AVEMAR HT29 KOLON KANSERİ HÜCRELERİNDEKİ ANTİ-ANJİYOJENİK ETKİSİNİ COX-2 VE VEGF EKSPRESYONLARINI İNHİBE EDEREK GÖSTERİR

Öz

Fermente buğday tohumu özütünden elde edilen Avemar, tamamen doğal, klinikte özellikle kemoterapi ve radyoterapi tedavisi gören kanser hastalarında diyet desteği olarak kullanılan non-toksik bir bileşiktir. Çalışmada, birçok biyolojik aktiviteye sahip olan bu ekstraktın insan kolon kanseri hücrelerinde anti-anjiyojenik özelliğinin olup olmadığının belirlenmesi amaçlandı. Bu amaçla, Avemar 800, 1600 ve 3200 µg/ml dozlarında HT-29 hücrelerine uygulandı ve sonrasında, en önemli anjiyogenez belirteçlerinden olan VEGF (Vasküler Endotel Büyüme Faktörü) ve Cox-2 (Siklooksijenaz-2) protein ve mRNA miktarlarındaki değişiklikler araştırıldı. Protein miktarlarındaki değişiklikler Elisa yöntemi ile, mRNA düzeylerindeki değişiklikler ise gerçek zamanlı polimeraz zincir reaksiyonu ile belirlendi. Avemar uygulanan hücrelerdeki VEGF ve Cox-2 protein ve mRNA düzeyleri, uygulanmayan HT-29 hücrelerine göre önemli derecede azalma gösterdi. Araştırma sonunda elde edilen veriler, Avemar’ın kolon kanseri hücreleri üzerinde anti-anjiyojenik etkisinin olabileceği yönünde yorumlandı ve tıp dünyasına kolon kanserinin anti-anjiyojenik tedavisinde aday bir ekstrakt olarak aktarıldı

Anahtar Kelimeler

• Avemar, anjiyogenez, kolon kanseri, vasküler endotel büyüme faktörü, siklooksijenaz-2

Avemar Exhibits Anti-Angiogenic Effect Mediated By Inhibition of Cox-2 and Vegf Gene Expression on Ht29 Colon Carcinoma Cells

Öz

Avemar, obtained from fermented wheat germ extract, is a completely natural and non-toxic compound which is clinically used in particular as diet support in cancer patients treated with chemotherapy and radiotherapy. The purpose of this study is to determine whether avemar has an anti-angiogenic effects on human colon cancer cells. For this aim, HT29 cells were exposed to 400, 800, 1600 and 3200 µg/ml concentrations of Avemar, subsequently, protein amounts and mRNA levels of VEGF (Vascular Endothelial Growth Factor) and Cox-2 (Cyclooxygenase-2), which are the most important angiogenic markers, were investigated. While the change in the amount of protein was measured by Elisa assay, the alterations at the mRNA levels were determined by real time PCR. Avemar exhibited significantly important decrease in the both of protein and mRNA levels of VEGF and Cox-2 genes on HT29 cells treated with avemar, compared to non-treated cells. The results of this study led us to interpret that Avemar might be an anti- angiogenic effect on colon cancer cells, and Avemar was ssuggested to medical community as a candidate extract for the therapy of colon cancer.

Anahtar Kelimeler

• Avemar, Angiogenesis, Colon cancer, Vascular endothelial growth factor, Cyclooxygenase-2.

Kaynakça

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