M., Sabik, H., Antoine, C., Fortin, J., Graveline, N., Visentainer, J.V., Britten, M. (2015). Characterization of Canadian propolis fractions obtained from two-step sequential extraction. Food Science and Technology, 60(1), 609-614.
- [24] Ertürk, Ö., Yavuz, C., Sıralı, R. (2014). The antimicrobial activity of propolis from Ordu province of Turkey. Mellifera, 14(27-28), 11-16.
- [25] Segueni, N., Khadraoui, F., Rhouati, S. (2017). Volatile compounds as propolis characterization markers. In Euro-Mediterranean Conference for Environmental Integration. Springer, 16(4), 1271-1273.
- [26] Bakdash, A., Almohammadi, O.H., Taha, N.A., Abu-Rumman, A., Kumar, S. (2018). Chemical composition of propolis from the Baha Region in Saudi Arabia. Czech Journal of Food Science, 36(2), 00-10.
- [27] Ghamdi, A.A., Bayaqoob, N.I., Rushdi, A.I., Alattal, Y., Simoneit, B.R., El-Mubarak, A.H., Al-Mutlaq, K.F. (2017). Chemical compositions and characteristics of organic compounds in propolis from Yemen. Saudi Journal of Biological Sciences, 24(5), 1094-1103.
- [28] Mohtar, L.G., Rodríguez, S.A., Nazareno, M.A. (2017). Comparative analysis of volatile compound profiles of propolis from different provenances. Journal of the Science of Food and Agriculture, 98(9), 3409-3415.
- [29] Taddeo, V.A., Epifano, F., Fiorito, S., Genovese, S. (2016). Comparison of different extraction methods and HPLC quantification of prenylated and unprenylated phenylpropanoids in raw Italian propolis. Journal of Pharmaceutical and Biomedical Analysis, 129, 219-223.
- [30] Netíková, L., Bogusch, P., Heneberg, P. (2013). Czech ethanol‐free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens. Journal of Food Science, 78(9), 1421-1429.
- [31] Arslan, S., Perçin, D., Silici, S., Er, Ö. (2010). Farklı çözücülerle hazırlanan propolis özütlerinin mutans streptokoklar üzerine in vitro antimikrobiyal etkisi. Sağlık Bilimleri Dergisi, 19(1), 68.
- [32] ISO 22196:2011, Plastics-Measurement of Antibacterial Activity on Plastics and Other Non-porous Surfaces. Geneva, Switzerland.
- [33] Bakkaloğlu, Z., Arıcı M. (2019). Farklı çözücülerle propolis ekstraksiyonunun toplam fenolik içeriği, antioksidan kapasite ve antimikrobiyal aktivite üzerine etkileri. Akademik Gıda, 17(4) 538-545.
- [34] Mascheroni, E., Guillard, V., Nalin, F., Mora, L., Piergiovanni, L. (2010). Diffusivity of propolis compounds in Polylactic acid polymer for the development of anti-microbial packaging films. Journal of Food Engineering, 98, 294-301.
- [35] Martí, M., Frígols, B., Serrano-Aroca, A. (2018). Antimicrobial characterization of advanced materials for bioengineering applications. The Journal of Visualized Experiments, 28(4), 138.
- [36] Japanese Standards Association (2000). Japanese Industrial Standard JIS Z 2801:2000. Antimicrobial products-test for antimicrobial activity and efficacy. Tokyo, Japan.
Antibacterial Activities of Propolis-Sprayed Packaging Films
Yıl 2021,
Cilt: 19 Sayı: 1, 21 - 27, 26.04.2021
Gizem Bayatbalay
Ezgi Karpuz
,
İbrahim Palabıyık
Öz
In this study, the adhesion properties and antibacterial activity of propolis extracts produced by using different solvents were determined. Wax-oil, oil, glycerol, film solution, ethanol, ethyl acetate and propylene glycol were used as solvents. Propolis and antibacterial solutions prepared at certain concentrations were integrated into low density polyethylene (LDPE) films by a spraying method. It was determined that the oil containing solutions did not adhere on a film surface. By comparing propolis solutions with different antibacterial solutions, it was found that the most suitable liquid that can be integrated onto the LDPE film in desired criteria was propolis solution with ethyl acetate. The antibacterial effects of films were determined by the ISO 22196: 2011 method on propolis-sprayed LDPE films, and it was observed that bacterial count was reduced by 7 cfu/g after 24 hour.
Proje Numarası
NKUBAP.03.YL.19.225
Kaynakça
- [1] Castolda, S., Capasso, F. (2009). Propolis an old remedy used in modern medicine. Fitoterapia, 73, 51-56.
- [2] Kutluca, S., Genç, F., Korkmaz, A. (2006). Propolis. Samsun Tarım İl Müdürlüğü Çiftçi Eğitimi ve Yayım Şubesi, Samsun, p. 57.
- [3] Şahinler, N. (2000). Arı ürünleri ve insan sağlığı açısından önemi. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 5(1-2), 139-148.
- [4] Hepşen, İ.F., Tilgen, F., Hamdi E. (1996). Propolis: Tıbbi özellikleri ve oftalmolojik kullanımı. Turgut Özal Tıp Merkezi Dergisi, 3(4), 386-391.
- [5] Markham, K.R., Mitchell, K.A., Wilkins, A.L., Daldy, J.A., Lu, Y. (1996). HPLC and GC-MS identification of the major organic constituents in New Zealand propolis. Phytochemistry, 42, 205-211.
- [6] Kartal, M., Kaya, S., Kurucu, S. (2002). GC-MS analysis of propolis samples from two different regions of Turkey. Zeitschrift für Naturforsch, 57, 905-909.
- [7] Burdock, G.A. (1998). Review of the biological properties and toxicity of bee propolis. Food and Chemical Toxicology, 36, 347-363.
- [8] Takaisi-Kikuni, N.B., Schilcher, H. (1994). Electron microscopic and microcalorimetric investigations of the possible mechanism of the antibacterial action of a defined propolis provenance. Planta Medica, 60, 222-227.
- [9] Üçüncü, M. (2007). Food Packing Technology (in Turkish), Ege Üniversitesi Mühendislik Fakültesi Gıda Mühendisliği Bölümü, İzmir, 896s.
- [10] Torlak, E., Sert, D. (2013). Antibacterial effectiveness of chitosan–propolis coated polypropylene films against foodborne pathogens. International Journal of Biological Macromolecules, 60, 52-55.
- [11] Han, J.H., and J.D. Floros. (1997). Casting antimicrobial packaging films and measuring their physical properties and antimicrobial activity. Journal of Plastic Film and Sheeting, 13(4), 287-298.
- [12] Marcucci, M.C. (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26, 83-89.
- [13] Nieva, M.M.I., Isla, M.I., Cudmani, N.G., Vattuone, M.A., Sampietro, A.R. (1999). Screening of antibacterial activity of Amaicha del Valle (Tucuman, Argentina) propolis. Journal of Ethnopharmacol, 68, 97-102.
- [14] Sforcin, J.M., Fernandes, Jr., A., Lopes, C.A.M., Bankova, V., Funari, S.R.C. (2000). Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacol, 73, 243-249.
- [15] Grange, J.M., Davey, R.W. (1990). Antibacterial properties of propolis (bee glue). Journal of the Royal Society of Medicine, 83, 159-161.
- [16] Dobrowolski, J.W., Vohora, S.B., Sharma, K., Shah, S.A., Naqvi, S.A.H., Dandiya, P.C. (1991). Antibacterial, antifungal, antiamoebic, antiinflammatory and antipyretic studies on propolis bee products. Journal of Etnopharmacol, 35, 77-82.
- [17] Mirzoeva, O. K., Grishanin, R. N., Calder, P. C. (1997). Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiological Research, 152, 239-246.
- [18] Uğur, A., Arslan, T. (2004). An in vitro study on antimicrobial activity of propolis from Mugla province of Turkey. Journal of Medicinal Food, 7 (1), 90-94.
- [19] Silici, S., Kutluca, S. (2005). Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. Journal of Ethnopharmacol, 99, 69-73.
- [20] Dığrak M, Yılmaz Ö, Çelik S, Yıldız S. (1995). Propolisteki yağ asitleri ve antimikrobiyal etkisi üzerinde in vitro araştırmalar. Gıda, 20(4), 249-255.
- [21] Noureddine, H., Hage-Sleiman, R., Wehbi, B., Fayyad-Kazan, A.H., Hayar, S., Traboulssi, M., ElMakhour, Y. (2017). Chemical characterization and cytotoxic activity evaluation of Lebanese propolis. Biomedicine & Pharmacotherapy, 95, 298-307.
- [22] Silva, C., Prasniewski, A., Calegari, M.A., de Lima, V.A., Oldoni, T.L. (2018). Determination of total phenolic compounds and antioxidant activity of ethanolic extracts of propolis using ATR–FT-IR. Food Analytical Methods, 11, 2013–2021.
- [23] Cottica, S.M., Sabik, H., Antoine, C., Fortin, J., Graveline, N., Visentainer, J.V., Britten, M. (2015). Characterization of Canadian propolis fractions obtained from two-step sequential extraction. Food Science and Technology, 60(1), 609-614.
- [24] Ertürk, Ö., Yavuz, C., Sıralı, R. (2014). The antimicrobial activity of propolis from Ordu province of Turkey. Mellifera, 14(27-28), 11-16.
- [25] Segueni, N., Khadraoui, F., Rhouati, S. (2017). Volatile compounds as propolis characterization markers. In Euro-Mediterranean Conference for Environmental Integration. Springer, 16(4), 1271-1273.
- [26] Bakdash, A., Almohammadi, O.H., Taha, N.A., Abu-Rumman, A., Kumar, S. (2018). Chemical composition of propolis from the Baha Region in Saudi Arabia. Czech Journal of Food Science, 36(2), 00-10.
- [27] Ghamdi, A.A., Bayaqoob, N.I., Rushdi, A.I., Alattal, Y., Simoneit, B.R., El-Mubarak, A.H., Al-Mutlaq, K.F. (2017). Chemical compositions and characteristics of organic compounds in propolis from Yemen. Saudi Journal of Biological Sciences, 24(5), 1094-1103.
- [28] Mohtar, L.G., Rodríguez, S.A., Nazareno, M.A. (2017). Comparative analysis of volatile compound profiles of propolis from different provenances. Journal of the Science of Food and Agriculture, 98(9), 3409-3415.
- [29] Taddeo, V.A., Epifano, F., Fiorito, S., Genovese, S. (2016). Comparison of different extraction methods and HPLC quantification of prenylated and unprenylated phenylpropanoids in raw Italian propolis. Journal of Pharmaceutical and Biomedical Analysis, 129, 219-223.
- [30] Netíková, L., Bogusch, P., Heneberg, P. (2013). Czech ethanol‐free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens. Journal of Food Science, 78(9), 1421-1429.
- [31] Arslan, S., Perçin, D., Silici, S., Er, Ö. (2010). Farklı çözücülerle hazırlanan propolis özütlerinin mutans streptokoklar üzerine in vitro antimikrobiyal etkisi. Sağlık Bilimleri Dergisi, 19(1), 68.
- [32] ISO 22196:2011, Plastics-Measurement of Antibacterial Activity on Plastics and Other Non-porous Surfaces. Geneva, Switzerland.
- [33] Bakkaloğlu, Z., Arıcı M. (2019). Farklı çözücülerle propolis ekstraksiyonunun toplam fenolik içeriği, antioksidan kapasite ve antimikrobiyal aktivite üzerine etkileri. Akademik Gıda, 17(4) 538-545.
- [34] Mascheroni, E., Guillard, V., Nalin, F., Mora, L., Piergiovanni, L. (2010). Diffusivity of propolis compounds in Polylactic acid polymer for the development of anti-microbial packaging films. Journal of Food Engineering, 98, 294-301.
- [35] Martí, M., Frígols, B., Serrano-Aroca, A. (2018). Antimicrobial characterization of advanced materials for bioengineering applications. The Journal of Visualized Experiments, 28(4), 138.
- [36] Japanese Standards Association (2000). Japanese Industrial Standard JIS Z 2801:2000. Antimicrobial products-test for antimicrobial activity and efficacy. Tokyo, Japan.