Development of a pH-Sensitive Chitosan–Anthocyanin Smart Film and Applicability to Various Perishable Foods

Yıl 2025, Cilt: 40 Sayı: 2, 445 - 459, 30.12.2025

Emine Aytunga Arık Kibar , Hatice İmge Oktay Başeğmez

Öz

This study reports the development of a pH-sensitive smart film based on chitosan incorporated with pressurized liquid extraction-derived anthocyanins from blackcurrant pomace. The formulation was optimized using Response Surface Methodology with a Central Composite Design, and the optimized film was evaluated across a wide pH range (1–11). Color responses (ΔE), calculated from L*, a*, b* values, were modelled using quadratic regression (R²=0.91). The pH–ΔE model was applied to literature-reported spoilage pH ranges of perishable foods, enabling predictive color transitions. These findings reveal that the developed chitosan–anthocyanin film can serve as a versatile smart label for freshness monitoring and that the mathematical model can be adapted to mobile applications, enabling consumer-oriented digital freshness assessment.

Anahtar Kelimeler

Chitosan film , anthocyanin , intelligent packaging , pH sensitivity

Etik Beyan

The authors declare that there are no ethical conflicts regarding this study.

Destekleyen Kurum

TÜBİTAK MAM

Teşekkür

This study was supported by TÜBİTAK MAM internal funding. The authors thank TUBITAK MAM for their support.

Antosiyanin–Kitosan Tabanlı Akıllı Film Geliştirilmesi ve Çabuk Bozulabilen Gıdalara Uygulanabilirliğinin Değerlendirilmesi

Öz

Bu çalışmada siyah frenk üzümü posasından basınçlı sıvı ekstraksiyonu ile elde edilen antosiyaninlerin kitosan matriksine entegre edilmesiyle pH-duyarlı akıllı bir film geliştirilmiş ve bileşimi RSM tabanlı Merkezi Kompozit Tasarım ile optimize edilmiştir. Optimum film 1–11 pH aralığında test edilmiş, L*, a*, b* değerlerinden hesaplanan ΔE ikinci dereceden regresyon ile modellenmiştir (R²=0.91). Model, çabuk bozulabilen gıdaların literatürde belirtilen tazelik/bozulma pH aralıklarına uygulanmış ve renk geçişlerinin tahmini yapılmıştır. Bu sonuçlar, geliştirilen filmin çok çeşitli bozulabilir gıdalar için ayarlanabilir bir akıllı etiket bileşeni olarak kullanılabileceğini ve oluşturulan matematiksel modelin mobil uygulamalara entegre edilerek tüketiciye sayısal tazelik değerlendirmesi sunma potansiyeli taşıdığını göstermektedir.

Anahtar Kelimeler

Kitosan film , antosiyanin , akıllı ambalaj , pH duyarlılığı

Etik Beyan

Yazarlar bu çalışma ile ilgili herhangi bir etik çatışmanın bulunmadığını beyan etmektedir.

Destekleyen Kurum

TÜBİTAK MAM

Teşekkür

Bu çalışma TÜBİTAK MAM iç fonları ile desteklenmiştir. Yazarlar TÜBİTAK MAM’a destekleri için teşekkür eder.

Kaynakça

• Alegbeleye, O. O., Odeyemi, O. A., Strateva, M., Stratev, D. (2022) Microbial spoilage of vegetables, fruits and cereals. Applied Food Research 2(1):100122.
• Anonymous (t.y.) pH Values of Common Foods and Ingredients. Kansas State University, Extension Food Safety. https://cottonwood.k-state.edu/documents/fcs-docs/pH%20chart.pdf, Accessed 05 December 2025.
• Barth, M., Hankinson, T. R., Zhuang, H., Breidt, F. (2009) Microbiological spoilage of fruits and vegetables. W. H. Sperber, M. P. Doyle (Ed.), 239-270, Compendium of the Microbiological Spoilage of Foods and Beverages, Springer, New York.
• Basegmez, H. I. O., Povilaitis, D., Kitrytė, V., Kraujalienė, V., Šulniūtė, V., Alasalvar, C., Venskutonis, P. R. (2017) Biorefining of blackcurrant pomace into high value functional ingredients using supercritical CO2, pressurized liquid and enzyme assisted extractions. The Journal of Supercritical Fluids 124:10-19.
• da Silva Ponciano, C., Gonzaga, F. C., de Oliveira, C. P. (2025) Smart packaging based on chitosan acting as indicators of changes in food: A technological prospecting review. International Journal of Biological Macromolecules 145722.
• Fan, L., Yu, R., Xu, L., Li, H., Jiang, S. (2024) Application of visual intelligent labels for assessing freshness of chilled beef during storage. Food Chemistry 437:137720.
• FAO. (2022) The State of Food Security and Nutrition in the World 2022: Repurposing food and agricultural policies to make healthy diets more affordable. FAO, Rome. https://www.fao.org/3/cc0639en.pdf, Accessed 05 December 2025.
• Marappan, G., Liu, D., Li, R., Zhang, H. (2025) Natural pigment-based pH/gas-sensitive intelligent packaging films for freshness monitoring of meat and seafood. Food Reviews International 41(1):1-29.
• Masfufah, F. I. S., Ngadiwiyana, N., Yassaroh, Y., Wulandari, R., Prasetya, N. B. A. (2025) Antibacterial and pH-Responsive chitosan/glutaraldehyde film with red cabbage anthocyanins for meat freshness detection. Trends in Sciences 22(9):10254.
• Mutmainna, I., Tahir, D., Gareso, P. L., Suryani, S. (2025) Development of PVA–chitosan based smart packaging with the addition of red cabbage anthocyanin extract and copper-based metal-organic material (Cu-MOF). International Journal of Biological Macromolecules 313:144205.
• Nami, M., Taheri, M., Siddiqui, J., Deen, I. A., Packirisamy, M., Deen, M. J. (2024) Recent progress in intelligent packaging for seafood and meat quality monitoring. Advanced Materials Technologies 9(12):2301347.
• Prasetya, N. B. A., Masfufah, F. I. S., Ngadiwiyana, N., Yassaroh, Y., Wulandari, R. (2025) pH-sensitive chitosan/graphene oxide films enriched with red cabbage anthocyanins for visual spoilage detection in chicken meat. International Journal of Biological Macromolecules 147866.
• Sallam, K. I. (2004) Effects of trisodium phosphate and sodium chloride dipping on the microbial quality and shelf life of refrigerated tray-packaged chicken breasts. Food Microbiology 21(3):293-301.
• Shaddel, R., Alighadri, T., Alizadeh-Sani, M., Zahedi, Y., Rhim, J. W. (2025) Production and properties of smart packaging film based on gelatin-chitosan containing Echium amoenum anthocyanins and reinforced with crosslinkers for fish packaging. International Journal of Biological Macromolecules 145910.
• Timur, M., Paşa, A. (2018) Synthesis characterization, swelling, and metal uptake studies of aryl cross-linked chitosan hydrogels. ACS Omega 3(12):17416-17424.
• USDA ARS. (2024) pH of Selected Foods. Pathogen Modeling Program, U.S. Department of Agriculture, Agricultural Research Service. https://www.ars.usda.gov, Accessed 05 December 2025.
• Wang, Y., Kong, J., et al. (2023) Multi-functional pH-sensitive active and intelligent packaging film based on highly cross-linked zein. Food Chemistry 404:134679.
• Westlake, J. R., Burrows, A. D., Xie, M. (2025) Turmeric and red cabbage extract composites with chitosan as natural ingredient-based pH-responsive intelligent packaging strips. ACS Food Science & Technology 5(8):3159-3168.
• Wu, Y., Li, Z., Wu, Y., Chen, S., Zhao, Y., Li, C., Wang, Y. (2025) Chitosan titanium dioxide emulsion incorporated pH-responsive active packaging film: Real-time freshness monitoring and targeted fishy odor control. Carbohydrate Polymers 369:124317.
• Zhai, X., Xue, Y., Sun, Y., Ma, X., Ban, W., Marappan, G., Katona, J. (2025) Colorimetric food freshness indicators for intelligent packaging: Progress, shortcomings, and promising solutions. Foods 14(16):2813.
• Zhang, L., Fan, G., Khan, M. A., Xia, X., Zhou, X., Ma, L., Wang, J., Yan, Z. (2024) Recent advances in pH-sensitive indicator films based on natural colorants for smart monitoring of food freshness: A review. Critical Reviews in Food Science and Nutrition 64(33):12800-12819.