Tokat ve Nevşehir Yörelerinde Yetişen Narince Üzümü Ve Üzüm Şırasının Spontan Fermantasyonu Sırasında Maya Popülasyonunun PZR–RFLP Teknikleri İle Belirlenmesi

Öz

Bu çalışmada, 2015 yılında Tokat ve Nevşehir yörelerinden bağbozumu dönemlerinde hasat edilen Narince üzümlerinin spontan fermantasyonu sırasında ortamda bulunan S.cerevisiae ve Saccharomyces spp. olmayan mayaların popülasyonu incelenmiştir. Mayaların moleküler yöntemle tanımlanmasında PZR–RFLP yöntemi kullanılmıştır. PZR yönteminde mayalar öncelikle 5.8S rRNA’larında bulunan ITS gen bölgelerinin boyutlarına göre gruplandırılmış, daha sonra 26S rRNA’larına ait D1/D2 gen bölgeleri ile yapılan dizi analizi sonuçlarıyla mayalar tür düzeyinde tanımlanmışlardır. Narince üzümlerinden ve üzüm şırasının spontan fermantasyonu sırasında ortamdan 189 adet maya izole edilmiştir. Tokat yöresi hasat üzümlerinden yalnızca H’spora uvarum, Nevşehir yöresi hasat üzümlerinden ise yalnızca S. cerevisae izole edilmiştir. Narince şırasının spontan fermantasyonu sırasında ise H’spora uvarum, H’spora guilliermondii, S.cerevisiae, T.delbrueckii, W.anomalus, P.kluyveri, I.terricola, I.orientalis, P.occidentalis, L.thermotolerans, Metchinikowia spp. ve C.zemplinina, türleri tanımlanmıştır.

Anahtar Kelimeler

• Narince
• Saccharomyces cerevisiae
• non–Saccharomyces
• PZR–RFLP
• spontan fermantasyon

Determination of Yeast Population by PCR–RFLP Techniques Isolated From Spontaneous Fermentation and Narince Grapes Grown in Tokat and Nevşehir

Öz

In this study S.cerevisiae and non–Saccharomyces populations were investigated during spontaneous alcoholic fermentation of Narince grapes grown in Tokat and Nevşehir harvested in 2015 vintage. Yeasts were identified by PCR–RFLP analysis of the 5.8 ITS rRNA region and sequence information for the D1/D2 domains of the 26S gene. During spontaneous fermentation of Narince, 189 yeasts were isolated. H’spora uvarum was only yeast isolated from Narince grapes obtained from Tokat region, however only S.cerevisiae was isolated from Cv. Narince harvested from Nevşehir region. During spontaneous fermentation of Narince grapes H’spora uvarum, H’spora guilliermondii, S.cerevisiae, T.delbrueckii, W.anomalus, P.kluyveri, I.terricola, I.orientalis, P.occidentalis, L.thermotolerans, Metchinikowia spp. and C.zemplinina species were identified.

Anahtar Kelimeler

• Narince
• Saccharomyces cerevisiae
• non–Saccharomyces
• PCR–RFLP
• spontaneous fermentation

Kaynakça

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