Phenolic compound content and antioxidant activity of different polarity solvent extracts of Maraş tobacco combined with various plant species

Yıl 2025, Cilt: 6 Sayı: 2, 107 - 113, 30.08.2025

İbrahim Sefa Okumuş , Duygu Mısırlı , Mahfuz Elmastas

https://doi.org/10.53445/batd.1767966

Öz

This study aims to comparatively evaluate the phenolic compound contents and antioxidant activities of extracts prepared with solvents of different polarities (ethanol:water and water) from Maraş tobacco (Nicotiana rustica), which is widely consumed in the Kahramanmaraş region of Türkiye, in combination with oak ash and poplar ash traditionally used together with it. For this purpose, prepared Maraş tobacco mixtures (Nicotiana rustica + oak ash [NRM] and Nicotiana rustica + poplar ash [NRK]) were subjected to extraction using different solvents, and the antioxidant capacities of the resulting extracts were analyzed by the DPPH• radical scavenging activity assay and the Cupric Ion Reducing Antioxidant Capacity (CUPRAC) method. Four different extracts (NRM-water extract, NRK-water extract, NRM-ethanol:water extract, and NRK-ethanol:water extract) were prepared, and their phenolic compound profiles were determined and evaluated using High-Performance Liquid Chromatography with Photodiode Array Detection (HPLC-PDA). The results revealed that ethanol:water extracts exhibited higher phenolic compound content and antioxidant capacity compared to water extracts. In particular, the NRM-ethanol:water extract demonstrated the highest activity in both antioxidant assays. These findings indicate that the antioxidant activity of Maraş tobacco can vary significantly depending on the type of plant ash combination and the solvent used.

Anahtar Kelimeler

Maraş tobacco , Nicotiana rustica , decoction , ethanol:water extract , antioxidant activity , HPLC , phenolic compound

Farklı bitki türleri ile kombine edilen Maraş otunun farklı polaritedeki çözücü ekstrelerinin fenolik bileşik içeriği ve antioksidan aktivitesi

Öz

Bu çalışma, Türkiye’nin Kahramanmaraş bölgesinde yaygın olarak tüketilen Maraş otu (Nicotiana rustica) ile geleneksel olarak birlikte kullanılan meşe ve kavak külü kombinasyonlarının, farklı polaritedeki çözücüler (etanol:su ve su) ile hazırlanmış ekstrelerinin fenolik bileşik içeriklerinin ve antioksidan aktivitelerinin karşılaştırmalı olarak değerlendirilmesini amaçlamaktadır. Bu amaçla, hazırlanan Maraş otu karışımları (Nicotiana rustica + meşe külü [NRM] ve Nicotiana rustica + kavak külü [NRK]) farklı çözücüler kullanılarak ekstraksiyon işlemine tabi tutulmuş; elde edilen ekstrelerin antioksidan kapasiteleri, DPPH• radikal giderme aktivitesi ve Bakır(II) İyonu İndirgeyici Antioksidan Kapasite (CUPRAC) yöntemleriyle analiz edilmiştir. Çalışmada dört farklı ekstre (NRM-su ekstresi, NRK-su ekstresi, NRM-etanol:su ekstresi, NRK-etanol:su ekstresi) hazırlanmış; fenolik bileşik profilleri Yüksek Performanslı Sıvı Kromatografisi – Fotodiyot Dizi Dedektörü (HPLC-PDA) ile belirlenerek değerlendirilmiştir. Bulgularda, etanol:su ekstrelerinin su ile hazırlanan ekstreye kıyasla daha yüksek fenolik bileşik içeriğine ve antioksidan kapasiteye sahip olduğu gözlemlenmiştir. Özellikle NRM-etanol:su ekstresi, her iki antioksidan testinde de en yüksek aktiviteyi göstermiştir. Elde edilen sonuçlarda, Maraş otunun farklı bitki külü kombinasyonları ve çözücü tipleri ile antioksidan aktivitesinin önemli ölçüde değişebileceği görülmüştür.

Anahtar Kelimeler

Maraş otu , Nicotiana rustica , dekoksiyon , etanol:su ekstre , antioksidan aktivite , HPLC , fenolik bileşik

Kaynakça

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