Kadmiyumla stres oluşturulan Citrobacter freundii’nin vitamin içeriği ve stres belirteçlerine selenyumun etkisi

Yıl 2021, Cilt: 33 Sayı: 2, 697 - 707, 15.09.2021

Muhammad Salihu Ibrahim , Meltem Çakmak , Dursun Özer , Fikret Karataş , Sinan Saydam

https://doi.org/10.35234/fumbd.947044

Öz

Bu çalışmada, Citrobacter freundii farklı konsantrasyonlarda kadmiyum içeren LB besiyerinde üretildi. Kadmiyum içeren besi yerine değişik konsantrasyonlarda selenyum katılarak, üretilen hücre konsantrasyonu, ve bazı biyokimyasal parametreler spektrofotometre ve HPLC ile belirlendi. Bakteriler 0 (kontrol), 75, 100, 125 ppm kadmiyum (Cd) içeren besi ortamlarında üretildi. Kontrol ile 75, 100 ve 125 ppm Cd içeren gruplar karşılaştırıldığında, bakteri, vitaminler ve indirgenmiş glutatyonun (GSH) konsantrasyonlarında azalma, yükseltgenmiş glutatyon (GSSG), malondialdehit (MDA) ve 4-hidroksineoneal (4-HNE) konsantrasyonlarında ise artış gözlendi (p<0.05). 75, 100 ve 125 ppm Cd içeren besi ortamına 1.0 ve 3.0 ppm selenyum (Se) katılmasıyla birlikte, bakteri, vitaminler ve GSH konsantrasyonlarında artış, GSSG, MDA ve 4-HNE konsantrasyonlarında ise azalma gözlendi. Bu bulgulardan, kadmiyumun olumsuz etkisini, bakteri besi yerine katılan selenyumun azalttığı söylenebilir

Anahtar Kelimeler

Citrobacter freundii, kadmiyum, selenyum, vitaminler

Destekleyen Kurum

Fırat Üniversitesi

Proje Numarası

FUBAP FF.18.15

Teşekkür

Fırat Üniversitesi, Bilimsel Araştırma Projeleri birimi (FÜBAP) tarafından FF 18.15 numaralı proje ile maddi olarak desteklenmiştir

Kaynakça

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