In Vitro Plant Regeneration of Libyan Wild Plants: Edible Species (Arbutus pavarii) and Endanger Species [Haplophyllum tuberculatum (Forsk.) Juss]

Yıl 2017, Cilt: 27 Sayı: 2, 127 - 132, 15.12.2017

Adel M. Elmaghabı Salem Hammud Elmunder Abugnıa

Öz

Protocol of
micropropagation of Arbutus pavarii and Haplophyllum
tuberculatum was successfully achieved. In vitro plant
regeneration of Arbutus pavarii was attempted using single nodal on MS
medium supplemented with different concentration of three various growth
regulators indole-3-butyric acid (IBA), Kinetin (K) and
N-Isopentenylaminopurine (2ip). Multiple shoots from single node were obtained
on MS medium with the 0.5 mg/l 2ip. While, faster improving of shoots elongation
was on MS contained 0.5 mg/lK. Explants obtained in
micropropagation step were used for rooting step under several treatments. The
best results were obtained when explants were sup-cultured on MS medium with 1
mg/l IBA. New plants were vigorous, of good quality and presented phenotypic
characters similar to mother plants. Micropropagation of Haplophyllum tuberculatum was achieved from sterilized single
nodal segments on MS medium supplemented with for different concentrations of
2,4-D for callus induction and other different hormones K and BA for
multiplication of auxiliary branches and rooting . The highest results of the
weight of callus were growing in MS medium containing 1 or 2 mg/l 2.4-D hormone
with maltose. Whereas, the axillary soothing was significantly proliferated on
MS medium supplemented 2 mg/ l^-1 K. Acclimation of plantlet was in
greenhouse. 

Anahtar Kelimeler

Micropropagation, growth regulators, Arbutus pavarii, Haplophyllum tuberculatum, in vitro culture

Kaynakça

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