Rekombinant ve Yabanıl Bakteriler ile Kromun Biyoremediasyonu

Yıl 2020, Ejosat Özel Sayı 2020 (ARACONF), 604 - 609, 01.04.2020

Şeyma Akkurt Merve Oğuz

https://doi.org/10.31590/ejosat.araconf73

Öz

Günümüzde ağır metal kirliliği önemli çevre sorunlarından biri haline gelmiştir. Kadmiyum, kurşun ve krom gibi ağır metallerin düşük konsantrasyonları canlılar için toksiktir. Krom (Cr), dünyadaki en toksik ağır metallerden biridir ve endüstriyel olarak salınan yaygın bir çevresel kirleticilerdendir. Cr metal iyonu; biyolojik olarak parçalanmayan ve doğada biriken bir ağır metaldir. Krom, biyolojik olarak gerekli değildir ve alıcı ortamlara (su ve toprak gibi) yapılan deşarjlar sonucunda besin zinciri boyunca birikerek insanlara kadar aktarılır. Kromun ana kaynakları; tabakhaneler, elektrokaplama, madencilik, tekstil, metal işleme, gübre, boyalar ve pigment imalat sanayi gibi çeşitli endüstrilerdir. İnsan sağlığı ve su ekosistemleri üzerinde olumsuz etkileri olan krom metal iyonları, farklı arıtma teknolojileri ile su ve atık sudan uzaklaştırılmalıdır.
Krom giderimin de yaygın kullanılan metotlar; ters osmoz, elektrokimyasal prosesler, iyon değişimi, aktif karbona adsorpsiyon ve katılaştırma/stabilizasyondur. Bu yöntemlerin pahalı olmaları, yan ürün oluşumu ve çevre dostu olmamaları gibi bazı dezavantajları vardır. Biyoremediasyon prosesleri ise tüm bu sorunlara bir çözüm getirmiş ve çevre, ekonomi ve enerji açısından fayda sağlamıştır.
Bakterilerin ağır metallere karşı geliştirdikleri direnç mekanizmaları sayesinde, ağır metallerin yüksek derişimlerinin olduğu ortamlarda hayatta kalırlar. Bakterilerin krom metal iyonunu bağlama kapasitelerini artırmak için biyoremediasyon umut verici bir prosestir. Yabanıl bakterilere, metalotiyonein, poli-histidinler veya poli-sisteinler gibi peptidler eksprese edilerek metal bağlama kapasitesini artırılabilmektedir. Bu derlemede; hücre içine metal girişinin engellenmesi, metalin proteinlere bağlanarak hücre içinde tutulması, metallerin hücrenin dışında tutulması, enzimlerle metalin daha az toksik forma dönüştürülmesi gibi direnç mekanizmalarına yer verilmiştir.
Bu çalışmada, bazı yabanıl ve rekombinant bakteri türlerinin krom metal iyonunu giderimleri araştırılmıştır. Bu derlemenin amacı kromu etkili bir şekilde bağlayan bakterileri ve proteinleri özetlemektir.

Anahtar Kelimeler

Ağır metal, biyoremediasyon, krom

Bioremediation of Chromium with Recombinant and Wild Bacteria

Öz

Today, heavy metal pollution has become one of the most important environmental problems. Low concentrations of heavy metals such as cadmium, lead and chromium are toxic to living things. Chromium (Cr) is one of the most toxic heavy metals in the world and is a common industrially released environmental pollutant. Chromium is a heavy metal that is non-biodegradable and accumulated in nature. For this reason, it is very important to treat chromium metal ion by making it suitable for discharge limits. Chromium is not biologically necessary and accumulates along the food chain as a result of discharges to receiving environments (such as water and soil) and transferred to humans. The main sources of chromium are various industries such as tanneries, electroplating, mining, textile, metal processing, fertilizer, dyes and pigment manufacturing industry. Chromium metal ions, which has negative effects on human health and water ecosystems, should be removed from water and wastewater with different treatment technologies.
The most widely used methods are for chromium removal conventional physicochemical processes such as reverse osmosis, electrochemical process, ion exchange, adsorption on activated carbon, and solidification/stabilization, etc. These disadvantages, such as being expensive, waste product formation and being not environmentally friendly, limit their use. Bioremediation processes have brought a solution to all these problems and have benefited in terms of environment, economy and energy. Thanks to the resistance mechanisms developed by bacterias against heavy metals, they survive in environments with high concentrations of heavy metals. Bioremediation is a promising process to increase the capacity of bacteria to bind chromium metal ion. In this review; Resistance mechanisms such as preventing the entry of metal into the cell, keeping the metal in the cell by binding to proteins, keeping the metals out of the cell, and turning the metal into less toxic form with enzymes are included.
In this study, the efficiencies of some wild and recombinant bacterial species on the removal of chromium will be investigated. The purpose of this review is to summarize bacteria and proteins that bind chromium metal ions effectively.

Anahtar Kelimeler

Heavy metal, bioremediation, chromium

Kaynakça

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