Diagnostic Importance of Syndecan-1(CD138) in Chronic Endometritis of Dogs

Abstract

In this study, the role of Syndecan-1 in the diagnosis of chronic endometritis, which is an important health problem for dogs and mostly causes infertility, was investigated. In addition, comparisons of Syndecan-1 and HE staining methods in the diagnosis of chronic endometritis were made. Generally, the prognosis is not good because the diagnosis is difficult in chronic endometritis, where no clinical symptoms can be observed. For this, early diagnosis with a reliable method in chronic forms of endometritis is very important. The presence of plasmocytes in the histopathological examination, which is one of the various diagnostic methods of endometritis, is a distinctive feature for the chronic form of the inflammatory character. Plasmocytes, which can be detected by histopathological staining methods can also be detected by immunohistochemical staining of the Syndecan-1 receptors that specific to plasmocytes at the same time. For this study, a sufficient number of female dog uterus was obtained in and around Van province. Hematoxylin-eosin (HE) staining of uterine tissues for histopathology and Syndecan-1 staining for immunohistochemical examination were performed. In the examinations made, 18 of 57 uterus samples with HE staining; With Syndecan-1, 21 of them were diagnosed with chronic endometritis. In the result of working; With Syndecan-1 staining, it was observed that plasmocytes could be selected more easily and clearly than HE. In addition, it was determined that Syndecan-1 is a reliable and practical method in the diagnosis of chronic endometritis with this study, which was performed for the first time in dogs.

Keywords

• Chronic endometritis
• Dog
• Hematoxylin-Eosin
• Syndecan-1

Köpeklerin Kronik Endometritislerinde Sydecan- 1(CD138)’in Diagnostik Önemi

Öz

Bu çalışmada köpekler için önemli bir sağlık problemi olan ve çoğunlukla infertiliteye yol açan kronik endometritislerin tanısında Syndecan-1’in rolü araştırılmıştır. Ayrıca Syndecan-1 ve hematoksilen-eozin (HE) boyama yöntemlerinin kronik endometritislerin tanısındaki karşılaştırmaları yapılmıştır. Genellikle herhangi bir klinik semptom gözlenemeyen kronik endometritislerde tanı güç olduğundan prognoz iyi değildir. Bunun için endometritislerin kronik formlarında güvenilir bir yöntemle yapılan erken tanı oldukça önemlidir. Endometritislerin çeşitli tanı yöntemlerinden biri olan histopatolojik muayenede plazmositlerin varlığı yangı karakterinin kronik formu için ayırıcı bir özelliktir. Histopatolojik boyama yöntemleriyle belirlenebilen plazmositler, aynı zamanda plazmositlere spesifik Syndecan-1 reseptörünün immünohistokimyasal boyama yöntemiyle de saptanabilir. Bu çalışma için Van ili ve çevresinde 67 adet dişi köpek uterusu temin edildi. Uterus dokularının histopatoloji için hematoksilen-eozin (HE) ve immünohistokimyasal inceleme için Syndecan-1 boyamaları gerçekleştirildi. Yapılan incelemelerde 57 adet endometritisli uterus örneklerinden HE boyamayla 18’ine; Syndecan-1 ile 21’ine kronik endometritis tanısı konuldu. Çalışma sonucunda; HE’nin aksine Syndecan-1 boyama ile plazmositlerin daha kolay ve net bir şekilde seçilebildiği görüldü. Ayrıca köpeklerde ilk defa yapılan bu çalışmayla kronik endometritislerin tanısında Syndecan-1’in güvenilir ve pratik bir yöntem olduğu tespit edildi.

Anahtar Kelimeler

• Hematoksilen-Eozin
• Köpek
• Kronik endometritis
• Syndecan-1.

Kaynakça

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