Determination of Antioxidant Capacity, Total Phenolic Content and Phenolic Acid Profiles of Some Wild Fruit

Abstract

In this study, it was aimed to determine the phenolic substance profiles and antioxidant activities of wild fruit species such as, wild pear, hawthorn, cornelian cherry, firethorn which grow naturally in Turkey. For this purpose, the samples dried in the drying oven has been used in extraction. Total phenolic content was determined by FCR method, antioxidant capacity was determined by DPPH, CUPRAC, ABTS and β-carotene bleaching methods. The phenolic profiles of the fruits were illuminated by HPLC. Total phenolic content of the fruits was determined as 493 (wild pear), 847 (hawtorn), 823 (cornelian cherry), 2555 (firethorn) mg GAE/100 g. The results of antioxidant capacity of the wild pear, hawtorn, cornelian cherry and firethorn were determined as 12.275, 22.754, 47.705, 83.134 %TAC in DPPH method, 0.258, 0.619, 0.684, 2.871 mmol TE/g in CUPRAC, 73.78, 49.33, 51.56, 79.34 % TEAC in ABTS and 93.63%, 96.67%, 88.70%, 97.18% in β-carotene bleaching method, in respect. The fruits mainly contain chlorogenic acid, caffeic acid, vanillic acid and synapic acid.

Keywords

• Wild fruit
• phenolic acid profile
• antioxidant
• total phenolic content

Bazı Yabani Meyve Türlerinin Antioksidan Kapasitesi, Toplam Fenolik Madde İçeriği ve Fenolik Asit Profilinin Belirlenmesi

Öz

Bu çalışmada ülkemizde doğal olarak yetişen ahlat, alıç, kızılcık ve ateş dikeni meyvelerinin fenolik madde profilleri ve antioksidan kapasitelerinin belirlenmesi amaçlanmıştır. Meyve örnekleri etüv yardımıyla kurutularak ekstrakte edilmiştir. Sonrasında ise toplam fenolik madde içeriği Folin Ciocalteu (FCR) ayıracı ile antioksidan kapasiteleri ise DPPH, CUPRAC, ABTS ve β-karoten ağartma yöntemleri kullanılarak yapılmıştır. Meyvelerin fenolik madde profilleri ise yüksek performanslı sıvı kromatografisi (HPLC) yoluyla 9 fenolik asit standardı kullanılarak belirlenmiştir. Ahlat, alıç, kızılcık ve ateş dikeni meyvelerinin toplam fenolik madde içerikleri sırası ile 493, 847, 823, 2555 mg GAE/100g olarak belirlenmiştir. İncelenen numunelerin DPPH yöntemine göre antioksidan kapasiteleri sırası ile %12.275, %22.754, %47.705, %83.134; CUPRAC yöntemine göre antioksidan kapasiteleri 0.258, 0.619, 0.684, 2.871 mmol TE/g; ABTS yöntemine göre antioksidan kapasiteleri 73.78, 49.33, 51.56, 79.34 %TEAC ve β-karoten ağartma metoduna göre %93.63, %96.67, %88.70, %97.18 olarak saptanmıştır. HPLC kullanılarak elde edilen fenolik profil sonuçlarına göre incelenen yabani meyvelerde klorojenik asit, kafeik asit, vanilik asit ve sinapik asit bileşenlerinin ağırlıklı olduğu tespit edilmiştir.

Anahtar Kelimeler

• Yabani meyve
• antioksidan
• fenolik asit profili
• toplam fenolik içerik

Kaynakça

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