FARKLI COUSINIA TÜRLERİNDE PSÖDO TARAKSASTEROL (Ψ-TARAKSASTEROL) BİLEŞİĞİNİN HPLC-PDA İLE MİKTAR TAYİNİ

Yıl 2021, Cilt: 30 Sayı: 1, 17 - 20, 09.04.2021

Leyla Paşayeva , Hanifa Fatullayev Şehmus Kılıç Osman Üstün Osman Tugay

https://doi.org/10.34108/eujhs.632581

Cited By: 1

https://izlik.org/JA78FR83UW

Öz

Cousinia Cass. cinsi Asteraceae familyasına ait geniş yayılış alanına sahip cinslerden biridir. Bu cins üzerinde yok denecek kadar az farmakolojik etki ve fitokimyasal çalışmalar bulunmaktadır. Psödotaraksasterol (ψ-taraksasterol) antinosiseptif, anti-enflamatuvar aktiviteye sahip pentasikliktriterpen yapısında bileşiktir. Bu çalışmada C. stenocephala bitkisinden izole edilen ψ-taraksasterol bileşiğinin yedi farklı Cousinia türünde HPLC-PDA metodu ile miktar tayini yapılmıştır. Elde edilen sonuçlara gore enyüksek ψ-taraksasterol miktarı C. davisiana, C. stenocephala ve C. ramosissima ekstrelerinde bulunmuştur (sırasıyla, 0.423±0.004, 0.395±0.004 ve 0.374±0.000 g/100gekstre). ψ-taraksasterol bileşiği bu çalışmada HPLC-PDA metodu ile bu cinste ilk defa tayin edilmiş ve bu sonuçların cinsin farklı türlerinin çeşitli biyolojik aktivite araştırmalarında faydalı olacağı düşünülmektedir.

Anahtar Kelimeler

Asteraceae , Cousinia , HPLC-PDA , pentasiklik triterpene

Proje Numarası

THD-2019-8713

HPLC-PDA DETERMINATION OF PSEUDO TARAXASTEROL (Ψ-TARAXASTEROL)IN DIFFERENT COUSINIA SPECIES

https://izlik.org/JA78FR83UW

Öz

Cousinia Cass. is one of the widespread genera of Asteraceae family. There is a large number ofpharmacological activity studies on this genus and it has not yet been examined phytochemically in detail. Pseudo taraxasterol (ψ-taraxasterol) is a pentacyclic triterpene with different pharmacological activity such as antinociceptive, anti-inflammatory. In this report, we aimed to evaluate the ψ-taraxasterolcontent, which isolated from C. stenocephala in seven Cousiniaspecies by high-performance liquid chromatography-photodiode array detector (HPLC-PDA)method. According to quantitative analyses the highest content of ψ-taraxasterol was detected in C. davisiana, C. stenocephala, and C. ramosissima extracts (0.423±0.004, 0.395±0.004 and 0.374±0.000 g/100gextract, respectively). This is the first report on the quantification of pseudo taraxasterol by HPLC-PDA method in this genus, and this result will shed new lights on the advanced biological activity studies on species of this genus.

Anahtar Kelimeler

Asteraceae , Cousinia , HPLC-PDA , pentacyclic triterpene

Destekleyen Kurum

Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi

Proje Numarası

THD-2019-8713

Teşekkür

The authors are grateful to the Erciyes University Scientific Research Projects Coordinating Unit (BAP, project number THD-2019-8713) for financial support and Erciyes University Ziya Eren Drug Application and Research Center (ERFARMA) for providing HPLC facility.

Kaynakça

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