DİMETOAT’IN SIÇAN AKCİĞERİNE OLAN HİSTOPATOLOJİK ETKİSİ VE LAUROCERASUS OFFICINALIS ROEM. (KARAYEMİŞ)MEYVESİNİN KORUYUCU ROLÜ

Öz

Dimethoate is one of the most important and widely used organophosphate insecticides on a large number of crops against several pests. The aim of this study was to evaluate the subchronic toxicity of orally administered dimethoate in Wistar albino rat and the ameliorative effect of cherry laurel fruit extract or vitamin C as control, based on the histopathological findings in the lung tissue. For this purpose, animals were divided randomly into six groups of ten rats each and were treated daily by oral gavage for 60 days. Histopathological alterations in the lung tissue section were determined using a light microscope. Dimethoate exposure exhibited severe histopathological changes in the lung sections compared with control group rats. The morphology of the lung seemed to be mostly affected by dimethoate treatment leading to degenerative changes. An increased hemorrhage within the alveoli, necrosis and alveolar edema as well as an infiltration of inflammatory leukocyte were observed. However, pre- and post-treatment with cherry laurel fruit extract or pretreatment with vitamin C to dimethoate administered rats showed an amelioration in the lung injury. In conclusion, treatment with cherry laurel fruit extract or vitamin C may protect against lung toxicity induced by dimethoate exposure

Anahtar Kelimeler

• Toxicity, dimethoate, lung, histopathology, cherry laurel fruit.

HISTOPATHOLOGICAL EFFECT OF DIMETHOATE ON LUNG OF RAT AND THE PROTECTIVE ROLE OF LAUROCERASUS OFFICINALIS ROEM. (CHERRY LAUREL)FRUIT

Öz

Dimetoat, çok sayıda mahsul üzerinde bulunan birçok zararlıya karşı en önemli ve yaygın kullanılan organofosfatlı insektisitlerden biridir. Bu çalışmanın amacı, Wistar albino sıçanlarına oral olarak uygulanan dimetoatın subkronik toksisitesini ve taflan meyve ekstresinin veya kontrol olarak vitamin C'nin akciğer dokusundaki histopatolojik bulgulara dayalı iyileştirici etkisini değerlendirmektir. Bu amaçla, hayvanlar rastgele her biri on sıçandan oluşan altı gruba ayrıldı ve 60 gün boyunca her gün oral gavaj ile muamele edildi. Akciğer dokusu kesitindeki histopatolojik değişiklikler ışık mikroskop kullanılarak belirlendi. Dimetoat maruziyeti, kontrol grubu sıçanlara kıyasla akciğer bölümlerinde ciddi histopatolojik değişiklikler gösterdi. Akciğerin morfolojisinin çoğunlukla dejeneratif değişikliklere yol açan dimetoat muamelesi ile etkilendiği görüldü. Alveolde artmış kanama, nekroz ve alveolar ödem ile inflamatuvar lökosit infiltrasyonu gözlendi. Bununla birlikte, dimetoat uygulanan sıçanlara taflan meyve ekstresinin ön- ve sonraki muamelesi veya vitamin C’nin ön muamelesi akciğer hasarında bir iyileşme gösterdi. Sonuç olarak, taflan meyve ekstresi veya vitamin C muamelesi, dimetoat toksisitesinin indüklediği akciğer toksisitesine karşı koruma sağlayabilir.

Anahtar Kelimeler

• Toksisite, dimetoat, akciğer, histopatoloji, taflan meyvesi.

Kaynakça

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