Sarcoptes Scabiei ile Enfeste Koyunlarda İnterlökin ve Vitamin Düzeylerinin Değerlendirilmesi

Yıl 2022, Cilt: 17 Sayı: 1, 11 - 15, 29.04.2022

Pelin Fatoş Polat Dinçer Ömer Faruk Gökcecik Alper Başa

Öz

Çalışmanın amacı S. scabiei ile doğal enfekte koyunlarda vitamin E, 25-hydroxyvitamin D3 (25(OH)D3), interleukin-2 (IL-2) ve interleukin-4 (IL-4) seviyelerinin tespit edilmesi ayrıca baskın sitokin yanıtının belirlenerek 25(OH)D3 ile ilişkisinin araştırılmasıdır. Bu amaçla, 1-4 yaşları arasında S. scabiei ile doğal enfekte, kabuklu yaralara sahip 20 ve kontrol grubu olarak aynı yaş aralığında sağlıklı 10 koyun kullanıldı. Her hayvandan 5 ml kan alınıp serumları analiz edilinceye kadar -20 °C’de saklandı. Uyuz akarlarının tespiti için kazıntı alınarak mikroskopta muayene edildi. Serumda IL-2 ve IL-4 tayini enzyme-linked immunosorbent assay (ELİSA), E vitamini analizleri için high-performance liquid chromatography (HPLC) ve 25 (OH)D3 tayini için İmmunoassay kullanıldı. E vitamini ve 25(OH)D3 seviyelerinin enfekte koyunlarda kontrol grubuna göre istatistiksel olarak azaldığı (P < ,05) görüldü. Enfekte hayvanların IL-4 seviyesi kontrol grubuna göre istatistiksel olarak artış (P < ,05) gösterirken, IL-2 seviyesinde gruplar arasında istatistiksel fark tespit edilemedi (P > ,05). Sonuç olarak, S. scabiei ile enfekte koyunlarda meydana gelen kabuklu uyuzlarda serum E vitamini ve 25(OH)D3 düzeylerinin azaldığı, T lenfositlerin kritik rolünün olduğu ve immun yanıtın Th2 yönünde olduğu görülmüştür.

Anahtar Kelimeler

E Vitamini, interlökin, 25-hidroksivitamin D.

Evaluation of Interleukin and Vitamin Levels in Sheep Infested with Sarcoptes Scabiei

Öz

This study determined the levels of Vitamin E, 25-hydroxyvitamin D3 (25(OH)D3), interleukin-2 (IL-2), and interleukin-4 (IL-4) in sheep naturally infected with Sarcoptes scabiei (S. scabiei). It also determined the dominant cytokine response and investigated its relationship with 25(OH)D3. For this purpose, 20 sheep between the ages of 1-4 years, naturally infected with S. scabiei, with crusted wounds were compared with 10 healthy sheep in the same age range. Five ml of blood was collected from each animal and stored at -20 °C until the serum was analyzed. To detect scabies, scrapings were taken and examined under a microscope. Enzyme-linked immunosorbent assay (ELISA) was to determine IL-2 and IL-4 serum levels, high-performance liquid chromatography (HPLC) for Vitamin E analysis, and immunoassay for 25(OH)D3 determination. Vitamin E and 25(OH)D3 levels were significantly lower in infected sheep compared to the control group (P < .05) whereas the IL-4 level of the infected animals was significantly higher (P < .05) compared to the control group. There was no significant difference between the groups for IL-2 level (P > .05). In conclusion, serum vitamin E and 25(OH)D3 levels were lower in crusted scabies in sheep infected with S. scabiei, T lymphocytes had a critical role, and the immune response was in the direction of Th2.

Anahtar Kelimeler

Interleukin, vitamin E, 25-hydroxyvitamin D.

Kaynakça

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