PARVOVİRUS İLE DOĞAL ENFEKTE KÖPEKLERDE MATRİKS METALLOPROTEİNAZ-9 EKSPRESYONU

Yıl 2018, Cilt: 27 Sayı: 1, 60 - 63, 01.03.2018

Mehmet Önder Karayiğit Özhan Karataş

Öz

Köpek parvoviral enteritis 1978 yılında ortaya çıktığından bu zamana kadar genç köpeklerde yaygın olarak
görülmektedir. Sindirim yolu ile alındıktan sonra sindirim kanalında ve kemik iliğinde mitotik aktivitesi yüksek hücreleri tercih ederek şiddetli kusma, ishal, ateş,
dehidrasyon ve halsizliğe sebep olur. MMP’ler
embriyonik gelişim, farklılaşma, proliferasyon ve doku
rejenerasyonunda önemli rol oynarlar. Bununla birlikte
MMP’leri aktive ve kontrol eden faktörler arasında bir
dengesizlik oluştuğunda bağırsak yangısı, artrit,
ateroskleroz veya kanser dahil bir çok hastalık ile sonuçlanır. Önemli olarak, MMP-9'un ekspresyon seviyesi
bağırsak yangı modellerinde ve Crohn hastalığı veya
ülseratif kolit gibi yangısal bağırsak hastalıkları olan
insanlarda daha yüksektir. Bu çalışmanın amacı, MMP9'un parvovirus ile enfekte köpeklerin bağırsak enfeksiyonları üzerindeki rolünü araştırmaktır. Bu çalışmada,
parvovirus ile doğal enfekte 21 köpek bağırsak dokusu
MMP-9 antikoru ile boyandı ve beş adet normal sağlıklı
köpek dokusu kontrol olarak kullanıldı. Sonuç olarak
parvovirus ile enfekte köpeklerin bağırsak dokularından MMP-9 ekspresyonu gerçekleşirken kontrol dokularda ekspresyon gözlenmedi. Bu sonuçlar köpeklerde
parvovirüs ile oluşan enteritisin patogenezinde MMP-9
ekspresyonunun etkisinin olabileceğini işaret etmektedir.

Anahtar Kelimeler

Parvoviral enteritis, MMP-9, köpek

Matrıx Metalloproteınase-9 Expressıon In Natural Infected Dogs Wıth Parvovırus

Öz

Canine parvoviral enteritis has, since its emergence in
1978, been common in young dogs. After ingestion, the
virus attacks rapidly dividing cells in the intestinal tract
and bone marrow, causing severe vomiting, diarrhoea,
fever, rapid dehydration, lethargy and decreased
activity. MMPs are crucial for embryonic development,
differentiation, proliferation, and regeneration of
tissues. An imbalance between MMPs activating and
inhibiting factors, however, results in a plethora of
diseases including intestinal inflammation, arthritis,
atherosclerosis or cancer. Importantly, expression
levels of MMP-9 is upregulated in intestinal
inflammation models and in patients suffering from
human inflammatory bowel diseases such as Crohn’s
disease or ulcerative colitis. The aim of this study is to
investigate the role of MMP-9 in the intestinal infection
of dogs with parvovirus. In this study, intestinal tissues
of 21 dog naturally infected with parvovirus were
stained with MMP-9 antibody and five normal healty
dog tissues were used as control. As a result, MMP-9
expression from intestinal tissues of dogs with
parvovirus-infected occurred while control tissues did
not expression. These results indicate that MMP-9
expression might have an impact on the pathogenesis of
parvovirus-induced enteritis in dogs.

Anahtar Kelimeler

Parvoviral enteritis, MMP-9, dog

Kaynakça

• 1. Decaro N, Desario C, Addie DD, et al. The study molecular epidemiology of canine parvovirus. Europe Emerg Infect Dis 2007;13:1222–1224.
• 2. Hoelzer K, Shackelton LA, Parrish CR, et al. Phylogenetic analysis reveals the emergence, evolution and dispersal of carnivore parvoviruses. J Gen Virol 2008;89:2280–2289.
• 3. Macintire DK, Smith-Carr S. Canine parvovirus. Part II. Clinical signs, diagnosis, and treatment. Comp Cont Educ Prac 1997; 19: 291-302.
• 4. Turk J, Miller M, Brown T, et al. Coliform septicemia and pulmonary disease associated with canine parvoviral enteritis: 88 cases (1987-1988). J Am Vet Med Assoc 1990;196: 771-773.
• 5. Meunier PC, Cooper BJ, Appel MJ, et al. Pathogenesis of canine parvovirus enteritis: The importance of viremia. Vet Pathol 1985;22:60–71.
• 6. Goetzl EJ, Banda MJ, Leppert D. Matrix metalloproteinases in immunity. J Immunol 1996;156:1–4.
• 7. B r i n c k e r h o f f C E , M a t r i s i a n L M . Matrixmetalloproteinases: a tail of a frog that became a prince. Nat Rev Mol Cell Biol 2002; 3:207– 214.
• 8. Birkedal-Hansen H, Moore WG, Bodden MK, et al. Matrixmetalloproteinases: a review. Crit Rev Oral Biol Med 1993;4:197–250.
• 9. Nelson AR, Fingleton B, Rothenberg ML, et al. Matrixmetalloproteinases:biologic activity and clinical implications. J Clin Oncol 2000; 18:1135– 1149.
• 10. William CS, Carole L, Wilson S, et al. Matrix metalloproteinases as modulators of inflammation and innate immunity. Immonology 2014; 4:617-629
• 11. Salmela MT, MacDonald TT, Black D, et al. Upregulation of matrix metalloproteinases in a model of T cell mediated tissue injury in the intestinal: analysis by genearray and in situ hybridisation. İntestinal 2002; 51:540–547.
• 12. Munoz M, Heimesaat MM, Danker K, et al. Interleukin (IL)-23 mediates Toxoplasma gondii induced immunopathology in the intestinal via matrixmetalloproteinase- 2 and IL-22 but independent of IL-17. J Exp Med 2009;206:3047– 3059.
• 13. Heimesaat MM, Dunay IR, Fuchs D, et al. The distinct roles of MMP-2 and MMP-9 in acute DSS colitis. Eur J Microbiol Immunol (Bp) 2011; 1:302–310.
• 14. von LB, Barthel B, Coupland SE, et al. Differential expression of matrix metalloproteinases and their tissue inhibitors in colon mucosa of patients with inflammatory bowel disease. İntestinal 2000; 47:63– 73.
• 15. Meijer MJ, Mieremet-Ooms MA, van der Zon AM, et al. Increased mucosal matrix metalloproteinase-1, - 2, -3 and −9 activity in patients with inflammatory bowel disease and the relation with Crohn's disease phenotype. Dig Liver Dis 2007; 39:733–739.
• 16. Pender SL, Li CK, Di Sabatino A, et al. Role of macrophage metalloelastase in intestinal inflammation. Ann N Y Acad Sci 2006; 1072:386– 388.
• 17. Santana A, Medina C, Paz-Cabrera MC, et al. Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice. World J Gastroenterol 2006; 12:6464–6472.
• 18. David MR, Andrew JS, Steve PH, et al. Matrix metalloproteinase 9 contributes to intestinal microbe homeostasis in a model of infectious colitis. BMC Microbiol 201212:105.
• 19. Bailey CJ, Hembry RM, Alexander A, et al. Distribution of the matrix metalloproteinases stromelysin, gelatinasesA and B, and collagenase in Crohn’s disease and normal intestine. J Clin Pathol 199447:113–116.
• 20. Baugh MD, Perry MJ, Hollander AP, et al. Matrix metalloproteinase levels are elevated in inflammatory bowel disease. Gastroenterology 1999; 117:814–822.
• 21. Louis E, Ribbens C, Godon A, et al. Increased production ofmatrixmetalloproteinase-3 and tissue inhibitor of metalloproteinase-1 by inflamed mucosa in inflammatory bowel disease. Clin Exp Immunol 2000; 120:241–246.
• 22. Hongchun L, Neal RP, Lewins W, et al. Constitutive expression of MMP9 in intestinal epithelium worsens murine acute colitis and is associated with increased levels of proinflammatory cytokine Kc. Am J Physiol Gastrointest Liver Physiol 2013; 304:793–803.
• 23. Marie EA, Ursula G, André F, et al. The role of gelatinases in campylobacter jejuni infection of gnotobiotic mice. Eur J Microbiol Immunol 2015; 4:256–267.
• 24. Crawford HC, Matrisian LM. Mechanisms controlling the transcription of matrixmetalloproteinase genes in normal and neoplastic cells. Enzyme Protein 1996; 49:20–37.
• 25. Saren P, Welgus HG, Kovanen PT. TNF-alpha and IL1beta selectively induce expression of 92-kDa gelatinase by human macrophages. J Immunol 1996;157:4159–4165.