Arbutin’in yeni kaynakları: Onobrychis nitida and Onobrychis galegifolia

Yıl 2019, Cilt: 12 Sayı: 3, 1733 - 1737, 31.12.2019

Hüseyin Akşit , Samed Şimşek , Volkan Özdokur , Ekrem Köksal

https://doi.org/10.18185/erzifbed.658444

Cited By: 1

Öz

Arbutin (4-Hidroksifenil-β-D-glukopiranozit),
hidrokinonun mono glikozidik formudur ve cilt beyazlatma ve çil giderme
özellikleri nedeniyle kozmetiklerde uzun süredir yaygın olarak
kullanılmaktadır. Bu çalışmada; arbutin flash kromatografi kullanılarak Onobrychis
galegifolia'nın metanol ekstraktından izole edilmiştir. Arbutin yapısı, ¹H
ve ¹³C -NMR spektroskopisi ve ayrıca 2D NMR teknikleri ile
aydınlatıldı. Ek olarak, O. galegifolia ve O. nitida türlerinde
bulunan arbutinin kantitatif analizi HPLC-DAD kullanılarak yapıldı. Sonuçlara
göre, O. galegifolia ve O. nitida'nın metanol ekstraktlarının
arbutin içeriği sırasıyla 212.02 ve 196.46 mg/g ekstrakt olarak bulundu.
Ayrıca, O. galegifolia ve O. nitida'nın soğuk su ekstraktlarının
arbutin içeriği 128.2 ve 99.40 mg/g ekstrakt olarak bulundu. Kuru
ağırlıklardaki arbutin miktarı, yaklaşık% 5.66 ila% 6.86 arasında hesaplandı.

Anahtar Kelimeler

Onobrychis nitida, Arbutin

New sources of arbutin: Onobrychis nitida and Onobrychis galegifolia

Öz

Arbutin
(4-Hydroxyphenyl-β-D-glucopyranoside) is a mono glycosidic form of
hydroquinone and has been widely used in cosmetics for a long time due to skin
whitening and freckle removing properties. In this study, arbutin was isolated
from the methanol extract of Onobrychis galegifolia using flash
chromatography. The structure of arbutin was elucidated by ¹H and ¹³C
-NMR spectroscopy and as well as 2D NMR techniques. In addition, quantitative
analysis of arbutin found in O. galegifolia and O. nitida species
was done using HPLC-DAD. According to the results, the arbutin content of
methanol extracts of O. galegifolia and O. nitida was found
212.02 and 196.46 mg/g extract respectively. In addition, the arbutin content
of cold-water extracts of O. galegifolia and O. nitida was found
128.2 and 99.40 mg/g extract. The amount of arbutin in dry weights was
calculated for about 5.66% to 6.86%.

Anahtar Kelimeler

Arbutin, Onobrychis nitida

Kaynakça

• Bol', M. Y., et al. (1976). "Polyphenols of Onobrychis vassiltschenkoi and their polarographic determination." Chemistry of Natural Compounds 12(1): 91-92.
• Kandemir, A. (2009). "The rediscovery of some taxa thought to have been extinct in Turkey." Turkish Journal of Botany 33(2): 113-122.
• Kandemir, A., et al. (2018). "Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants."
• Luk'yanchikov, M. (1982). "Polyphenols of Onobrychis bobrovii." Chemistry of Natural Compounds 18(2): 237-237.
• Marais, J. P., et al. (2000). "Polyphenols, condensed tannins, and other natural products in Onobrychis viciifolia (sainfoin)." Journal of Agricultural and Food Chemistry 48(8): 3440-3447.
• Moniava, I. (1970). "Arbutin from Onobrychis kachetica." Chemistry of Natural Compounds 6(2): 270-270.
• Regos, I. and D. Treutter (2010). "Optimization of a high-performance liquid chromatography method for the analysis of complex polyphenol mixtures and application for sainfoin extracts (Onobrychis viciifolia)." Journal of Chromatography A 1217(40): 6169-6177.
• Regos, I., et al. (2009). "Identification and quantification of phenolic compounds from the forage legume sainfoin (Onobrychis viciifolia)." Journal of Agricultural and Food Chemistry 57(13): 5843-5852.