ATLARDA EGZERSİZİN NİTRİK OKSİT, İNTERLÖYKİN-10 DÜZEYLERİ VE KASPAZ-6 AKTİVİTESİ İLE BAZI BİYOKİMYASAL PARAMETRELERE ETKİSİ

Öz

Bu çalışmada, 20 adet Safkan İngiliz atlarında egzersizin bazı biyokimyasal parametrelerin yanında oksidatif stres göstergelerinden nitrik oksit (NO), temel inflamatuvar sitokinlerden interlöykin-10 (IL-10) düzeyleri ve apoptoz belirteçlerinden kaspaz-6 aktivitesi üzerindeki etkileri belirlendi. Bu amaçla atlar, 10 günlük süre boyunca 11:00 ve 13:00 saatleri arasında günde bir kez egzersize tabi tutuldu. Egzersiz öncesi ve sonrası hayvanların v. jugularisinden kanları alınıp, elde edilen serumlarda aspartat amino transferaz (AST), laktat dehidrojenaz (LDH), kreatin kinaz (CK) enzim aktiviteleri ile glikoz, toplam kolesterol, trigliserid, albümin, üre ve kreatinin, plazmalarda NO, IL-10 düzeyleri ile kaspaz-6 aktiviteleri belirlendi. Egzersiz; serum AST, LDH, CK, plazma kaspaz-6 aktiviteleri ile IL-10 ve serum albümin düzeylerini etkilemedi. Bununla birlikte egzersize bağlı olarak, her ne kadar referans değerler arasında olmakla birlikte, serum glikoz düzeylerinde düşme, toplam kolesterol, trigliserid, üre, kreatinin ve plazma NO düzeylerinde ise yükselme saptandı. Sonuç olarak; yarış atlarında incelenen biyokimyasal parametreler, oksidatif stres, sitokin ve apoptoz belirteçlerinin egzersiz süresi, türü ve yoğunluğuna bağlı olarak değişebileceği kanaatine varıldı.

Anahtar Kelimeler

• At
• egzersiz
• interlöykin-10
• kaspaz-6
• nitrik oksit

EFFECT OF EXERCISE ON NITRIC OXIDE, INTERLEUKIN-10 LEVELS, CASPASE-6 ACTIVITY AND SOME BIOCHEMICAL PARAMETERS IN HORSES

Öz

In this study, the effects of exercise on some biochemical parameters as well as nitric oxide (NO), one of the oxidative stress indicators, interleukin-10 (IL-10), one of the main inflammatory cytokines, and caspase-6 activity, one of the apoptosis markers, were determined in 20 Thoroughbred horses. For this purpose, horses were exercised once a day between 11:00 and 13:00for 10 days. Blood samples were taken from the v. jugularis of the animals before and after exercise, and in the serum obtained aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK) enzyme activities, glucose, total cholesterol, triglyceride, albumin urea, and creatinine, in plasma NO, IL-10 levels and caspase-6 activities were determined. Exercise did not affect serum AST, LDH, CK, plasma caspase-6 activities, IL-10, and serum albumin levels. However, depending on the exercise; although it is between reference values; a decrease in serum glucose levels, an increase in total cholesterol, triglyceride, urea, creatinine, and plasma NO levels were detected. As a result; it was concluded that the biochemical parameters, oxidative stress, cytokine, and apoptosis markers examined in racehorses may vary depending on the duration, type, and intensity of exercise.

Anahtar Kelimeler

• Caspase-6
• exercise
• horse
• inlerleukin-10
• nitric oxide

Kaynakça

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