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Entomopatojen nematod Heterorhabditis bacteriophora'nın (Rhabditida: Heterorhabditidae) kontrollü koşullar altında patates güvesi (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) üzerinde etkinliğinin belirlenmesi

Yıl 2021, Cilt: 31 Sayı: 2, 170 - 174, 15.12.2021
https://doi.org/10.18615/anadolu.1030016

Öz

Patates güvesi Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), tüm dünyada patates üreten ülkelerde verim kayıplarına neden olan önemli bir patates zararlısıdır. Bu zararlı populasyonu azaltmak için birçok mücadele yöntemi mevcuttur. Bunların başında kimyasal mücadele yöntemleri gelmektedir. Örnek olarak, Entomopatojenik nematodlar (EPN'ler), patates güvesi larvalarını kontrol etmek için kimyasal insektisitlere alternatif ve çevre dostu bir mücadele yöntemi olarak kullanılabilir. Bu çalışmada, Türkiye'deki Heterorhabditis bacteriophora (TOK-20) izolatlarının patates güvesinin son dönem (dördüncü dönem) larvalarına karşı etkinliğinin laboratuvar koşullarında araştırılmasını amaçlanmıştır. Deneyler, steril toprak karışımı ile 150 mililitrelik plastik kaplarda yapılmıştır. Dört nematod konsantrasyonu (0, 250, 500 ve 1000 IJ) doğrudan toprağa uygulanmıştır. Patates güvesi farklı konsantrasyonlarda H. bacteriophora (TOK-20) izolatına duyarlı bulunmuştur. Tüm dozlar kontrolden (su) daha etkili çıkmıştır. En yüksek ölüm oranı en etkili doz olan 1000 IJs konsantrasyonda görülmüş olup, sıcaklıklar arasında herhangi bir istatistiki farklılık görülmemiştir. Bu sonuçlar, H. bacteriophora (TOK-20) izolatının çok etkili olduğunu ve biyolojik mücadele programlarında patates güvesine karşı mücadelede kullanılabileceğini göstermiştir.

Kaynakça

  • Abbott, W.S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18: 265-267.
  • Anonim. 2008. Zirai Mücadele Teknik Talimatları. Cilt.3. Ankara.
  • Anonim. 2020. https://data.tuik.gov.tr/Bulten/ Index?p= Bitkisel-Uretim-Istatistikleri-2020-33737.
  • Belien, T. 2018. Entomopathogenic nematodes as biocontrol agents of insect pests in orchards. CAB Reviews 13, No. 058.
  • Chen, S.Y, D.W. Dickson and D.J. Mitchell. 1995. Effects of soil treatments on the survival of soil microorganisms. J Nematol 27(4S):661–663.
  • Golizadeh, A., N. Esmaeili, J. Razmjou, and H. Rafiee-Dastjerdi. 2014. Comparative life tables of the potato tuberworm, Phthorimaea operculella, on leaves and tubers of different potato cultivars. Journal of Insect Science 14 (42): 1-11.
  • Gulcu, B., H. Cimen, R.K. Raja, and S. Hazir. 2017. Entomopathogenic nematodes and their mutualistic bacteria: their ecology and application as microbial control agents. Biopestic Int1. 3 (2), 79–112.
  • Hassani-Kakhki, M., J. Karimi, and M. Hossein. 2013. Efficacy of entomopathogenic nematodes against potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae) under laboratory conditions. Biocontrol Science and Technology 23: 146-159.
  • Kaya, H.K., and S.P. Stock. 1997. Techniques in insect nematology. pp. 281-324. In: L. Lacey (Ed.). Manual of Techniques in Insect Pathology. Academic Press, San Diego, California, USA. Kepenekci, İ., A. Tülek, M. Alkan and S. Hazir. 2013. Biological control potential of native entomopathogenic nematodes against the potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) in Turkey. Pakistan Journal of Zoology 45(5): 1415-1422.
  • Laznik, Ž., and S. Trdan. 2012. Entomopathogenic nematodes (Nematoda: Rhabditida) in Slovenia: from tabula rasa to implementation into crop production systems. pp. 627-656. In: F. Perveen (Ed.). Insecticides - Advances ın Integrated Pest Management. Rijeka: Intech.
  • Mhatre, P.H., J. Patil, V. Rangasamy, K.L. Divya, S. Tadigiri, G. Chawla, A. Bairwa, and E.P. Venkatasalam. 2020. Biocontrol potential of Steinernema cholashanense (Nguyen) on larval and pupal stages of potato tuber moth, Phthorimaea operculella (Zeller). Journal of Helminthology Volume 94, 2020, e188. DOI: https://doi.org/10.1017/S0022149X20000723.
  • Moawad, S.S., M.M.E. Salah, H.M. Metwally, I.M. Ebadah, and Y.A. Mahmoud. 2018. Protective and curative treatments of entomopathogenic nematodes against the potato tuber moth, Phthorimaea operculella (Zell.). Bioscience Research 15(3): 2602-2610.
  • Mohamed, M.A., and H.C. Coppel. 1983. Mass rearing of the greater wax moth Galleria melonella (Lepidoptera: Galleridae) for small-scale laboratory. The Great Lakes entomologist 16 (4) 139-141.
  • Odendaal, D, M.F. Addison, and P. Antoinette. 2015. Entomopathogenic nematodes for the control of the codling moth (Cydia pomonella L.) in field and laboratory trials. Journal of Helminthology1(5):1-9.
  • Orakçı, M. 2021. Patates Güvesi [Phthorımaea Operculella (Zeller) (Lepıdoptera: Gelechııdae]’ne karşı Türkiye ve Kırgızistan’dan elde edilen bazı entomopatojen nematodların sera-saksı denemeleriyle etkinliğinin araştırılması. Yüksek lisans tezi. Tokat Gaziosmanpaşa Üniversitesi.
  • Robbins, R.T. and K.R. Barker. 1974. The effect of soil type, particle size, temperature, and moisture on reproduction of Belonolaimus longicaudatus. Journal of Nematology 6:1-6. Rondon, S.I. 2010. The potato tuberworm: a literature review of ıts biology, ecology and control. J.pot Res 87:149-166.
  • Shields, E.J., A. Testa, J.M. Miller, K.L. Flanders. 1999. Field efficacy and persistence ofthe entomopathogenic nematodes Heterorhabditis bacteriophora ‘Oswego’ and H.bacteriophora ‘NC’ on Alfalfa snout beetle larvae (Coleoptera: Curculionidae). Environmental Entomology 28: 128-136.
  • White, G.F. 1927. A method for obtaining infective nematode larvae from cultures. Science 66: 302-303.
  • Yan J.J., S.C. Sarkar, R.X. Meng, R. Stuart, and Y.L. Gao. 2020. Potential of Steinernema carpocapsae (Weiser) as a biological control agent against potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae). Journal of Integrative Agriculture 19(2): 389-393.

Determination of efficiency of entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) on the potato tuber moth (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) under controlled conditions

Yıl 2021, Cilt: 31 Sayı: 2, 170 - 174, 15.12.2021
https://doi.org/10.18615/anadolu.1030016

Öz

The potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), is an important pest of potato that causes yield losses in potato producing countries all over the world. Many management methods are available for reducing this pest populations. Chemical control methods are at the top of these. However, Entomopathogenic nematode (EPNs), can be used as a potential alternative to chemical insecticides to control potato tuber moth larvae as an eco-friendly management method. We aimed in this study to examine the efficacy of Turkish isolates of Heterorhabditis bacteriophora (TOK-20) against the last instar larvae (fourth stage) of the potato tuber moth under laboratory conditions. Experiments were conducted in 150 milliliter plastic cups with sterile soil mixture. Four nematode concentrations (0, 250, 500 and 1000 IJs) were applied directly to the soil. Potato tuber moth was susceptible to different concentrations of the H. bacteriophora (TOK-20) isolate. All doses were more effective than the control (water). The most effective was the 1000 IJs concentratio, providing a high mortality rate of the last instar larvae of potato tuber moth. No statistically significant difference was observed among temperatures. These results indicated that this H. bacteriophora (TOK-20) isolate is very efficient and could be used against potato tuber moth in biological control programs.

Kaynakça

  • Abbott, W.S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18: 265-267.
  • Anonim. 2008. Zirai Mücadele Teknik Talimatları. Cilt.3. Ankara.
  • Anonim. 2020. https://data.tuik.gov.tr/Bulten/ Index?p= Bitkisel-Uretim-Istatistikleri-2020-33737.
  • Belien, T. 2018. Entomopathogenic nematodes as biocontrol agents of insect pests in orchards. CAB Reviews 13, No. 058.
  • Chen, S.Y, D.W. Dickson and D.J. Mitchell. 1995. Effects of soil treatments on the survival of soil microorganisms. J Nematol 27(4S):661–663.
  • Golizadeh, A., N. Esmaeili, J. Razmjou, and H. Rafiee-Dastjerdi. 2014. Comparative life tables of the potato tuberworm, Phthorimaea operculella, on leaves and tubers of different potato cultivars. Journal of Insect Science 14 (42): 1-11.
  • Gulcu, B., H. Cimen, R.K. Raja, and S. Hazir. 2017. Entomopathogenic nematodes and their mutualistic bacteria: their ecology and application as microbial control agents. Biopestic Int1. 3 (2), 79–112.
  • Hassani-Kakhki, M., J. Karimi, and M. Hossein. 2013. Efficacy of entomopathogenic nematodes against potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae) under laboratory conditions. Biocontrol Science and Technology 23: 146-159.
  • Kaya, H.K., and S.P. Stock. 1997. Techniques in insect nematology. pp. 281-324. In: L. Lacey (Ed.). Manual of Techniques in Insect Pathology. Academic Press, San Diego, California, USA. Kepenekci, İ., A. Tülek, M. Alkan and S. Hazir. 2013. Biological control potential of native entomopathogenic nematodes against the potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) in Turkey. Pakistan Journal of Zoology 45(5): 1415-1422.
  • Laznik, Ž., and S. Trdan. 2012. Entomopathogenic nematodes (Nematoda: Rhabditida) in Slovenia: from tabula rasa to implementation into crop production systems. pp. 627-656. In: F. Perveen (Ed.). Insecticides - Advances ın Integrated Pest Management. Rijeka: Intech.
  • Mhatre, P.H., J. Patil, V. Rangasamy, K.L. Divya, S. Tadigiri, G. Chawla, A. Bairwa, and E.P. Venkatasalam. 2020. Biocontrol potential of Steinernema cholashanense (Nguyen) on larval and pupal stages of potato tuber moth, Phthorimaea operculella (Zeller). Journal of Helminthology Volume 94, 2020, e188. DOI: https://doi.org/10.1017/S0022149X20000723.
  • Moawad, S.S., M.M.E. Salah, H.M. Metwally, I.M. Ebadah, and Y.A. Mahmoud. 2018. Protective and curative treatments of entomopathogenic nematodes against the potato tuber moth, Phthorimaea operculella (Zell.). Bioscience Research 15(3): 2602-2610.
  • Mohamed, M.A., and H.C. Coppel. 1983. Mass rearing of the greater wax moth Galleria melonella (Lepidoptera: Galleridae) for small-scale laboratory. The Great Lakes entomologist 16 (4) 139-141.
  • Odendaal, D, M.F. Addison, and P. Antoinette. 2015. Entomopathogenic nematodes for the control of the codling moth (Cydia pomonella L.) in field and laboratory trials. Journal of Helminthology1(5):1-9.
  • Orakçı, M. 2021. Patates Güvesi [Phthorımaea Operculella (Zeller) (Lepıdoptera: Gelechııdae]’ne karşı Türkiye ve Kırgızistan’dan elde edilen bazı entomopatojen nematodların sera-saksı denemeleriyle etkinliğinin araştırılması. Yüksek lisans tezi. Tokat Gaziosmanpaşa Üniversitesi.
  • Robbins, R.T. and K.R. Barker. 1974. The effect of soil type, particle size, temperature, and moisture on reproduction of Belonolaimus longicaudatus. Journal of Nematology 6:1-6. Rondon, S.I. 2010. The potato tuberworm: a literature review of ıts biology, ecology and control. J.pot Res 87:149-166.
  • Shields, E.J., A. Testa, J.M. Miller, K.L. Flanders. 1999. Field efficacy and persistence ofthe entomopathogenic nematodes Heterorhabditis bacteriophora ‘Oswego’ and H.bacteriophora ‘NC’ on Alfalfa snout beetle larvae (Coleoptera: Curculionidae). Environmental Entomology 28: 128-136.
  • White, G.F. 1927. A method for obtaining infective nematode larvae from cultures. Science 66: 302-303.
  • Yan J.J., S.C. Sarkar, R.X. Meng, R. Stuart, and Y.L. Gao. 2020. Potential of Steinernema carpocapsae (Weiser) as a biological control agent against potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae). Journal of Integrative Agriculture 19(2): 389-393.
Toplam 19 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm Makaleler
Yazarlar

Mürşide Yağcı 0000-0003-2269-7649

Cenk Yücel Bu kişi benim 0000-0001-5223-9257

F. Dolunay Erdoğuş Bu kişi benim 0000-0001-9847-6100

F. Manolya Akbulut Bu kişi benim 0000-0003-3145-0808

Yayımlanma Tarihi 15 Aralık 2021
Gönderilme Tarihi 6 Mart 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 31 Sayı: 2

Kaynak Göster

APA Yağcı, M., Yücel, C., Erdoğuş, F. D., Akbulut, F. M. (2021). Determination of efficiency of entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) on the potato tuber moth (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) under controlled conditions. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, 31(2), 170-174. https://doi.org/10.18615/anadolu.1030016
AMA Yağcı M, Yücel C, Erdoğuş FD, Akbulut FM. Determination of efficiency of entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) on the potato tuber moth (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) under controlled conditions. ANADOLU. Aralık 2021;31(2):170-174. doi:10.18615/anadolu.1030016
Chicago Yağcı, Mürşide, Cenk Yücel, F. Dolunay Erdoğuş, ve F. Manolya Akbulut. “Determination of Efficiency of Entomopathogenic Nematode Heterorhabditis Bacteriophora (Rhabditida: Heterorhabditidae) on the Potato Tuber Moth (Phthorimaea Operculella (Zeller)) (Lepidoptera: Gelechiidae) under Controlled Conditions”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 31, sy. 2 (Aralık 2021): 170-74. https://doi.org/10.18615/anadolu.1030016.
EndNote Yağcı M, Yücel C, Erdoğuş FD, Akbulut FM (01 Aralık 2021) Determination of efficiency of entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) on the potato tuber moth (Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) under controlled conditions. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 31 2 170–174.
IEEE M. Yağcı, C. Yücel, F. D. Erdoğuş, ve F. M. Akbulut, “Determination of efficiency of entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) on the potato tuber moth (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) under controlled conditions”, ANADOLU, c. 31, sy. 2, ss. 170–174, 2021, doi: 10.18615/anadolu.1030016.
ISNAD Yağcı, Mürşide vd. “Determination of Efficiency of Entomopathogenic Nematode Heterorhabditis Bacteriophora (Rhabditida: Heterorhabditidae) on the Potato Tuber Moth (Phthorimaea Operculella (Zeller)) (Lepidoptera: Gelechiidae) under Controlled Conditions”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 31/2 (Aralık 2021), 170-174. https://doi.org/10.18615/anadolu.1030016.
JAMA Yağcı M, Yücel C, Erdoğuş FD, Akbulut FM. Determination of efficiency of entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) on the potato tuber moth (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) under controlled conditions. ANADOLU. 2021;31:170–174.
MLA Yağcı, Mürşide vd. “Determination of Efficiency of Entomopathogenic Nematode Heterorhabditis Bacteriophora (Rhabditida: Heterorhabditidae) on the Potato Tuber Moth (Phthorimaea Operculella (Zeller)) (Lepidoptera: Gelechiidae) under Controlled Conditions”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, c. 31, sy. 2, 2021, ss. 170-4, doi:10.18615/anadolu.1030016.
Vancouver Yağcı M, Yücel C, Erdoğuş FD, Akbulut FM. Determination of efficiency of entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) on the potato tuber moth (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) under controlled conditions. ANADOLU. 2021;31(2):170-4.
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