Yıl 2019, Cilt , Sayı 16, Sayfalar 933 - 939 2019-08-31

Antioxidant Activity of Uvez Fruit
Üvez Meyvesinin Antioksidan Aktivitesi

Yüksel Bayram [1] , Yasemin Torlak [2] , Osman Sağdıç [3]


For centuries, a variety of medicinally important fruits have been used in all countries to treat diseases in public health. Among these fruits, the antioxidant activity of rowan fruit has recently attracted attention. Rowan fruit (Sorbus aucuparia) of naturally grown plants in Turkey are determined by studies that contain important bioactive components in terms of human health. It is important to investigate the potential use of this fruit which is widely used in jam making in health and food sectors. The aim of this study was to investigate the total phenolic and flavonoid contents and antioxidant activities of rowan fruit extracts obtained under different extraction conditions.

In this study, rowan fruits collected from Çal region (Denizli, Turkey) were grinded after drying and extracts were obtained in three different solvents by ultrasound assisted extraction method. Ethanol:water (1:1), methanol:water (1:1), acetone:water (1:1) solutions were used for extraction and extraction was carried out for 40 minutes in an ultrasonic water bath at 50 ± 5°C for each solvent separately and bioactive values were examined. The extract yield, total phenolic and flavonoid substance and antioxidant activity in DPPH method were determined according to the working conditions of each extract. When comparisons are made with tables and figures, the best result is the ethanol:water mixture.

Yüzyıllardan beri, bütün ülkelerde tıbbi açıdan önemli olan çeşitli meyveler, halk sağlığında hastalıkların tedavisi amacıyla kullanılmaktadır. Bu meyveler arasında üvez meyvesinin son zamanlarda antioksidan aktivitesi dikkat çekmektedir. Türkiye‘de doğal olarak yetişen Kuş üvezi (Sorbus aucuparia L) ‘nin insan sağlığı açısından önemli biyoaktif bileşenler içerdiği yapılan çalışmalarla belirlenmiştir. Yaygın olarak reçel yapımında değerlendirilen bu meyvenin sağlık ve gıda sektörlerinde kullanım potansiyelinin araştırılması önem arz etmektedir.

Bu çalışmanın amacı farklı ekstraksiyon koşullarında elde edilen üvez meyvesi ekstraktlarının, toplam fenolik ve flavonoid madde miktarları ile antioksidan aktivitelerinin incelenmesidir. Çalışmada Çal‘dan (Denizli) toplanan üvez meyveleri kurutulduktan sonra öğütülmüş ve ultrason destekli ekstraksiyon yöntemi ile üç farklı çözücü içerisinde ekstraktlar elde edilmiştir. Ekstraksiyon işlemi için etanol:su (1:1), methanol:su (1:1), aseton:su (1:1) çözeltileri kullanılmış ve her solvent için ayrı ayrı ultrasonik su banyosunda 50±5 oC sıcaklıkta 40 dakika süreyle ekstraksiyon işlemleri gerçekleştirilmiş ve biyoaktif değerleri incelenmiştir. Çalışmada, ekstrakt verimi, toplam fenolik ve flavonoid madde ile antioksidan aktivite (DPPH (2,2-difenil-1-pikril hidrazil))   değerlerinin en iyi çıktığı çalışma koşulları, herbir ekstrakt elde etme yöntemine göre açıklanmış ve tablo ve şekillerle karşılaştırmalar yapılarak en iyi sonucun etanol:su karışımına (1:1) ait olduğu görülmüştür.

  • Balasundram, N., Sundram, K., & Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry. https://doi.org/10.1016/j.foodchem.2005.07.042.
  • Bondet, V., Brand-Williams, W., & Berset, C. (1997). Kinetics and Mechanisms of Antioxidant Activity using the DPPH.Free Radical Method. LWT - Food Science and Technology, 30(6), 609–615. https://doi.org/10.1006/fstl.1997.0240.
  • Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology. https://doi.org/10.1016/S0023-6438(95)80008-5.
  • Chen, Z., Bertin, R., & Froldi, G. (2013). EC50 estimation of antioxidant activity in DPPH* assay using several statistical programs. Food Chemistry. https://doi.org/10.1016/j.foodchem.2012.11.001.
  • Dai, J., & Mumper, R. J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules. doi.org/10.3390/molecules15107313.
  • Del Rio, D., Borges, G., & Crozier, A. (2010). Berry flavonoids and phenolics: Bioavailability and evidence of protective effects. British Journal of Nutrition. https://doi.org/10.1017/S0007114510003958.
  • Generalic, I., Skroza, D., Ljubenkov, I., Katalinic, A., Burcul, F., Katalinic, V. (2011). Influence of the phenophase on the phenolic profile and antioxidant proterties of Dalmatian sage. Food Chemistry, 127, 427- 433.
  • Gil-Izquierdo, A., & Mellenthin, A. (2001). Identification and quantitation of flavonols in rowanberry (Sorbus aucuparia L.) juice. European Food Research and Technology. https://doi.org/10.1007/s002170100328.
  • Isaikina, N. V., Kalinkina, G. I., Razina, T. G., Zueva, E. P., Rybalkina, O. Y., Ulirich, A. V., … Shilova, A. B. (2018). Sorbus aucuparia L. Fruit Is a Source of the Drug for Increasing the Efficiency of Tumor Chemotherapy. Russian Journal of Bioorganic Chemistry. https://doi.org/10.1134/s1068162018070038.
  • Kähkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J. P., Pihlaja, K., Kujala, T. S., & Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf990146l.
  • Korkut; M. Budakci. (2010). The effects of high-temperature heat-treatment on physical properties and surface roughness of rowan (Sorbus aucuparial.) wood. Wood Research.
  • Korkut, S., Guller, B., Aytin, A., & Kok, M. S. (2009). Turkey’s Native Wood Species: Physical and Mechanical Characterization and Surface Roughness of Rowan (Sorbus Aucuparia L.). Wood Research.
  • Kumaran, A., & Joel Karunakaran, R. (2007). In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT - Food Science and Technology. https://doi.org/10.1016/j.lwt.2005.09.011.
  • Lang, Q., & Wai, C. M. (2001). Supercritical fluid extraction in herbal and natural product studies - A practical review. Talanta. https://doi.org/10.1016/S0039-9140(00)00557-9.
  • Lehotay, S. J. (1997). Supercritical fluid extraction of pesticides in foods. Journal of Chromatography A. https://doi.org/10.1016/S0021-9673(97)00461-5.
  • Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., & Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry. https://doi.org/10.1016/j.foodchem.2005.02.033.
  • Lüle, F., & Koyuncu, T. (2015). Convective and Microwave Drying Characteristics of Sorbus Fruits (Sorbus domestica L.). Procedia - Social and Behavioral Sciences. https://doi.org/10.1016/j.sbspro.2015.06.467.
  • Martins, N., Barros, L., Buelga, C., Henriques, M., Silva, S. Ve Ferreiara I. (2015). Evaluation of bioactive properties and phenolic compounds in different extracts prepared from Salvia officinalis L..Food Chemistry, 170: 378-385.
  • Mikulic-Petkovsek, M., Schmitzer, V., Slatnar, A., Stampar, F., & Veberic, R. (2012). Composition of Sugars, Organic Acids, and Total Phenolics in 25 Wild or Cultivated Berry Species. Journal of Food Science. https://doi.org/10.1111/j.1750-3841.2012.02896.x.
  • Pérez-Jiménez, J. Ve Saura-Calixto, F. (2006). Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Research International, 39(7):791-800.
  • Razina, T. G., Zueva, E. P., Ulrich, A. V., Rybalkina, O. Y., Chaikovskii, A. V., Isaikina, N. V., … Zyuz’Kov, G. N. (2016). Antitumor effects of sorbus aucuparia L. Extract highly saturated with anthocyans and their mechanisms. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-016-3554-4.
  • Roby, M. H. H., Sarhan, M. A., Selim, K. A., Khalel, K. I. (2013). Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.) and marjoram (Origanum majorana L.) extracts. Industrial Crops and Products, 43, 827-831.
  • Salem, I. B., Fekih, S., Sghaier, H., Bousselmi, M., Saidi, M., Landoulsi, A., Fattouch, S. (2013). Effect of ionising radiation on polyphenolic content and antioxidant potential of parathon- treated sage (Salvia officinalis) leaves. Food Chemistry, 141, 1398-1405.
  • Sánchez-Moreno, C. (2002). Methods Used to Evaluate the Free Radical Scavenging Activity in Foods and Biological Systems. Food Science and Technology International. https://doi.org/10.1106/108201302026770.
  • Šavikin, K. P., Zdunić, G. M., Krstić-Milošević, D. B., Šircelj, H. J., Stešević, D. D., & Pljevljakušić, D. S. (2017). Sorbus aucuparia and Sorbus aria as a Source of Antioxidant Phenolics, Tocopherols, and Pigments. Chemistry and Biodiversity. https://doi.org/10.1002/cbdv.20170032.9.
  • Singleton, V. L., & Rossi, J. A. J. (1965). Colorometry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. https://doi.org/10.12691/ijebb-2-1-5.
  • Stalikas, C. D. (2007). Extraction, separation, and detection methods for phenolic acids and flavonoids. Journal of Separation Science. https://doi.org/10.1002/jssc.200700261.Tian, Y., Puganen, A., Alakomi, H. L., Uusitupa, A., Saarela, M., & Yang, B. (2018). Antioxidative and antibacterial activities of aqueous ethanol extracts of berries, leaves, and branches of berry plants. Food Research International. https://doi.org/10.1016/j.foodres.2017.12.071.
  • Velioglu, Y. S., & Mazza, G. (1991). Characterization of Flavonoids in Petals of Rosa damascena by HPLC and Spectral Analysis. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf00003a007.
  • Vernon, L. S., Rudolf, O., & Rosa, M. L.-R. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology.
  • Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. https://doi.org/10.1016/S0308-8146(98)00102-2.
Birincil Dil tr
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Orcid: 0000-0002-1130-577X
Yazar: Yüksel Bayram (Sorumlu Yazar)
Kurum: PAMUKKALE ÜNİVERSİTESİ, ÇAL MESLEK YÜKSEKOKULU
Ülke: Turkey


Orcid: 0000-0001-5964-2532
Yazar: Yasemin Torlak
Kurum: PAMUKKALE ÜNİVERSİTESİ, ÇAL MESLEK YÜKSEKOKULU
Ülke: Turkey


Orcid: 0000-0002-2063-1462
Yazar: Osman Sağdıç
Kurum: YILDIZ TEKNİK ÜNİVERSİTESİ, KİMYA-METALURJİ FAKÜLTESİ
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 31 Ağustos 2019

Bibtex @araştırma makalesi { ejosat589736, journal = {Avrupa Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2683}, address = {}, publisher = {Osman SAĞDIÇ}, year = {2019}, volume = {}, pages = {933 - 939}, doi = {10.31590/ejosat.589736}, title = {Üvez Meyvesinin Antioksidan Aktivitesi}, key = {cite}, author = {Bayram, Yüksel and Torlak, Yasemin and Sağdıç, Osman} }
APA Bayram, Y , Torlak, Y , Sağdıç, O . (2019). Üvez Meyvesinin Antioksidan Aktivitesi. Avrupa Bilim ve Teknoloji Dergisi , (16) , 933-939 . DOI: 10.31590/ejosat.589736
MLA Bayram, Y , Torlak, Y , Sağdıç, O . "Üvez Meyvesinin Antioksidan Aktivitesi". Avrupa Bilim ve Teknoloji Dergisi (2019 ): 933-939 <https://dergipark.org.tr/tr/pub/ejosat/issue/45333/589736>
Chicago Bayram, Y , Torlak, Y , Sağdıç, O . "Üvez Meyvesinin Antioksidan Aktivitesi". Avrupa Bilim ve Teknoloji Dergisi (2019 ): 933-939
RIS TY - JOUR T1 - Üvez Meyvesinin Antioksidan Aktivitesi AU - Yüksel Bayram , Yasemin Torlak , Osman Sağdıç Y1 - 2019 PY - 2019 N1 - doi: 10.31590/ejosat.589736 DO - 10.31590/ejosat.589736 T2 - Avrupa Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 933 EP - 939 VL - IS - 16 SN - -2148-2683 M3 - doi: 10.31590/ejosat.589736 UR - https://doi.org/10.31590/ejosat.589736 Y2 - 2019 ER -
EndNote %0 Avrupa Bilim ve Teknoloji Dergisi Üvez Meyvesinin Antioksidan Aktivitesi %A Yüksel Bayram , Yasemin Torlak , Osman Sağdıç %T Üvez Meyvesinin Antioksidan Aktivitesi %D 2019 %J Avrupa Bilim ve Teknoloji Dergisi %P -2148-2683 %V %N 16 %R doi: 10.31590/ejosat.589736 %U 10.31590/ejosat.589736
ISNAD Bayram, Yüksel , Torlak, Yasemin , Sağdıç, Osman . "Üvez Meyvesinin Antioksidan Aktivitesi". Avrupa Bilim ve Teknoloji Dergisi / 16 (Ağustos 2019): 933-939 . https://doi.org/10.31590/ejosat.589736
AMA Bayram Y , Torlak Y , Sağdıç O . Üvez Meyvesinin Antioksidan Aktivitesi. Avrupa Bilim ve Teknoloji Dergisi. 2019; (16): 933-939.
Vancouver Bayram Y , Torlak Y , Sağdıç O . Üvez Meyvesinin Antioksidan Aktivitesi. Avrupa Bilim ve Teknoloji Dergisi. 2019; (16): 939-933.