Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens

Hamdi Tasli; Burcu Eser; Hakan Birkent; Burak Asik; Mustafa Gerek

doi:10.5798/dicletip.661237

Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens

Öz

Objective: Squamous cell carcinoma (SCC) composes 95% of the carcinoma of the larynx. One of the factor recognized in the past two decades as increasing the risk of SCC is laryngopharyngeal reflux (LPR). The role and mechanism of LPR in laryngeal cancer remain unclear. The aim of this study was to assess the presence of pepsin in laryngeal formalin-fixed, paraffin-embedded (FFPE) squamous cell carcinoma (SCC) specimens in order to test the hypothesis that LPR plays a role in the development of laryngeal SCC. Method: A total of 29 pathology specimens of advanced-stage laryngeal cancer patients who had undergone total laryngectomy were evaluated. The specimens were confirmed histopathologically as SCC and maintained in paraffin blocks in the pathology department. The western blot (WB) or the enzyme-linked immunosorbent assay (ELISA) analyses were used to measure pepsin enzyme levels. Results: According to the WB and ELISA analyses, there was no presence of the pepsin enzyme in any of the 29 formalin-fixed, paraffin-embedded (FFPE) laryngeal SCC specimens. However, pepsin was detected with both the WB and the ELISA tests in positive controls and fresh rat stomach specimen. Conclusion: Pepsin was not present in any of the malignant laryngeal squamous cell carcinoma specimens in this study. Relationship between LPR and laryngeal SCC still remains unclear and further studies are needed to verify the role of pepsin and LPR in laryngeal SCC.

Anahtar Kelimeler

Laryngopharyngeal reflux; laryngeal carcinoma; pepsin; larynx

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Hamdi Tasli ^* Bu kişi benim
0000-0003-2186-5006
Türkiye

Burcu Eser Bu kişi benim
0000-0001-5641-3734
Türkiye

Hakan Birkent Bu kişi benim
0000-0001-9545-6518
Türkiye

Burak Asik Bu kişi benim
Türkiye

Mustafa Gerek Bu kişi benim
Türkiye

Yayımlanma Tarihi

15 Aralık 2019

Gönderilme Tarihi

3 Nisan 2019

Kabul Tarihi

23 Eylül 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 46 Sayı: 4

DOI

https://doi.org/10.5798/dicletip.661237

IZ

https://izlik.org/JA72GF96MG

Kaynak Göster

RIS / Bibtex

APA

Tasli, H., Eser, B., Birkent, H., Asik, B., & Gerek, M. (2019). Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. Dicle Medical Journal, 46(4), 657-665. https://doi.org/10.5798/dicletip.661237

AMA

1.Tasli H, Eser B, Birkent H, Asik B, Gerek M. Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. diclemedj. 2019;46(4):657-665. doi:10.5798/dicletip.661237

Chicago

Tasli, Hamdi, Burcu Eser, Hakan Birkent, Burak Asik, ve Mustafa Gerek. 2019. “Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens”. Dicle Medical Journal 46 (4): 657-65. https://doi.org/10.5798/dicletip.661237.

EndNote

Tasli H, Eser B, Birkent H, Asik B, Gerek M (01 Aralık 2019) Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. Dicle Medical Journal 46 4 657–665.

IEEE

[1]H. Tasli, B. Eser, H. Birkent, B. Asik, ve M. Gerek, “Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens”, diclemedj, c. 46, sy 4, ss. 657–665, Ara. 2019, doi: 10.5798/dicletip.661237.

ISNAD

Tasli, Hamdi - Eser, Burcu - Birkent, Hakan - Asik, Burak - Gerek, Mustafa. “Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens”. Dicle Medical Journal 46/4 (01 Aralık 2019): 657-665. https://doi.org/10.5798/dicletip.661237.

JAMA

1.Tasli H, Eser B, Birkent H, Asik B, Gerek M. Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. diclemedj. 2019;46:657–665.

MLA

Tasli, Hamdi, vd. “Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens”. Dicle Medical Journal, c. 46, sy 4, Aralık 2019, ss. 657-65, doi:10.5798/dicletip.661237.

Vancouver

1.Hamdi Tasli, Burcu Eser, Hakan Birkent, Burak Asik, Mustafa Gerek. Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. diclemedj. 01 Aralık 2019;46(4):657-65. doi:10.5798/dicletip.661237