Clitocybe geotropa TÜRÜ MANTARDAN ELDE EDİLEN EKSTRAKTLARIN MDA-MB-231 HÜCRE HATTI ÜZERİNDEKİ SİTOTOKSİK AKTİVİTESİNİN İNCELENMESİ

Öz

Genel Bilgi: Mantarların tıbbi amaçlı olarak kullanımlarının geçmişi Asya bölgesi için oldukça eskiye dayanmaktadır ve son zamanlarda Batı’da kullanımları Kabul görmektedir. Makro mantarlardan izole edilen bileşenlerin veya ekstrelerin antimikrobiyal, anti-viral, antitümör, anti-alerjik, immünomodülatör, anti- enflamatuar, anti-aterojenik, hipoglisemik ve hepatoprotektif etki gibi çeşitli biyolojik aktivitelerinin olduğu bildirilmiştir. Amaç: Bu çalışmada, Clitocybe geotropa (Bull.) Quél.’den elde edilen etanol ve metanol ekstrelerinin meme kanseri hücreleri üzerine etkinliği araştırılmıştır. Yöntem: Bu çalışmada mantar ekstrelerinin verimi, belli ağırlıktaki kuru materyalden elde edilen etanol ekstresinin (% 28,2) ve metanol ekstresinin (% 30,6) ağırlıklarına göre g/100g olarak ifade edilerek belirlenmiştir. Ekstrelerin sitotoksik etkileri Trypan Blue analiziyle belirlenmiştir. Bulgular: Etanol ve metanol ekstrelerinin total fenolik içeriği sırasıyla 217,15±0,02 ve 138,39±0,01; total flavanoit içeriği 152,28±0,03 ve 111,95±0,02 mgCE/g olarak ölçülmüştür. C. geotropa için 50–400 µg/mL konsantrasyon değerlerindeki metanol ekstrelerinin MDA-MB-231 hücre hattı üzerinde sitotoksik etkisi gözlenmezken, etanol ekstresi % 51,5; 400 μg/ml, % 17,9; 200 μg/ml, % 6,6; 100 μg/ml uygulanan konsantrasyonlarda anlamlı düzeyde sitotoksik etki göstermiştir. Sonuç: Clitocybe geotropa MDA-MB-231 hücre hattı üzerinde etkili antikanser ajan olarak kullanılma potansiyeline sahip bir mantar türüdür.

Anahtar Kelimeler

• C. geotropa, toplam fenolik, toplam flavanoit, sitotoksite

Cytotoxic Activity of Two Extracts from the Mushroom Clitocybe Geotropa in Mda-Mb-231Cell Line

Öz

Background: The medical use of mushrooms has a very long tradition in Asia and is nowadays becoming more accepted in the West. It is reported that the extracts or isolated constituents from macrofungi have biological activities such as antimicrobial, anti-viral, anti-tumour, anti-allergic, immunomodulating, anti-inflammatory, antiatherogenic, hypoglycaemic and hepatoprotective activities. Objective: In this study, cytotoxic activity of ethanol extracts (EE) and methanol (ME) of Clitocybe geotropa on MDA-MB-231 cell line were investigated. Method: The yield of mushroom extracts was calculated for EE and ME 30.6% and 28.2%, respectively. Cytotoxic effects of the extracts were determined by Trypan Blue assay. Results: The total phenolic contents of EE and ME were 217.15±0.02, 138.39±0.01; total flavonoid contents 152.28±0.03, 111.95±0.02 mgCE/g, respectively. The ME of mushroom’s 50–400 µg/mL concentrations did not show any cytotoxic effect in MDA-MB-231 cell line while the EE of C. geotropa % 51,5; 400 μg/ml, % 17,9; 200 μg/ml, % 6,6; 100 μg/ml had cytotoxicity. Conclusion: The mushroom, Clitocybe geotropa, could be used as a potential anticancer agent for MDA-MB- 231 cell line.

Anahtar Kelimeler

• C. geotropa, total phenolic, total flavonoid, cytotoxicity

Kaynakça

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