RATLARDA KARBON TETRAKLORUR İLE OLUŞTURULAN KRONİK KARACİĞER HASARI ÜZERİNE ZERDEÇAL(CURCUMA LONGA) VE MERYEMANA DİKENİNİN (SILYBUM MARIANUM) ETKİSİ

Öz

Çalışmada karbon tetraklorür (CCl4) ile ratlarda oluşturulan karaciğer hasarına karşı zerdeçal ve meryemana dikeninin lipid peroksidasyonu ve bazı biyokimyasal parametreler üzerine koruyucu etkinliği amaçlandı. Çalışmada Wistar albino 4 aylık, 60 adet ortalama 250 gr’lık rat kullanıldı. Kontrol grubuna tedavi uygulaması üç grup altında yapıldı. Birinci gruba haftada iki kere mısır yağı, V. gruba 100 mg/kg meryemana dikeni, VI. gruba 80 mg/kg zerdeçal 12 hafta her gün gavajla verildi. Deney grubuna haftada 2 kez 0,5 ml/kg CCl4 intraperitoneal enjekte edildi. Tedavi gruplarında, tedavi 12 hafta, iki grup altında yapıldı. CCl4+meryemana dikeni ve CCl4+zerdeçal grublarında sırasıyla CCl4 enjeksiyonuyla eş zamanlı 100 mg/kg meryemana dikeni ve 80 mg/kg zerdeçal gavaj ile verildi. Deney sonunda karaciğerlerin histopatolojisi ve serum biyokimyasal parametreleri incelendi. Kontrol gruplarında karaciğerler normal doku yapısında gözlenirken, CCl4 verilen Grup II’de hepatositlerde dejenerasyon ve yağlanma, remark kordonlarında bozulma, portal bölgede çoğunluğunu lenfosit mononüklear hücre infiltrasyonları, özellikle portal bölgede belirgin, parankime yayılmış bağ dokusu artışıyla ilgili pseudolobulasyon gözlendi. CCl4+meryemana dikeni ve CCl4+zerdeçal gruplarında Grup II’de kine benzer karaciğer lezyonlarına rastlanıldı. Kontrol, meryemana dikeni ve zerdeçal gruplarına göre, CCl4 uygulanan Grup II, III ve IV serum alanin aminotransferaz (ALT), Aspartat aminotransferaz (AST), düşük dansiteli lipoprotein (LDL), karaciğer malondialdehit (MDA) ve nitrik oksit (NO) değerlerinde artış; yüksek dansiteli lipoprotein (HDL) ve albümin değerlerinde düşüş görüldü. CCl4 verilen Grup II’ye göre CCl4+meryemana dikeni ve CCl4+zerdeçal verilen grupların serum ve karaciğer MDA ve NO düzeylerinde anlamlı bir değişim gözlenmedi. Kontrol, meryemana dikeni ve zerdeçal verilen grupların değerleri karşılaştırıldığında serum biyokimya değerlerinde anlamlı bir değişme gözlenmedi. Oluşan karaciğer hasarının geri döndürülmesi amacıyla çalışmamızda kullanılan, zerdeçal ve meryemana dikeni uygulaması aynı maddelerin değişik dozlarda verilerek yapılacak yeni araştırmalar ve etkilerinin belirlenmesine ihtiyaç olduğu sonucunu çıkarmıştır

Anahtar Kelimeler

• Karaciğer hasarı, karbon tetraklorür, meryemana dikeni, rat, zerdeçal.

The Effects of Curcuma Longa and Silybum Marianum on Carbon Tetrachloride Induced Chronic Hepatic Damage in Rats

Öz

This study aims to evaluate the protective efficacy of Curcuma longa and Silybum marianum on lipid peroxidation and some serum biochemical parameters in liver damage induced with carbon tetrachloride (CCl4) in rats. In the study, 60 4 month old Wistar albino rats weighing 250gr on average were used. Application of the treatment in the control groups were performed with three groups. The group I was administered corn oil twice for one week, group V was administered 100 mg/kg silymarin and group VI was administrated 80 mg/kg turmeric for 12 weeks by oral gavage. In the experimental group, 0,5 ml/kg CCl4 was injected intraperitoneally twice a week (Group II). The application of the treatment was performed in two groups for 12 weeks in the treatment groups. While the 100 mg/kg silymarin was given in the CCl4+silymarin group by gavage (Group III), the 80 mg/kg turmeric was given in the CCl4+turmeric group following of the injection of 0,5 ml/kg CCl4 (Group IV). At the end of the study, all liver tissues were examined with both histopathological and serum biochemical parameters. While the normal tissue structure was observed in the control group in the histopathological examination of the experimental and control groups, it was shown that there were fatty degeneration in the hepatocytes, deterioration in the structure of remark cords, hepatocyte degeneration, mononuclear cell infiltration areas composed of majority lymphocytes in the portal area and parenchyma, especially at portal area increasing connective tissue spread to parenchyma due to formation of lobulation. The liver lesions were found in the CCl4+silymarin and CCl4+turmeric groups as in Group II. While serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), low density lipoprotein (LDL) levels, liver malondialdehyde (MDA) and nitric oxide (NO) were increased in the CCl4-treated groups; the levels of high-density lipoprotein (HDL) and albumin values were decreased compared to the control group. When compared with the CCl4-treated group, it was observed that there was no statistically significant difference in the serum levels of CCl4+silymarin and CCl4+turmeric groups. It was observed that the serum biochemistry parameters were not significant change in the silymarin and turmeric groups compared with control group. In this study it has been concluded that there is a need for future studies in which different doses of silymarin and turmeric are used to reverse the liver damage and determine their efficacy.

Anahtar Kelimeler

• Carbon tetrachloride, curcuma longa, hepatotoxicity, rat, silybum marianum

Kaynakça

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