Doğal Enfekte Köpek Testislerinde Leptospiral Antijenlerinin İmmunohistokimyasal Olarak Araştırılması

Yıl 2021, Cilt: 16 Sayı: 3, 236 - 242, 30.12.2021

İsmail Bolat , Yavuz Sağlam , Serkan Yıldırım

https://doi.org/10.17094/ataunivbd.928819

Öz

Leptospirozis dünyada ve ülkemizde yaygın olarak gözlenen zoonoz bir hastalıktır. Hastalık insanların yanı sıra, sığır, koyun, kedi, köpek ve diğer evcil hayvanları da etkilemektedir. İnsanlarda ciddi sağlık problemlerine, hayvanlarda da önemli ekonomik kayıplara sebep olan hastalıkta, bazı canlılar vücutlarında etken olmasına rağmen herhangi bir klinik semptom göstermeden hastalığı yayarlar. Bu nedenle hastalığın farklı hayvan türlerinde sürekli araştırılması önem arz etmektedir. Hastalıkla ilgili dünya genelinde insanlarda ve hayvanlarda yapılmış birçok çalışma mevcuttur. Türkiye’ de ise hastalıkla ilgili yapılan çalışmaların çoğu serolojik çalışmalar olup, farklı hayvan türlerinde immunohistokimyasal çalışmalar da yapılmıştır. Hastalığın tanısında çalışmanın örneklerinin sağlandığı Erzurum bölgesinde immunohistokimyasal yöntemler ile sığır ve koyun abortları üzerine çalışmalar yapılmış olmasına rağmen, köpeklerde bir çalışmaya rastlanılmamıştır. Bu çalışmada, doğal enfekte köpeklerin testis doku örneklerinde Leptospira interrogans (L. İnterrogans) antijenlerinin immunohistokimyasal yöntemler ile dokudaki varlıkları ve yerleşim yerlerinin belirlenmesi amaçlanmıştır. Çalışmada makroskobik lezyon aranmaksızın toplanan 30 adet köpek testis dokusu örneği immunohistokimyasal yöntem ile incelendi. İnceleme sonucunda köpek testis doku örneklerinde %26.66 (8 adet) oranında L. İnterrogans antijen varlığı tespit edildi. Araştırma sonuçları leptospirozisin köpeklerde de önemli sağlık sorunu oluşturduğunu göstermiştir.

Anahtar Kelimeler

İmmunohistokimya, Köpek, Leptospira interrogans, Leptospirozis, Testis

Immunohistochemically Investigation of Leptospiral Antigens in Naturally Infected Dog Testicles

Öz

Leptospirosis is a zoonotic disease common in the world and in our country. The disease affects people as well as cattle, sheep, cats, dogs, and other domestic animals. In the disease, which causes serious health problems in humans and significant economic losses in animals, some living things spread the disease without showing any clinical symptoms, although they are causative agents in their bodies. For this reason, it is important to constantly investigate the disease in different animal species. There are many studies on the disease in humans and animals worldwide. In Turkey, most of the studies are serological, but immunohistochemical studies were also conducted in different animal species. Although there have been studies on cattle and sheep abortions with immunohistochemical methods in the Erzurum region, where the study samples were provided for the diagnosis of the disease, no study was found in dogs. This study was aimed to determine the presence and localization of Leptospira interrogans (L. İnterrogans) antigens in testicular tissue samples of naturally infected dogs with immunohistochemical methods. In the study, 30 dog testicular tissue samples collected without looking for macroscopic lesions were examined using the immunohistochemical method. As a result of the examination, 26.66% (8) L. İnterrogans antigens were detected in canine testicular tissue samples. The research results showed that leptospirosis is an important health problem in dogs.

Anahtar Kelimeler

Citrus extract, Diarrhea, Giardiasis, Small ruminant, Treatment

Kaynakça

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