Bazı Bitki Patojeni Funguslara Karşı Humulus lupulus L. Bitki Ekstraktının Antifungal Aktivitesi

Öz

Bu çalışmada, Tokat ekolojik koşullarda yetişen Humulus lupulus L.(Şerbetçiotu) bitkisinin çiçeklerinden elde edilen methanol ve n-hexan ekstraktlarının Sclerotinia sclerotiorum ve Fusarium oxysporum f.sp. lycopersici (Fol) patojenlerine karşı antifungal aktivitesi in vitro şartlarda araştırılmıştır. Aktivite çalışmalarında, agar difüzyon yöntemi kullanılmıştır. Methanol ve n-Hexan ekstraktlarının patojenlere karşı, 0.1, 0.5, 1 ve 2 mg/ml dozlarının Miselyum Gelişimi (MG), Miselyum Gelişim Engelleme (MGE) etkinlik düzeyleri belirlenmiş ve letal doz (LD₅₀) hesaplanmıştır. Patojenler üzerine; hexan ekstraktı, metanol ekstraktına göre daha etkili olduğu bulunmuştur. MG için; Hexan ekstraktının 2 mg/ml dozunda Sc ve Fol’e karşı sırasıyla %90 ile %60 oranında etkili olduğu, yine MG için; methanol ekstraktında 2 mg/ml dozunda Sc ve Fol’e karşı sırasıyla %84 ile %83 oranında engellediği belirlenmiştir. LD₅₀ hexan ve methanol ekstraktları için Sc için; sırasıyla 0.021 ile 0.193 mg/ml, Fol için; sırasıyla 0.616 ile 0.273 mg/ml olarak hesaplanmıştır. Çalışma sonucunda; H.lupulus çiçeklerinden elde edilen Methanol ve n-Hexan ekstraktlarının in vitro şartlarda Sclerotinia sclerotiorum ve Fusarium oxysporum f.sp. lycopersici üzerine fungusidal aktivitelerinin olduğu belirlenmiştir.

Anahtar Kelimeler

• Antifungal etki
• Humulus lupulus
• Sclerotinia sclerotiorum
• Fusarium oxysporum f.sp. lycopersici

Antifungal Activity of Humulus lupulus L. Flower Extract Against Some Plant Pathogen Fungi

Abstract

In this study, methanol and n-hexan extracts obtained from flowers of Humulus lupulus L. (Hops) grown in Tokat ecological conditions were investigated by Sclerotinia sclerotiorum (Ss) and Fusarium oxysporum f.sp. lycopersici (Fol) pathogens was investigated antifungal activity against in vitro condition. Agar diffusion method was used for activity studies. Mycelial Growth (MG), Mycelium Growth Inhibition (MGE) activity levels of 0.1, 0.5, 1 and 2 mg/ml doses of methanol and n-hexan extracts against pathogens were determined and lethal dose (LD₅₀) was calculated. Generally, on pathogens; n-hexane extract was found to be more effective than methanol extract. For MG; n-hexan extract was effective at a dose of 2 mg/ml against Sc and Fol to 90% and 60% respectively. It was determined that it inhibited Sc and Fol at a dose of 2 mg/ml in methanol extract to 84% and 83%, respectively. LD₅₀ was calculated for Sc for n-hexane and methanol extracts; 0.021 to 0.193 mg/ml, respectively, for Fol; 0.616 to 0.273 mg/ml, respectively. In the results of research; methanol and n-Hexan extracts obtained from H.lupulus flowers in vitro conditions Sclerotinia sclerotiorum and Fusarium oxysporum f.sp. lycopersici on fungicidal activities.

Keywords

• Antifungal effect
• Humulus lupulus
• Sclerotinia sclerotiorum
• Fusarium oxysporum f.sp. lycopersici

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