Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri

Semin Gedikli; Emin Şengül

doi:10.5798/dicletip.534825

Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri

Öz

Amaç: Siklofosfamid (CYP), karaciğer ve akciğer gibi birçok organda toksisiteye neden olur. Pek çok çalışmada bazı antioksidanların CYP'nin yan etkilerine karşı koruyucu etkileri olduğu gösterilmiştir. Bu çalışmada, kuersetinin histolojik ve biyokimyasal yöntemler kullanılarak sıçanlarda CYP ile indüklenen hepatotoksisite üzerindeki koruyucu etkisinin araştırılması amaçlanmıştır.

Yöntemler: Otuz Sprague-Dawley erkek sıçan 5 gruba ayrıldı. Kontrol grubuna 7 gün boyunca intragastrik olarak mısıryağı verildi. CYP grubuna 7 gün intragastrik olarak mısır yağı verildi ve 7. günde intraperitoneal olarak CYP (200 mg/kg) uygulandı. 7 gün boyunca Q50+CYP ve Q100+CYP gruplarına sırasıyla kuersetin verildi ve 7. günde tek doz CYP (200 mg/kg) uygulandı. Q100 grubuna günde 100 mg/kg dozda kuersetin verildi. 8. günde biyokimyasal ve histopatolojik incelemeler için kan örnekleri ve karaciğer dokuları alındı.

Bulgular: MDA seviyesinin kontrol grubu ile karşılaştırıldığında CYP grubunda belirgin olarak yüksek olduğunu ve kuersetin uygulaması ile azaldığını tespit ettik. SOD ve GSH düzeyleri CYP grubunda kontrol, Q50+CYP, Q100+CYP ve Q100 gruplarına göre azalmıştı. Histolojik analizlerde CYP grubunda sinüsoidal dilatasyon, mononükleer hücre infiltrasyonu ve vasküler konjesyon gözlenirken, bu dejeneratif değişikliklerin kuersetin uygulaması ile azaldığı tespit edildi. TUNEL yönteminde, CYP grubunda kontrol grubuna kıyasla fazla sayıda TUNEL pozitif hepatosit tespit edildi. Ayrıca Bax ve Caspase-3 immunpozitivitesi açısından CYP grubunda diğer gruplara oranla imünpozitiflik fazla iken, Bcl-2 immunpozitivitesi CYP grubunda diğer gruplardan daha düşüktü.

Sonuç: Elde ettiğimiz sonuçlar, kuersetinin siklofosfamidle indüklenen hepatotoksisite üzerinde koruyucu etkiye sahip olduğunu göstermektedir.

Anahtar Kelimeler

Siklofosfamid,Kuersetin,Karaciğer

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Semin Gedikli ^*
0000-0001-8238-7226

Emin Şengül Bu kişi benim
0000-0001-8238-7226

Yayımlanma Tarihi

3 Mart 2019

Gönderilme Tarihi

31 Mayıs 2018

Kabul Tarihi

16 Ekim 2018

Yayımlandığı Sayı

Yıl 2019 Cilt: 46 Sayı: 1

DOI

https://doi.org/10.5798/dicletip.534825

IZ

https://izlik.org/JA47ED85KA

Kaynak Göster

RIS / Bibtex

APA

Gedikli, S., & Şengül, E. (2019). Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri. Dicle Medical Journal, 46(1), 41-50. https://doi.org/10.5798/dicletip.534825

AMA

1.Gedikli S, Şengül E. Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri. diclemedj. 2019;46(1):41-50. doi:10.5798/dicletip.534825

Chicago

Gedikli, Semin, ve Emin Şengül. 2019. “Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri”. Dicle Medical Journal 46 (1): 41-50. https://doi.org/10.5798/dicletip.534825.

EndNote

Gedikli S, Şengül E (01 Mart 2019) Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri. Dicle Medical Journal 46 1 41–50.

IEEE

[1]S. Gedikli ve E. Şengül, “Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri”, diclemedj, c. 46, sy 1, ss. 41–50, Mar. 2019, doi: 10.5798/dicletip.534825.

ISNAD

Gedikli, Semin - Şengül, Emin. “Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri”. Dicle Medical Journal 46/1 (01 Mart 2019): 41-50. https://doi.org/10.5798/dicletip.534825.

JAMA

1.Gedikli S, Şengül E. Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri. diclemedj. 2019;46:41–50.

MLA

Gedikli, Semin, ve Emin Şengül. “Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri”. Dicle Medical Journal, c. 46, sy 1, Mart 2019, ss. 41-50, doi:10.5798/dicletip.534825.

Vancouver

1.Semin Gedikli, Emin Şengül. Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri. diclemedj. 01 Mart 2019;46(1):41-50. doi:10.5798/dicletip.534825

Cited By

Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats

Atatürk Üniversitesi Veteriner Bilimleri Dergisi

https://doi.org/10.17094/ataunivbd.870668

Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri

Ratlarda Siklofosfamid ile İndüklenen Hepatotoksisite Üzerine Kuersetinin Etkileri

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats

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